Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Draft Genome Sequence of Mycobacterium abscessus Treated with a Fluoroquinolone in a Time-Dependent Manner

  • Du-Gyeong Han (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Ji-A Jeong (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Sung-Kyoung Lee (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Seong-Han Kim (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Se-Mi Jeon (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency)
  • Received : 2024.03.12
  • Accepted : 2024.05.13
  • Published : 2024.06.28
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Abstract

This study aimed to confirm the induction of resistance to other drug classes by treating Mycobacterium abscessus with moxifloxacin, a fluoroquinolone used for treating nontuberculous mycobacteria infection, and to obtain genetic data for improving treatment. The reads were assembled and analyzed using reference strain sequence data, and the whole-genome and transcriptome sequences of four strains (MD2, MD4, MD6, and MD8) were reported. Antibiotic resistance was not induced by moxifloxacin treatment; however, transcriptomic analysis revealed that the expression of genes responding to stress was upregulated.

Keywords

Acknowledgement

This research was supported by the "Korea National Institute of Health" research project No. 2021-NI-014-01 and 2022-NI-013-01.

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