Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves

  • Xueqin Xu (China Tobacco Guangxi Industrial Co., Ltd.) ;
  • Qianqian Wang (Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences) ;
  • Longyan Yang (China Tobacco Guangxi Industrial Co., Ltd.) ;
  • Zhiyan Chen (China Tobacco Guangxi Industrial Co., Ltd.) ;
  • Yun Zhou (China Tobacco Guangxi Industrial Co., Ltd.) ;
  • Hui Feng (Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences) ;
  • Peng Zhang (Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences) ;
  • Jie Wang (Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences)
  • Received : 2024.04.17
  • Accepted : 2024.06.04
  • Published : 2024.08.28
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Abstract

The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by Pichia pastoris, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in tobacco leaf decreased by 17.60%, 28.91% and 16.05%, respectively. The microbial community structure changed significantly, with Aspergillus abundance decreasing significantly, while Filobasidum, Cladosporium, Bullera, Komagataella, etc., increased in CBHA treated group. Soluble sugar was most affected by microbial community in tobacco leaves, which was negatively correlated with starch, cellulose and total nitrogen. During the fermentation process, the relative abundance of metabolism-related functional genes increased, and the expressions of cellulase and endopeptidase also increased. The results showed that the changes of bacterial community and dominant microbial community on tobacco leaves affected the content of chemical components in tobacco leaves, and adding CBHA for fermentation had a positive effect on improving the quality of tobacco leaves.

Keywords

Acknowledgement

This work was supported by grants from Science and Technology Project of Guangxi (2022450000340061), Science and Technology Cooperation Project of Shandong and Gansu (YDZX2022162) and Major science and technology project of China National Tobacco Corporation [110202201005 (JY-05)].

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