Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Small Plaque Variant Derived from Genotype V Japanese Encephalitis Virus Clinical Isolate K15P38

  • Woo-Jin Kim (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea) ;
  • Ah-Ra Lee (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea) ;
  • Su-Yeon Hong (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea) ;
  • Sang-Hyun Kim (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea) ;
  • Jae-Deog Kim (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea) ;
  • Sung Jae Kim (Vaxdigm Co., Ltd.) ;
  • Jae Sang Oh (Department of Neurosurgery, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Sang-Mu Shim (Division of Acute Viral Disease, Center for Emerging Virus Research, National Institute of Infectious Diseases, Korea National Institute of Health) ;
  • Sang-Uk Seo (Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea)
  • Received : 2024.04.30
  • Accepted : 2024.06.03
  • Published : 2024.08.28
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Abstract

Genotype V (GV) Japanese encephalitis virus (JEV) has been predominantly reported in the Republic of Korea (ROK) since 2010. GV JEV exhibits higher virulence and distinct antigenicity compared to other genotypes, which results in reduced efficacy of existing vaccines. Research on GV JEV is essential to minimize its clinical impact, but the only available clinical strain in the ROK is K15P38, isolated from the cerebrospinal fluid of a patient in 2015. We obtained this virus from National Culture Collection for Pathogens (NCCP) and isolated a variant forming small plaques during our research. We identified that this variant has one amino acid substitution each in the PrM and NS5 proteins compared to the reported K15P38. Additionally, we confirmed that this virus exhibits delayed propagation in vitro and an attenuated phenotype in mice. The isolation of this variant is a critical reference for researchers intending to study K15P38 obtained from NCCP, and the mutations in the small plaque-forming virus are expected to be useful for studying the pathology of GV JEV.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) (grant numbers: 2022R1F1A1074547) and the Korea National Institute of Health (KNIH) (project number: 2024ER170100).

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