Journal of Microbiology and Biotechnology

  • 제34권7호
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  • Pages.1464-1474
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Effects of Short-Term Tillage on Rhizosphere Soil Nitrogen Mineralization and Microbial Community Composition in Double-Cropping Rice Field

  • Haiming Tang (Hunan Soil and Fertilizer Institute) ;
  • Li Wen (Hunan Soil and Fertilizer Institute) ;
  • Kaikai Cheng (Hunan Soil and Fertilizer Institute) ;
  • Chao Li (Hunan Soil and Fertilizer Institute) ;
  • Lihong Shi (Hunan Soil and Fertilizer Institute) ;
  • Weiyan Li (Hunan Soil and Fertilizer Institute) ;
  • Yong Guo (Hunan Soil and Fertilizer Institute) ;
  • Xiaoping Xiao (Hunan Soil and Fertilizer Institute)
  • 투고 : 2024.02.01
  • 심사 : 2024.05.08
  • 발행 : 2024.07.28
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초록

Soil extracellular enzyme plays a vital role in changing soil nitrogen (N) mineralization of rice field. However, the effects of soil extracellular enzyme activities (EEA) and microbial community composition response to N mineralization of rice field under short-term tillage treatment needed to be further explored. In this study, we investigated the impact of short-term (8-year) tillage practices on rhizosphere soil N transformation rate, soil enzyme activities, soil microbial community structure, and the N mineralization function gene abundances in double-cropping rice field in southern China. The experiment consisted of four tillage treatments: rotary tillage with crop straw input (RT), conventional tillage with crop straw input (CT), no-tillage with crop straw retention (NT), and rotary tillage with all crop straw removed as a control (RTO). The results indicated that the rhizosphere soil N transformation rate in paddy field under the NT and RTO treatments was significantly decreased compared to RT and CT treatments. In comparison to the NT and RTO treatments, soil protease, urease, β-glucosaminidase, and arginase activities were significantly improved by the CT treatment, as were abundances of soil sub, npr, and chiA with CT and RT treatments. Moreover, the overall diversity of soil bacterial communities in NT and RTO treatments was significantly lower than that in RT and CT treatments. Soil chitinolytic and bacterial ureolytic communities were also obviously changed under a combination of tillage and crop straw input practices.

키워드

과제정보

This study was supported by Hunan Provincial Natural Science Foundation of China (2022JJ30352), National Natural Science Foundation of China (U21A20187), National Key Research and Development Project of China (2023YFD2301403), Hunan Science and Technology Talent Lift Project (2022TJ-N07), and Special Funds for the Construction of Innovative Provinces in Hunan Province (2023NK2027).

참고문헌

  1. Spiertz JHJ. 2010. Nitrogen, sustainable agriculture and food security. A review. Agron. Sustain. Dev. 30: 43-55. 
  2. Liu X, Peng C, Zhang W, Li S, An T, Xu Y, et al. 2022. Subsoiling tillage with straw incorporation improves soil microbial community characteristics in the whole cultivated layers: a one-year study. Soil Till. Res. 215: 105188. 
  3. Zhang H, Shi Y, Dong Y, Lapen DR, Liu J, Chen W. 2022. Subsoiling and conversion to conservation tillage enriched nitrogen cycling bacterial communities in sandy soils under long-term maize monoculture. Soil Till. Res. 215: 105197. 
  4. Tang HM, Li C, Cheng KK, Shi LH, Wen L, Li WY, et al. 2021. Effects of different tillage management on rhizosphere soil nitrogen mineralization and its extracellular enzyme activity in a double-cropping rice paddy field of southern China. Land Degrad. Dev. 32: 4933-4943. 
  5. Pittelkow CM, Linquist BA, Lundy ME, Liang X, van Groenigen, KJ Lee, et al. 2015. When does no-till yield more? A global meta-analysis. Field Crop Res. 183: 156-168. 
  6. Canisares LP, Grove J, Miguez F, Poffenbarger H. 2021. Long-term no-till increases soil nitrogen mineralization but does not affect optimal corn nitrogen fertilization practices relative to inversion tillage. Soil Till. Res. 213: 105080. 
  7. Mahal NK, Castellano MJ, Miguez FE. 2018. Conservation agriculture practices increase potentially mineralizable nitrogen: a meta-analysis. Soil Sci. Soc. Am. J. 82: 1270-1278. 
  8. Li XF, Hou LJ, Liu M, Lin XB, Li Y, Li SW. 2015. Primary effects of extracellular enzyme activity and microbial community on carbon and nitrogen mineralization in estuarine and tidal wetlands. Appl. Microbiol. Biotechnol. 99: 2895-2909. 
  9. Jalali M, Mahdavi S, Ranjbar F. 2014. Nitrogen, phosphorus and sulfur mineralization as affected by soil depth in rangeland ecosystems. Environ. Earth Sci. 72: 1775-1788. 
  10. Whalen JK, Kernecker ML, Thomas BW, Ngosong C, Sachdeva V. 2013. Soil food web controls on nitrogen mineralization are influenced by agricultural practices in humid temperate climates. CAB Rev. 8: 1-18. 
  11. Tabatabai MA, Ekenler M, Senwo ZN. 2010. Significance of enzyme activities in soil nitrogen mineralization. Commun. Soil Sci. Plant Anal. 41: 595-605. 
  12. Acosta-Martinez V, Tabatabai MA. 2000. Arylamidase activity of soils. Soil Sci. Soc. Am. J. 64: 215-221. 
  13. Iqbal A, Khan A, Green SJ, Ali I, He L, Zeeshan M, et al. 2021. Long-term straw mulching in a no-till field improves soil functionality and rice yield by increasing soil enzymatic activity and chemical properties in paddy soils. J. Plant Nutr. Soil Sci. 184: 622-634. 
  14. Nevins CJ, Lacey C, Armstrong S. 2021. Cover crop enzyme activities and resultant soil ammonium concentrations under different tillage systems. Eur. J. Agron. 126: 126277. 
  15. Carlos FS, Schaffer N, Marcolin E, Fernandes RS, Camargo FADO. 2021. Long-term no-tillage system can increase enzymatic activity and maintain bacterial richness in paddy field. Land Degrad. Dev. 32: 2257-2268. 
  16. Jahangir MMR, Nitu TT, Uddin S, Siddaka A, Sarker P, Khan S, et al. 2021. Carbon and nitrogen accumulation in soils under conservation agriculture practices decreases with nitrogen application rates. Appl. Soil Ecol. 168: 104178. 
  17. Khorsandi N, Nourbakhsh F. 208. Prediction of potentially mineralizable N from amidohydrolase activities in a manure-applied, corn residue-amended soil. Eur. J. Soil Biol. 44: 341-346. 
  18. Yang XY, Ren WD, Sun BH, Zhang SL. 2012. Effects of contrasting soil management regimes on total and labile soil organic carbon fractions in a loess soil in China. Geoderma 177-178: 49-56. 
  19. Tang HM, Xiao XP, Li C, Tang WG, Cheng KK, Pan XC, et al. 2019. Effects of different soil tillage systems on soil carbon management index under double-cropping rice field in southern China. Agron. J. 111: 440-446. 
  20. Tang HM, Xiao XP, Li C, Tang WG, Pan XC, Cheng KK, et al. 2020. Impact of tillage practices on soil aggregation and humic substances under double-cropping paddy field. Agron. J. 112: 624-632. 
  21. Stark JM, Hart SC. 1996. Diffusion technique for preparing salt solutions, Kjeldahl digests, and persulfate digests for nitrogen-15 analysis. Soil Sci. Soc. Am. J. 60: 1846-1855. 
  22. Zhang Q, Liang G, Zhou W, Sun J, Wang X, He P. 2016. Fatty-acid profiles and enzyme activities in soil particle-size fractions under long-term fertilization. Soil Sci. Soc. Am. J. 80: 97-111. 
  23. Ouyang Y, Norton JM. 2020. Short-term nitrogen fertilization affects microbial community composition and nitrogen mineralization functions in an agricultural soil. Appl. Environ. Microbiol. 86: e02278-19. 
  24. Fish JA, Chai B, Wang Q, Sun Y, Brown CT, Tiedje JM, et al. 2013. FunGene: the functional gene pipeline and repository. Front. Microbiol. 4: 291. 
  25. Herbold CW, Pelikan C, Kuzyk O, Hausmann B, Angel R, Berry D, et al. 2015. A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes. Front. Microbiol. 6: 731. 
  26. Price MN, Dehal PS, Arkin AP. 2010. FastTree 2-approximately maximum-likelihood trees for large alignments. PLoS One 5: e9490. 
  27. McMurdie PJ, Holmes S. 2013. Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS One 8: e61217. 
  28. Edgar RC. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat. Methods 10: 996-998. 
  29. SAS. SAS Software of the SAS System for Windows. SAS Institute Inc, Cary, NC, USA. 2008. 
  30. Mohanty M, Reddy SK, Probert ME, Dalal RC, Rao SA, Menzies NW. 2011. Modelling N mineralization from green manure and farmyard manure from a laboratory incubation study. Ecol. Model. 222: 719-726. 
  31. Nannipieri P, Giagnoni L, Renella G, Puglisi E, Ceccanti B, Masciandaro G, 2012. Soil enzymology: classical and molecular approaches. Biol. Fertil Soils 48: 743-762. 
  32. Bach HJ, Hartmann A, Schloter M, Munch JC. 2001. PCR primers and functional probes for amplification and detection of bacterial genes for extracellular peptidases in single strains and in soil. J. Microbiol. Methods 44: 173-182. 
  33. Collier JL, Baker KM, Bell SL. 2009. Diversity of urea-degrading microorganisms in open-ocean and estuarine planktonic communities. Environ. Microbiol. 11: 3118-3131. 
  34. Tang HM, Li C, Cheng KK, Shi LH, Wen L, Li WY, et al.2022. Effects of short-term soil tillage practice on activity and community structure of ammonia-oxidizing bacteria and archaea under the double-cropping rice field. J. Appl. Microbiol. 132: 1307-1318. 
  35. Daims H, Lebedeva EV, Pjevac P, Han P, Herbold C, Albertsen M, et al. 2015. Complete nitrification by Nitrospira bacteria. Nature 528: 504-509. 
  36. Chang F, Jia F, Lv R, Li Y, Wang Y, Jia Q, et al. 2021. Soil bacterial communities reflect changes in soil properties during the tillage years of newly created farmland on the loess plateau. Appl. Soil Ecol. 61: 103853.