Journal of Microbiology and Biotechnology

  • 제34권7호
  • /
  • Pages.1410-1418
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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The In Vitro Promoting Angiogenesis Roles of Exosomes Derived from the Protoscoleces of Echinococcus multilocularis

  • Wenjing Zhou (Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University) ;
  • Xiang Li (Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University) ;
  • Xinqi Yang (Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University) ;
  • Bin Ye (Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University)
  • 투고 : 2024.03.21
  • 심사 : 2024.05.13
  • 발행 : 2024.07.28
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초록

Alveolar echinococcosis (AE) is a persistent parasite condition that causes the formation of tumorlike growths. It is a challenge to treat the disease. These growths need neovascularization to get their oxygen and nutrients, and the disease is prolonged and severe. Considerable research has been conducted on exosomes and their interactions with Echinococcus multilocularis in the context of immunological evasion by the host. However, the extent of their involvement in angiogenesis needs to be conducted. The primary objective of this investigation was to preliminarily explore the effect of exosomes produced from E. multilocularis protoscoleces (PSC-exo) on angiogenesis, to elucidate the mechanism of their roles in the regulation of the downstream pathway of VEGFA activation, and to provide ideas for the development of novel treatments for AE. The study evaluated the impact of PSC-exo increases proliferation, migration, invasion, and tube formation of HUVECs at concentrations of up to 50 ㎍/ml. In addition, the study sought to validate the findings in vivo. This effect involved increased VEGFA expression at gene and protein levels and AKT/mTOR pathway activation. PSC-exo are crucial in promoting angiogenesis through VEGFA upregulation and AKT/mTOR signaling. This research contributes to our knowledge of neovascularization in AE.

키워드

과제정보

The authors would like to thank the Research Center for Molecular Medicine and Tumor at Chongqing Medical University for providing laboratory equipment. This work was partially supported by the National Natural Science Foundation of China (grant no. 81672045).

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