Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지
DOI QR코드

DOI QR Code

Potential of Erythrosine-Mediated Photodynamic Therapy as a Cavity Disinfectant: Antibacterial Efficacy and Bonding Ability

  • Gawon Lee (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Haeni Kim (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Siyoung Lee (Department of Microbiology and Immunology, College of Dentistry, Gangneung-Wonju National University) ;
  • Juhyun Lee (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2024.06.25
  • Accepted : 2024.08.12
  • Published : 2024.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to evaluate the antibacterial efficacy of erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans (S. mutans) in dentin and its effect on the shear bond strength (SBS) of composite resin to dentin. Eighty extracted human noncarious premolars were used in this study. Forty teeth were used for the antibacterial activity test, while the remaining 40 were used for the SBS test. Both experiments were conducted with 4 experimental groups (n = 10): control (distilled water), sodium hypochlorite (NaOCl, 6%), chlorhexidine (CHX, 0.12%), and erythrosine-mediated PDT. Antibacterial effects were evaluated by counting S. mutans colony-forming units (CFUs). The SBS of composite resins to dentin was measured using a universal testing machine. All treatments (NaOCl, CHX, and PDT) demonstrated statistically significant differences in antibacterial activity compared with the control group (p < 0.05). The antibacterial effects were ranked from strongest to weakest as follows: NaOCl, PDT, and CHX. In the SBS test, the NaOCl group exhibited a statistically significant difference compared with the CHX, PDT, and control groups (p < 0.05), with the lowest bond strength. No statistically significant differences were found among the CHX, PDT, and control groups (p > 0.05). Erythrosine-mediated PDT exhibited significant antibacterial effects against S. mutans, with higher antibacterial activity than CHX but lower than NaOCl. Only NaOCl negatively affected the bond strength of composite resin to dentin. In conclusion, erythrosine-mediated PDT shows potential as a cavity disinfectant due to its significant antibacterial effects against S. mutans and lack of adverse effects on bond strength.

Keywords

Acknowledgement

This paper was supported by research funds for newly appointed professors of Gangneung-Wonju National University in 2023.

References

  1. GBD 2016 Disease and Injury Incidence and Prevalence Collaborators : Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet, 390:1211-1259, 2017. https://doi.org/10.1016/S0140-6736(17)32154-2
  2. Boston DW, Graver HT : Histological study of an acid red caries-disclosing dye. Oper Dent, 14:186-192, 1989.
  3. Brannstrom M : The cause of postoperative sensitivity and its prevention. J Endod, 12:475-481, 1986. https://doi.org/10.1016/S0099-2399(86)80202-3
  4. Imazato S, Torii Y, Takatsuka T, Inoue K, Ebi N, Ebisu S : Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. J Oral Rehabil, 28:314-319, 2001. https://doi.org/10.1046/j.1365-2842.2001.00659.x
  5. Hashem AA, Ghoneim AG, Lutfy RA, Fouda MY : The effect of different irrigating solutions on bond strength of two root canal-filling systems. J Endod, 35:537-540, 2009. https://doi.org/10.1016/j.joen.2009.01.003
  6. Haapasalo M, Shen Y, Qian W, Gao Y : Irrigation in endodontics. Dent Clin North Am, 54:291-312, 2010. https://doi.org/10.1016/j.cden.2009.12.001
  7. Costa ACBP, De Campos Rasteiro VM, Pereira CA, Da Silva Hashimoto ESH, Beltrame M Jr, Junqueira JC, Jorge AOC : Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy. Arch Oral Biol, 56:1299-1305, 2011. https://doi.org/10.1016/j.archoralbio.2011.05.013
  8. Campanholi KSS, Braga G, da Silva JB, da Rocha NL, de Francisco LMB, de Oliveira EL, Bruschi ML, de Castro-Hoshino LV, Sato F, Hioka N, Caetano W : Biomedical platform development of a chlorophyll-based extract for topic photodynamic therapy: mechanical and spectroscopic properties. Langmuir, 34:8230-8244, 2018. https://doi.org/10.1021/acs.langmuir.8b00658
  9. Lee YH, Park HW, Lee JH, Seo HW, Lee SY : The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci, 4:196-201, 2012. https://doi.org/10.1038/ijos.2012.63
  10. Pereira CA, Costa ACBP, Carreira CM, Junqueira JC, Jorge AOC : Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Lasers Med Sci, 28:859-864, 2013. https://doi.org/10.1007/s10103-012-1175-3
  11. Wood S, Metcalf D, Devine D, Robinson C : Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother, 57:680-684, 2006.
  12. Alkhudhairy F, Vohra F, Naseem M, Ahmad ZH : Adhesive bond integrity of dentin conditioned by photobiomodulation and bonded to bioactive restorative material. Photodiagnosis Photodyn Ther, 28:110-113, 2019. https://doi.org/10.1016/j.pdpdt.2019.08.014
  13. Ferracane JL : Resin composite - state of the art. Dent Mater, 27:29-38, 2011.
  14. Turkun M, Turkun LS, Kalender A : Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins. Quintessence Int, 35:469-476, 2004.
  15. Meiers JC, Kresin JC : Cavity disinfectants and dentin bonding. Oper Dent, 21:153-159, 1996.
  16. Perdigao J, Denehy GE, Swift EJ Jr : Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent, 7:81-84, 1994.
  17. Cao DS, Hollis RA, Christensen RP, Christensen GJ : Effect of tooth disinfecting procedures on dentin shear bond strength. J Dent Res, 74:73, 1995.
  18. Vianna ME, Gomes BP, Berber VB, Zaia AA, Ferraz CCR, de Souza-Filho FJ : In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 97:79-84, 2004. https://doi.org/10.1016/S1079-2104(03)00360-3
  19. McDonnell G, Russell AD : Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev, 12:147-179, 1999. https://doi.org/10.1128/CMR.12.1.147
  20. Arias-Moliz MT, Ferrer-Luque CM, Espigares-Garcy'a M, Baca P : Enterococcus faecalis biofilms eradication by root canal irrigants. J Endod, 35:711-714, 2009. https://doi.org/10.1016/j.joen.2009.01.018
  21. Chiniforush N, Pourhajibagher M, Shahabi S, Bahador A : Clinical approach of high technology techniques for control and elimination of endodontic microbiota. J Lasers Med Sci, 6:139-150, 2015. https://doi.org/10.15171/jlms.2015.09
  22. Castano AP, Demidova TN, Hamblin MR : Mechanisms in photodynamic therapy: Partone-Photosensitizers, photochemistry and cellular localization. Photodiagnosis Photodyn Ther, 1:279-293, 2004. https://doi.org/10.1016/S1572-1000(05)00007-4
  23. Vohra F, Akram Z, Safii SH, Vaithilingam RD, Ghanem A, Sergis K, Javed F : Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis: a systematic review. Photodiagnosis Photodyn Ther, 13:139-147, 2016. https://doi.org/10.1016/j.pdpdt.2015.06.010
  24. Hamblin MR, Abrahamse H : Oxygen-Independent Antimicrobial Photoinactivation: Type III Photochemical Mechanism? Antibiotics, 9:53, 2020.
  25. Araujo NC, Fontana CR, Bagnato VS, Gerbi MEM : Photodynamic effects of curcumin against cariogenic pathogens. Photomed Laser Surg, 30:393-399, 2012. https://doi.org/10.1089/pho.2011.3195
  26. Juric IB, Plecko V, Panduric DG, Anic I : The antimicrobial effectiveness of photodynamic therapy used as an addition to the conventional endodontic re-treatment: a clinical study. Photodiagnosis Photodyn Ther, 11:549-555, 2014. https://doi.org/10.1016/j.pdpdt.2014.10.004
  27. Metcalf D, Robinson C, Devine D, Wood S : Enhancement of erythrosine-mediated photodynamic therapy of Streptococcus mutans biofilms by light fractionation. J Antimicrob Chemother, 58:190-192, 2006. https://doi.org/10.1093/jac/dkl205
  28. Choi S, Park H, Lee J, Seo H, Lee S : Optimum treatment parameters for photodynamic antimicrobial chemotherapy on Streptococcus mutans biofilms. J Korean Acad Pediatr Dent, 42:151-157, 2015. https://doi.org/10.5933/JKAPD.2015.42.2.151
  29. Baab DA, Broadwell AH, Williams BL : A comparison of antimicrobial activity of four disclosant dyes. J Dent Res, 62:837-841, 1983. https://doi.org/10.1177/00220345830620071601
  30. Marshall GW Jr, Yucel N, Balooch M, Kinney JH, Habelitz S, Marshall SJ : Sodium hypochlorite alterations of dentin and dentin collagen. Surf Sci, 491:444-455, 2001. https://doi.org/10.1016/S0039-6028(01)01310-3
  31. Toledano M, Osorio R, Perdigao J, Rosales JI, Thompson JY, Cabrerizo-Vilchez MA : Effect of acid etching and collagen removal on dentin wettability and roughness. J Biomed Mater Res, 47:198-203, 1999. https://doi.org/10.1002/(SICI)1097-4636(199911)47:2<198::AID-JBM9>3.0.CO;2-L
  32. Hugo WB, Longworth AR : The effect of chlorhexidine on the electrophoretic mobility, cytoplasmic constituents, dehydrogenase activity and cell walls of Escherichia coli and Staphylococcus aureus. J Pharm Pharmacol, 18:569-578, 1966. https://doi.org/10.1111/j.2042-7158.1966.tb07935.x
  33. Perdigao J, Thompson JY, Toledano M, Osorio R : An ultra-morphological characterization of collagen-depleted etched dentin. Am J Dent, 12:250-255, 1999.
  34. Wakabayashi Y, Kondou Y, Suzuki K, Yatani H, Yamashita A : Effect of dissolution of collagen in adhesion to dentin. Int J Prosthodont, 7:302-306, 1994.
  35. Arias VG, Bedran-de-Castro AKB, Pimenta LA : Effects of sodium hypochlorite gel and sodium hypochlorite solution on dentin bond strength. J Biomed Mater Res B Appl Biomater, 72:339-344, 2005.
  36. Uno S, Finger WJ : Function of the hybrid zone as a stress-absorbing layer in resin-dentin bonding. Quintessence Int, 26:733-738, 1995.
  37. Ozturk B, Ozer F : Effect of NaOCl on bond strengths of bonding agents to pulp chamber lateral walls. J Endod, 30:362-365, 2004. https://doi.org/10.1097/00004770-200405000-00013
  38. Nikaido T, Takano Y, Sasafuchi Y, Burrow MF, Tagami J : Bond strengths to endodontically-treated teeth. Am J Dent, 12:177-180, 1999.
  39. Hawkins CL, Davies MJ : Hygochlorite-induced oxidation of proteins in plasma: Formation of chloramines and nitrogen-centred radicals and their role in protein fragmentation. Biochem J, 340:539-548, 1999. https://doi.org/10.1042/bj3400539
  40. Campos EA, Correr GM, Leonardi DP, Pizzatto E, Morais EC : Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Braz Oral Res, 23:340-345, 2009. https://doi.org/10.1590/S1806-83242009000300019
  41. Di Hipolito V, Rodrigues FP, Piveta FB, Azevedo LDA, Bruschi Alonso RC, Silikas N, Carvalho RM, De Goes MF, Perlatti D'Alpino PH : Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater, 28:495-501, 2012. https://doi.org/10.1016/j.dental.2011.11.027
  42. Castro FLA, Andrade MF, Duarte Junior SLL, Vaz LG, Ahid FJM : Effect of 2% chlorhexidine on microtensile bond strength of composite to dentin. J Adhes Dent, 5:129-138, 2003.
  43. Soares CJ, Pereira CA, Pereira JC, Santana FR, Prado CJ : Effect of chlorhexidine application on microtensile bond strength to dentin. Oper Dent, 33:183-188, 2008. https://doi.org/10.2341/07-69
  44. Chang YE, Shin DH : Effect of chlorhexidine application methods on microtensile bond strength to dentin in Class I cavities. Oper Dent, 35:618-623, 2010. https://doi.org/10.2341/09-360-L
  45. Hassan AM, Goda AA, Baroudi K : The effect of different disinfecting agents on bond strength of resin composites. Int J Dent, 2014:231235, 2014.
  46. Hebling J, Pashley DH, Tjaderhane L, Tay FR : Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res, 84:741-746, 2005. https://doi.org/10.1177/154405910508400811
  47. Demarco FF, Matos AB, Matson E, Powers JM : Dyes for caries detection influence sound dentin bond strength. Oper Dent, 23:294-298, 1998.