농업과학연구 (Korean Journal of Agricultural Science)

  • 제51권2호
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  • Pages.169-178
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  • 2024
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Effect of cordycepin-enriched Cordyceps militaris L. extract powder on alcohol degradation and hepatoprotection in mice alcohol model

  • Ju-Hye Kim (Department of Bio-Environmental Chemistry, College of Agriculture and Life Science, Chungnam National University) ;
  • Heejin Park (Department of Advanced Toxicology Research, Korea Institute of Toxicology (KIT)) ;
  • Mun-Hyoung Bae (Functional Materials Research Team, Rpbio Research Institute, Rpbio Co., Ltd.) ;
  • Youngha Seo (Functional Materials Research Team, Rpbio Research Institute, Rpbio Co., Ltd.) ;
  • Eun-Young Gu (Department of Advanced Toxicology Research, Korea Institute of Toxicology (KIT)) ;
  • Taek-Keun Oh (Department of Bio-Environmental Chemistry, College of Agriculture and Life Science, Chungnam National University) ;
  • Byoung-Seok Lee (Department of Advanced Toxicology Research, Korea Institute of Toxicology (KIT))
  • 투고 : 2024.03.20
  • 심사 : 2024.05.02
  • 발행 : 2024.06.01
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초록

Cordyceps militaris L. (C. militaris) has been traditionally used as tonic medicine for metabolic syndrome. Cordycepin, has been reported with immunomodulatory, antitumor, and hepatoprotective effect, is the main extract from C. militaris. This study was conducted to evaluate the alcohol degradation and hepatoprotective effect of cordycepin-enriched C. militaris extract (CM) powder in chronic and binge ethanol (ethanol Lieber-DeCarli diet)-fed male C57BL/6 Mice. Cordycepin-enriched C. militaris extract powder was orally administered once daily at dose levels of 0, 125, 250, and 500 mg·kg-1·day-1 for 16 days. For evaluating alcohol degradation, ethanol concentration and alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity were measured in serum. Serum ethanol (EtOH) concentration was decreased at CM treated groups, and the activities of ADH and ALDH were increased dose-dependently at CM treated groups compare to EtOH model group. In clinical chemistry, the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were lower in CM treated groups than those in EtOH model group. Additionally, absolute and relative (to body weight) liver weights were statistically decreased in the CM treated groups compared to the EtOH model group. In conclusion, our study showed that cordycepin-enriched C. militaris extract powder exhibits hepatoprotective effect by upregulating the ADH and ALDH enzyme in an alcoholic liver disease model.

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과제정보

본 연구는 안전성평가연구소(1711195885; B-S Lee)의 지원으로 수행되었습니다.

참고문헌

  1. Bertola A. 2020. Mouse model of alcoholic steatohepatitis. Kupffer Cells: Methods in Molecular Biology 2164:145-157. https://doi.org/10.1007/978-1-0716-0704-6_15
  2. Bertola A, Mathews S, Ki SH, Wang H, Gao B. 2013. Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nature Protocol 8:627-637. https://doi.org/10.1038/nprot.2013.032
  3. Bradford BU, Kono H, Isayama F, Kosyk O, Wheeler MD, Akiyama TE, Rusyn I. 2005. Cytochrome P450 CYP2E1, but not nicotinamide adenine dinucleotide phosphate oxidase, is required for ethanol-induced oxidative DNA damage in rodent liver. Hepatology 41:336-344. https://doi.org/10.1002/hep.20532
  4. Cha JY, Ahn HY, Cho YS, Je JY. 2013. Protective effect of cordycepin-enriched Cordyceps militaris on alcoholic hepatotoxicity in Sprague-Dawley rats. Food and Chemical Toxicology 60:52-57. https://doi.org/10.1016/j.fct.2013.07.033
  5. Eriksson CJP, Fukunaga T, Sarkola T, Chen WJ, Chen CC, Ju JM, Cheng AT, Yamamoto H, Kohlenberg-Muller K, Kimura M, et al. 2001. Functional relevance of human adh polymorphism. Alcohol, Clinical and Experimental Research 25:157-163. https://doi.org/10.1111/j.1530-0277.2001.tb02391.x
  6. Gillessen A, Schmidt HH. 2020. Silymarin as supportive treatment in liver diseases: A narrative review. Advances in Therapy 37:1279-1301. https://doi.org/10.1007/s12325-020-01251-y
  7. Jeong JW, Choi YH. 2015. Anti-cancer properties and relevant mechanisms of Cordycepin, an active ingredient of the insect fungus Cordyceps spp. Journal of Life Science 25:607-614. [in Korean] https://doi.org/10.5352/JLS.2015.25.5.607
  8. Jo WS, Nam BH, Oh SJ, Choi YJ, Kang EY, Hong SH, Lee SH, Jeong MH. 2008. Hepatic protective effect and single-dose toxicity study of water extract of Cordyceps militaris grown upon Protaetia dreujtarsis. Korean Journal of Food Science and Technology 40:106-110. [in Korean]
  9. Jung HD, Kim JD. 2021. Anti-oxidant and anti-inflammatory effects of Cordyceps militaris extracts. Journal of the Korean Society of Cosmetology 27:932-939. [in Korean] https://doi.org/10.52660/JKSC.2021.27.4.932
  10. Kim MS, Lee DH, Kang HS, Park HS, Jung S, Lee JW, Kwon KS, Kim PS, Kim HG, Shin YW, et al. 2004. Genetic polymorphisms of alcohol-metabolizing enzymes and cytokines in patients with alcohol induced pancreatitis and alcoholic liver cirrhosis. Korean Journal of Gastroenterol 43:355-363. [in Korean]
  11. Kim YJ. 2008. Interpretation of liver function tests. Korean Journal of Gastroenterol 51:219-224. [in Korean]
  12. Kim YS, Park JY, Kwon YB, Lim DW, Song MK, Choi HY, Kim HC. 2013. Hepatoprotective Effects of Hovenia dulcis extract on acute and chronic liver injuries induced by alcohol and carbon tetrachloride. Korean Journal of Herbology 28:25-32. [in Korean]
  13. Lieber CS. 1994. Alcohol and the liver. Gastroenterology 106:1105.
  14. Muriel P, Garciapina T, Perez-Alvarez V, Mourelle M. 1992. Silymarin protects against paracetamol-induced lipid peroxidation and liver damage. Journal of Applied Toxicology 12:439-442. https://doi.org/10.1002/jat.2550120613
  15. Sin HJ, Choung SY, Kang S, Kwon HT, Kim BH. 2023. Anti-alcohol and anti-aldehyde hangover effect of aldehyde dehydrogenase related compounds in rat. Journal of Environmental Health Sciences 49:99-107. [in Korean] https://doi.org/10.5668/JEHS.2023.49.2.99
  16. Yun MK, Jeong HC, Lee SJ, Lee S. 2023. Effect of lactobacillus fermented garlic extract powder on alcohol and acetaldehyde metabolism. Journal of the Korean Society of Food Science and Nutrition 52:357-362. [in Korean] https://doi.org/10.3746/jkfn.2023.52.4.357
  17. Zakhari S. 2006. Overview: How is alcohol metabolized by the body? Alcohol Res Health 29:245-254. https://doi.org/10.1159/000095013