Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
권호 표지
DOI QR코드

DOI QR Code

A report on 36 unrecorded bacterial species isolated from Korean islands in 2023

  • Seung Yeol Shin (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Yihyun Jeon (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Heeyoung Kang (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Myung Kyum Kim (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Dong-Uk Kim (Department of Biological Science, College of Science and Engineering, Sangji University) ;
  • Yochan Joung (Department of Biotechnology and Chemical Engineering, Gyeonggi University of Science and Technology) ;
  • Jaeho Song (Division of Microbiology, Honam National Institute of Biological Resources)
  • Received : 2024.02.28
  • Accepted : 2024.07.23
  • Published : 2024.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Various samples were collected from Korean islands in order to obtain unrecorded bacterial species in 2023. After aerobically incubating on marine agar and Reasoner's 2A agar, approximately 1,200 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 36 strains showed ≥98.7% sequence similarity to previously published and validated bacterial species. However, these strains have not previously been reported in the Republic of Korea, indicating that they belong to Korean unrecorded bacterial species. The unrecorded bacterial species were assigned to the classes Actinomycetes, Bacilli, Bacteroidia, Flavobacteriia, Sphingobacteriia, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. The information we obtained by examining the strains includes details of the Gram reactions, colony and cell morphology, biochemical characteristics, and phylogenetic positions of the unrecorded species.

Keywords

Acknowledgement

This study was supported by the "Survey of Indigenous Species in Korean Islands" research grant (HNIBR202101111; HNIBR202301108) awarded by the Honam National Institute of Biological Resources at the Ministry of Environment in Korea.

References

  1. Cho, H., Y. Lim, S. Kim, H. Jo, M. Kim and J.-C. Cho. 2023. A report of 20 unrecorded bacterial species isolated from the coastal area of Korean islands in 2022. Journal of Species Research 12(2):165-173. https://doi.org/10.12651/JSR.2023.12.2.165
  2. Chun, J., A. Oren, A. Ventosa, H. Christensen, D.R. Arahal, M.S. da Costa, A.P. Rooney, H. Yi, X.-W. Xu, S. De Meyer and M.E. Trujillo. 2018. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. International Journal of Systematic and Evolutionary Microbiology 68(1):461-466. https://doi.org/10.1099/ijsem.0.002516
  3. Felsenstein, J. 1981. Evolutionary Trees from Gene Frequencies and Quantitative Characters: Finding Maximum Likelihood Estimates. Evolution 35:1229-1242. https://doi.org/10.2307/2408134
  4. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  5. Fernandez-Palacios, J.M., H. Kreft, S.D.H. Irl, S. Norder, C. Ah-Peng, P.A.V. Borges, K.C. Burns, L. de Nascimento, J.Y. Meyer, E. Montes and D.R. Drake. 2021. Scientists' warning-The outstanding biodiversity of islands is in peril. Global Ecology and Conservation 31:e01847.
  6. Li, S., P. Wang, Y. Chen, M.C. Wilson, X. Yang, C. Ma, J. Lu, X. Chen, J. Wu, W. Shu and L. Jiang. 2020. Island biogeography of soil bacteria and fungi: similar patterns, but different mechanisms. The ISME Journal 14(7):1886-1896. https://doi.org/10.1038/s41396-020-0657-8
  7. Lim, M.S., B.H. Lee and S.J. Lee. 2012. Endemic Species of Korea. National Institute of Biological Resources, Incheon.
  8. National Institute of Biological Resources. 2020. Discovered 150 unrecorded plants in Jeju and islands in the Southwest Sea for 30 years. [Available from: https://www.nibr.go.kr/cmn/board/SYSTEM_DEFAULT000004/62571bbsDetail.do].
  9. Rzhetsky, A. and M. Nei. 1993. Theoretical foundation of the minimum-evolution method of phylogenetic inference. Molecular Biology and Evolution 10:1073-1095.
  10. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  11. Shin, S.Y., Y. Joung, D. Han, J.H. Jeong, Y.H. Jeon and J. Song. 2022. A report of 30 unrecorded bacterial species in Korea, isolated from marine ecosystems in 2021. Journal of Species Research 11(3):143-154. https://doi.org/10.12651/JSR.2022.11.3.143
  12. Shin, S., H. Jeon, S. Kim, H.J. Noh, J.W. Jo, K. Min and H. Son. 2023a. Two Previously Unrecorded Fungal Species Isolated from Muui Island in Korea. Mycobiology 51(6):410-416. https://doi.org/10.1080/12298093.2023.2290766
  13. Shin, S.Y., M.K. Kim, Y. Joung, Y.H. Jeon, J.H. Jeong, H.-J. Noh, J. Song and H. Kang. 2023b. A report on 10 unrecorded bacterial species isolated from the Korean islands in 2022. Journal of Species Research 11 (spc2):143-154.
  14. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38(7):3022-3027. https://doi.org/10.1093/molbev/msab120
  15. Yang, S.-J. and J.-C. Cho. 2008. Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. International Journal of Systematic and Evolutionary Microbiology 58(2):315318.
  16. Yoon, S.H., S.M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. International Journal of Systematic and Evolutionary Microbiology 67(5):1613-1617. https://doi.org/10.1099/ijsem.0.001755