Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Effects of extracellular vesicles (EVs) from uterine fluid during estrus and diestrus on porcine embryonic development

  • Shuntaro Miura (Department of Materials Science and Bioengineering, Nagaoka University of Technology) ;
  • Heejae Kang (College of Veterinary Medicine, Chungnam National University) ;
  • Seonggyu Bang (College of Veterinary Medicine, Chungnam National University) ;
  • Ayeong Han (College of Veterinary Medicine, Chungnam National University) ;
  • Islam M. Saadeldin (College of Veterinary Medicine, Chungnam National University) ;
  • Sanghoon Lee (College of Veterinary Medicine, Chungnam National University) ;
  • Koichi Takimoto (Department of Materials Science and Bioengineering, Nagaoka University of Technology) ;
  • Jongki Cho (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2024.06.02
  • Accepted : 2024.06.14
  • Published : 2024.06.30
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Abstract

Background: Porcine embryonic development is widely utilized in the medical industry. However, the blastocyst development rate in vitro is lower compared to in vivo. To address this issue, various supplements are employed. Extracellular vesicles (EVs) play the role of communicators that carry many bioactive cargoes. Additionally, the contents of EVs can vary on the estrous cycle. Methods: We compared the effects of adding EVs derived from porcine uterine fluid (UF), categorized as non-EV (G1), EVs in estrus (G2) and EVs in diestrus (G3). After in vitro culture (IVC) was performed in three different groups, cleavage rate and blastocyst development rate were examined. In addition, glutathione (GSH) and reactive oxygen species (ROS) levels were measured 2 days after activation to assess oxidative stress. Results: Using NTA and cryo-TEM, we confirmed the presence of EVs with sizes ranging from 30 nm to 200 nm, that the particles were suitable for analysis for analysis. In IVC data, the highest cleavage rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Similarly, the highest blastocyst development rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Conclusions: These results indicate that estrus derived EVs contain biofactors beneficial for early blastocyst development, including GSH which protects the blastocyst from oxidative stress. Additionally, although diestrus-derived EVs are expected to have some effect on blastocyst development, it appeared to be less effective than estrus-derived EVs.

Keywords

Acknowledgement

The authors would like to thank to Seongja Kim for providing the porcine ovaries from the Daejeon Metropolitan Slaughterhouse.

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