Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Analysis of platelet-derived growth factor receptor alpha expression in adult mouse testis

  • Min Seok Woo (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Eun-Jin Kim (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Dong Kun Lee (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Chung Eun Lee (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Gyeongsang National University Hospital, Gyeongsang National University) ;
  • Eun-A Ko (Department of Physiology, Colledge of Medicine, Jeju National University) ;
  • Dawon Kang (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University)
  • Received : 2024.05.03
  • Accepted : 2024.05.07
  • Published : 2024.06.30
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Abstract

Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα's expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes.

Keywords

Acknowledgement

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (2021R1I1A3044128 to Dawon Kang and 2022R1F1A1062897 to Eun-A Ko).

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