한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제37권3호
  • /
  • Pages.247-255
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  • 2024
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지
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Rosa acicularis Leaves Exert Anti-Obesity Activity through AMPK-Dependent Lipolysis and Thermogenesis in Mouse Adipocytes, 3T3-L1 Cells

  • Jeong Won Choi (Department of Forest Science, Andong National University) ;
  • Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
  • Jin Boo Jeong (Department of Forest Science, Andong National University)
  • 투고 : 2024.01.29
  • 심사 : 2024.02.13
  • 발행 : 2024.06.01
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초록

In this study, we aimed to verify the anti-obesity activity of R. acicularis leaves (RAL) and elucidate its mechanism of action in 3T3-L1 preadipocytes. RAL dose-dependently inhibited the accumulation of lipid droplets and triacylglycerol. RAL did not affect cell proliferation and survival in undifferentiated 3T3-L1 cells, but it inhibited cell proliferation in differentiating 3T3-L1 cells. RAL increased ATGL, p-HSL, and HSL, and decreased perilipin-1 in differentiating 3T3-L1 cells. In addition, RAL reduced lipid droplet accumulation and increased free glycerol content in differentiated 3T3-L1 cells. RAL increased ATGL and HSL in differentiated 3T3-L1 cells. Also, RAL increased p-AMPK, PPARγ, UCP-1, and PGC-1α in differentiating 3T3-L1 cells. AMPK inhibition by compound C attenuated RAL-mediated increase of ATGL, HSL, PPARγ, and UCP-1 in 3T3-L1 cells. Taken together, it is thought that RAL may inhibit lipid accumulation through lipolysis and thermogenesis via the activation of AMPK in adipocytes.

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과제정보

This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) and funded by the Ministry of Education (NRF-2018R1A6A1A03024862).

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