Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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Fertilizing capacity of cryopreserved sperm of Chirostoma jordani (Woolman, 1894)

  • Bustamante-Gonzalez Jesus Damaso (Planta Experimental de Produccion Acuicola, Universidad Autonoma Metropolitana-Iztapalapa (UAM-I)) ;
  • Gutierrez-Diaz Dulce Leticia (Maestria en Ciencias Agropecuarias, Universidad Autonoma Metropolitana-Xochimilco (UAM-X)) ;
  • Baca-Alejo Judith Sarai (Escuela Superior de Medicina, Instituto Politecnico Nacional (IPN)) ;
  • Figueroa-Lucero Gerardo (Planta Experimental de Produccion Acuicola, Universidad Autonoma Metropolitana-Iztapalapa (UAM-I)) ;
  • Arenas-Rios Edith (Laboratorio de Morfofisiologia y Bioquimica del Espermatozoide, UAM-I) ;
  • Hernandez-Rubio Maria Cecilia (Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, IPN) ;
  • Avalos-Rodriguez Alejandro (Laboratorio de Bioquimica de la Reproduccion, UAM-X)
  • 투고 : 2023.10.20
  • 심사 : 2024.01.26
  • 발행 : 2024.05.31
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초록

The genus Chirostoma is endemic from the Mesa Central of Mexico. It is conformed of 18 species and six subspecies. Five species are in some category of risk, because of this, Chirostoma jordani is an excellent model species to implement biotechnologies like gametes cryopreservation. Aim of present study was to evaluate fertilizing capacity of cryopreserved C. jordani sperm, as alternative to conservation and assisted reproduction in this specie and genus. Males and females were collected from wild Atlangatepec dam stock, Tlaxcala State, Mexico. Seminal quality was evaluated in fresh and cryopreserved semen with three cryoprotective agents (CPAs): 10% dimethyl sulfoxide (DMSO), 10% methanol (MeOH), 14% ethylene glycol (EG) and it was determined its post-thaw fertilizing capacity. Sperm motility percentage decreased during cryopreservation process (p < 0.05). There were not significant differences in post-thaw motility percentage between EG (53.5 ± 1.9%) and MeOH (53.3 ± 1.3%), but DMSO (50.3 ± 0.5%) was significantly different (p < 0.05). Results showed that 0.2 μL fresh semen were enough to fertilize 100% oocytes (n = 60). 10 μL DMSO and 5 μL MeOH and EG cryopreserved semen were necessary to fertilize oocytes 100% (n = 60) (p < 0.05). Cryopreservation and fertilization protocol for C. jordani sperm was efficient and it could be used for its assisted reproduction.

키워드

과제정보

Present research is part of posdoctoral stay of first author (BGJD, CVU: 566816).

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