Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Correlation between Rab3A Expression and Sperm Kinematic Characteristics

  • Seung-Ik Jang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jae-Hwan Jo (Department of Animal Biotechnology, Kyungpook National University) ;
  • Uwamahoro Claudine (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Eun-Ju Jung (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Woo-Jin Lee (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Ju-Mi Hwang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jeong-Won Bae (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Dae-Hyun Kim (Department of Animal Science, Chonnam National University) ;
  • Jun Koo Yi (School of Animal Life Convergence Science, Hankyong National University) ;
  • Jae Jung Ha (Gyeongbuk Livestock Research Institute) ;
  • Dong Yep Oh (Gyeongbuk Livestock Research Institute) ;
  • Woo-Sung Kwon (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Received : 2024.01.04
  • Accepted : 2024.02.22
  • Published : 2024.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Ras-related (Rab) proteins, integral members of the monomeric G-protein family, play a pivotal role in regulating intracellular vesicular transport. These proteins contribute to male reproductive processes, specifically in acrosome formation, exocytosis, and sperm motility. Although a prior study indicated a correlation between Rab3A and sperm motility, including motion kinematic parameters such as mean dance, this association has only been explored within a limited sample size. Therefore, further verification is required to confirm the correlation between Rab3A and sperm motility parameters. In the present study, Rab3A expression, sperm motility, and motion kinematic parameters were analyzed in 150 boar spermatozoa. Additionally, correlations between Rab3A expression and sperm kinematic characteristics were evaluated statistically. The results revealed significant associations between Rab3A protein expression levels and various motion kinematic parameters. Specifically, Rab3A levels exhibited positive correlations with average path velocity (p<0.05), mean amplitude of lateral head displacement (p<0.05), and curvilinear velocity (p<0.01). Consequently, it is proposed that Rab3A protein plays a crucial role in male fertility through its correlation with sperm kinematic characteristics, making it a potential marker for sperm motility-related assessments.

Keywords

References

  1. Aghazarian A, Huf W, Pfluger H, Klatte T (2021) Standard semen parameters vs. sperm kinematics to predict sperm DNA damage. World J Mens Health 39:116-122.
  2. Alory C, Balch WE (2001) Organization of the Rab-GDI/CHM superfamily: The functional basis for choroideremia disease. Traffic 2:532-543.
  3. Bae JW, Hwang JM, Kwon WS (2022a) Prediction of male fertility using Ras-related proteins. J Anim Sci Technol 64:1024-1034.
  4. Bae JW, Kim SH, Kim DH, Ha JJ, Yi JK, Hwang S, Ryu BY, Pang MG, Kwon WS (2019) Rasrelated proteins (Rab) are key proteins related to male fertility following a unique activation mechanism. Reprod Biol 19:356-362.
  5. Bae JW, Yi JK, Jeong EJ, Lee WJ, Hwang JM, Kim DH, Ha JJ, Kwon WS (2022b) Ras-related proteins (Rab) play significant roles in sperm motility and capacitation status. Reprod Biol 22:100617.
  6. Bongso TA, Ng SC, Mok H, Lim MN, Teo HL, Wong PC, Ratnam SS (1989) Effect of sperm motility on human in vitro fertilization. Arch Androl 22:185-190.
  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
  8. Constantin G, Laudanna C (2010) Leukocyte chemotaxis: From lysosomes to motility. Nat Immunol 11:463-464.
  9. Donnelly ET, Lewis SEM, McNally JA, Thompson W (1998) In vitro fertilization and pregnancy rates: The influence of sperm motility and morphology on IVF outcome. Fertil Steril 70:305-314.
  10. Goody RS, Muller MP, Wu YW (2017) Mechanisms of action of Rab proteins, key regulators of intracellular vesicular transport. Biol Chem 398:565-575.
  11. Goud B, Liu S, Storrie B (2018) Rab proteins as major determinants of the Golgi complex structure. Small GTPases 9:66-75.
  12. Hinrichs K, Loux SC (2012) Hyperactivated sperm motility: Are equine sperm different? J Equine Vet Sci 32:441-444.
  13. Hutagalung AH, Novick PJ (2011) Role of Rab GTPases in membrane traffic and cell physiology. Physiol Rev 91:119-149.
  14. Iida H, Noda M, Kaneko T, Doiguchi M, Mori T (2005) Identification of rab12 as a vesicle-associated small GTPase highly expressed in Sertoli cells of rat testis. Mol Reprod Dev 71:178-185.
  15. Jang SI, Jo JH, Jung EJ, Lee WJ, Hwang JM, Bae JW, Kwon WS (2022) Development of an optimal protocol to induce capacitation of boar spermatozoa in vitro. J Anim Reprod Biotechnol 37:285-291.
  16. Jung EJ, Lee WJ, Hwang JM, Bae JW, Kwon WS (2022) Reproductive toxicity of ritonavir in male: Insight into mouse sperm capacitation. Reprod Toxicol 114:1-6.
  17. Kumar S, Lee HJ, Park HS, Lee K (2016) Testis-specific GTPase (TSG): An oligomeric protein. BMC Genomics 17:792.
  18. Kwon WS, Rahman MS, Ryu DY, Park YJ, Pang MG (2015) Increased male fertility using fertility-related biomarkers. Sci Rep 5:15654.
  19. Lau ASN, Mruk DD (2003) Rab8B GTPase and junction dynamics in the testis. Endocrinology 144:1549-1563.
  20. Lin YH, Ke CC, Wang YY, Chen MF, Chen TM, Ku WC, Chiang HS, Yeh CH (2017) RAB10 interacts with the male germ cell-specific GTPase-activating protein during mammalian spermiogenesis. Int J Mol Sci 18:97.
  21. Liu S, Storrie B (2012) Are Rab proteins the link between Golgi organization and membrane trafficking? Cell Mol Life Sci 69:4093-4106.
  22. Moreno RD, Ramalho-Santos J, Sutovsky P, Chan EKL, Schatten G (2000) Vesicular traffic and Golgi apparatus dynamics during mammalian spermatogenesis: Implications for acrosome architecture. Biol Reprod 63:89-98.
  23. Nakamura Y, Asano A, Hosaka Y, Takeuchi T, Iwanaga T, Yamano Y (2015) Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes. Exp Anim 64:415-424.
  24. Pereira-Leal JB, Seabra MC (2000) The mammalian Rab family of small GTPases: Definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily. J Mol Biol 301:1077-1087.
  25. Ramalho-Santos J, Moreno RD, Wessel GM, Chan EKL, Schatten G (2001) Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis. Exp Cell Res 267:45-60.
  26. Rodriguez-Gil JE, Silvers G, Flores E, Jesus Palomo M, Ramirez A, Montserrat Rivera M, Castro M, Brito M, Bucher D, Correa J, Concha II (2007) Expression of the GM-CSF receptor in ovine spermatozoa: GM-CSF effect on sperm viability and motility of sperm subpopulations after the freezing-thawing process. Theriogenology 67:1359-1370.
  27. Shan MM, Sun SC (2021) The multiple roles of RAB GTPases in female and male meiosis. Hum Reprod Update 27:1013-1029.
  28. Smith CE, Hermo L, Fazel A, Lalli MF, Bergeron JJM (1990) Ultrastructural distribution of NADPase within the Golgi apparatus and lysosomes of mammalian cells. Prog Histochem Cytochem 21:1-120.
  29. Stenmark H (2009) Rab GTPases as coordinators of vesicle traffic. Nat Rev Mol Cell Biol 10:513-525.
  30. Suarez SS, Dai X (1992) Hyperactivation enhances mouse sperm capacity for penetrating viscoelastic media. Biol Reprod 46:686-691.
  31. Vieira OV (2018) Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair. Small GTPases 9:349-351.
  32. Wu YW, Oesterlin LK, Tan KT, Waldmann H, Alexandrov K, Goody RS (2010) Membrane targeting mechanism of Rab GTPases elucidated by semisynthetic protein probes. Nat Chem Biol 6:534-540.
  33. Yunes R, Michaut M, Tomes C, Mayorga LS (2000) Rab3A triggers the acrosome reaction in permeabilized human spermatozoa. Biol Reprod 62:1084-1089.