Anatomy and Cell Biology

  • 제57권1호
  • /
  • Pages.129-142
  • /
  • 2024
  • /
  • 2093-3665(pISSN)
  • /
  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
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Bisphosphonate's effect on the tongue in adult male albino rats and the possible protective role of rutin: light and scanning electron microscopic study

  • Dalia El-sayed El-ghazouly (Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University) ;
  • Rania Ibrahim Yassien (Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University)
  • 투고 : 2023.09.03
  • 심사 : 2023.12.21
  • 발행 : 2024.03.31
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초록

Alendronate sodium (ALS) is a nitrogen-containing bisphosphonate used for the treatment of different bone disorders. However, its adverse effect on oral soft tissue has been detected. Rutin (RUT) is natural flavonoid with antioxidant and anti-inflammatory properties. This work aimed to investigate the possible effect of ALS on the tongue of adult male albino rats and to evaluate the possible protective role of RUT. Forty adult male albino rats were equally divided into four groups: group I (control), group II (RUT): Received RUT 50 mg/kg, group III (ALS): Received ALS 1 mg/kg, group IV (ALS+RUT):(ALS+RUT): Received ALS and RUT with the same doses as pervious groups. The drugs were given once daily for 5 weeks. Tongue specimens were taken and processed for light and scanning electron microscopic inspection. ALS treated group revealed structural changes in the tongue in the form of decrease in the height of the filiform papillae with blunt ends, marked atrophy in some papillae with areas of focal loss, loss of some epithelial cells, pyknotic nuclei and cytoplasmic vacuoles in some epithelial cells. The lamina propria showed inflammatory cellular infiltration with congested blood vessels. Statistically, there were highly significant decrease in the number of proliferating cell nuclear antigen immunopositive cells, area percentage of Bcl-2 immunoexpression and highly significant increase in the collagen content compared to control group. Administration of RUT with ALS minimizes these changes. RUT protected the rat tongue against the histological and immunohistochemical changes induced by ALS through its antioxidant and anti-inflammatory properties.

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참고문헌

  1. Zandi M, Dehghan A, Talimkhani I, Rezaeian L, Mohammad Gholi Mezerji N. Histological evaluation of the healing process of autografted mandibular bone defects in rats under treatment with zoledronate. J Craniomaxillofac Surg 2019;47:1779-86. 
  2. Oyhanart SR, Escudero ND, Mandalunis PM. Effect of alendronate on the mandible and long bones: an experimental study in vivo. Pediatr Res 2015;78:618-25. 
  3. Psimma C, Psimma Z, Willems HC, Kluter WJ, van der Maarel-Wierink CD. Oral bisphosphonates: adverse effects on the oral mucosa not related to the jaw bones. A scoping review. Gerodontology 2022;39:330-8. 
  4. Farag DB, Mehanny SS. Histopathological alterations of the intrinsic tongue muscles following zoledronic acid treatment in a rat model. Dent Med Probl 2020;57:131-6. 
  5. Badae NM, Ghazala RA, Zaki EI, Abdel-Ghani SA. Evaluating the therapeutic effect of Diallyl disulfide compared to that of Alderonate on Glucocorticoids induced osteoporosis in rats: Biochemical and histomorphometric analysis. Bull Egypt Soc Physiol Sci 2019;39:129-42. 
  6. Tokmak Ozsahin ET, Cam B, Dere F, Kurkcu M, Evruke C, Soames R, Oguz O. The effect of alendronate sodium on trabecular bone structure in an osteoporotic rat model. Turk J Phys Med Rehabil 2017;63:165-73. 
  7. Papamitsou T, Morsi-Yeroyannis A, Papanastasiou A, Bakalopoulos N, Dietrich EM, Karachrysafi S, Toskas A, Mareti E, Morsi-Yeroyanni A, Sioga A. Bisphosphonate's effect on tongue mucosa: an experimental electron microscopy study. Medicina (Kaunas) 2020;56:51. 
  8. Donetti E, Gualerzi A, Sardella A, Lodi G, Carrassi A, Sforza C. Alendronate impairs epithelial adhesion, differentiation and proliferation in human oral mucosa. Oral Dis 2014;20:466-72. 
  9. Theodora P, Stella F, Angeliki P, Eva-Maria D, Dimitris K, Alexandros T, Sofia K, Antonia S. Effect of alendronic acid on buccal mucosa. J Dent Oral Health 2018;4:1-6. 
  10. Erdogan E, Ilgaz Y, Gurgor PN, Oztas Y, Topal T, Oztas E. Rutin ameliorates methotrexate induced hepatic injury in rats. Acta Cir Bras 2015;30:778-84. 
  11. Al-Hamadawi HA, Al-Ankoshy AAM, Alqershi KA. Studying the protective and therapeutic role of Rutin on the histological structure of the liver and some physiological parameters in Rats treated with ciprofloxacin. BNIHS 2022;140:1113-22. 
  12. Hussani MF, Sabour AN. Effect of rutin on the histological structure of the heart and some biochemical indicators of oxidative stress in male Albino rats. PJMHS 2022;16:653-7. 
  13. Thandavamoorthy P, Balan R, Subramaniyan J, Arumugam M, John B, Krishnan G, Ramasamy E, Mani GK, Rajendran R, Thiruvengadam D. Alleviative role of rutin against 4-Nitroquinoline-1-oxide (4-NQO) provoked oral squamous cell carcinoma in experimental animal model. J Pharm Res 2014;8:899-906. 
  14. Motamedshariaty VS, Amel Farzad S, Nassiri-Asl M, Hosseinzadeh H. Effects of rutin on acrylamide-induced neurotoxicity. Daru 2014;22:27. 
  15. Gaertner D, Hallman T, Claire Hankenson F, Batchelder MA. Anesthesia and analgesia for laboratory rodents. In: Fish RE, Brown MJ, Danneman PJ, Karas AZ, editors. Anesthesia and Analgesia in Laboratory Animals. 2nd ed. Academic Press; 2008. p.239-97. 
  16. Kiernan JA. Histological and histochemical methods: theory and practice. 5th ed. Scion; 2015. p. 238-310. 
  17. Ramos-Vara JA, Kiupel M, Baszler T, Bliven L, Brodersen B, Chelack B, Czub S, Del Piero F, Dial S, Ehrhart EJ, Graham T, Manning L, Paulsen D, Valli VE, West K. Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. J Vet Diagn Invest 2008;20:393-413. 
  18. Ozer H, Yenicesu G, Arici S, Cetin M, Tuncer E, Cetin A. Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors. Diagn Pathol 2012;7:124. 
  19. Piroeva I, Atanassova-Vladimirova S, Dimowa L, Sbirkova H, Radoslavov G, Hristov P, Shivachev BL. A simple and rapid scanning electron microscope preparative technique for observation of biological samples: application on bacteria and DNA samples. Bulgarian Chem Commun 2013;45:510-5. 
  20. White SE, Malley J, Carton L, Dawson B. Basic and clinical biostatistics. 5th ed. McGraw-Hill Companies; 2020. 
  21. Fideles LS, de Miranda JAL, Martins CDS, Barbosa MLL, Pimenta HB, Pimentel PVS, Teixeira CS, Scafuri MAS, Facanha SO, Barreto JEF, Carvalho PMM, Scafuri AG, Araujo JL, Rocha JA, Vieira IGP, Ricardo NMPS, da Silva Campelo M, Ribeiro MENP, de Castro Brito GA, Cerqueira GS. Role of Rutin in 5-fluorouracil-induced intestinal mucositis: prevention of histological damage and reduction of inflammation and oxidative stress. Molecules 2020;25:2786. 
  22. Kharazmi M, Sjoqvist K, Warfvinge G. Oral ulcers, a little known adverse effect of alendronate: review of the literature. J Oral Maxillofac Surg 2012;70:830-6. 
  23. Carvalho NS, Silva MM, Silva RO, Nicolau LA, Araujo TS, Costa DS, Sousa NA, Souza LK, Soares PM, Medeiros JV. Protective effects of simvastatin against alendronate-induced gastric mucosal injury in rats. Dig Dis Sci 2016;61:400-9. 
  24. Kassab AA. Effect of Fosamax on the duodenal mucosa in adult male albino rats and the possible protection by nigella sativa oil: a histological and immunohistochemical study. Egypt J Histol 2019;42:900-14. 
  25. Kassab AA, Moustafa KA, Abd-El-Hafez AA. The possible protective role of pumpkin seed oil in ameliorating tongue mucosal damage induced by orlistat in adult male albino rats: a light and scanning electron microscopic study. Egypt J Histol 2020;43:975-87. 
  26. Pourgonabadi S, Ghorbani A, Tayarani Najarn Z, Mousavi SH. In vitro assessment of alendronate toxic and apoptotic effects on human dental pulp stem cells. Iran J Basic Med Sci 2018;21:905-10. 
  27. Saso L, Suzen S, Borges F, Csont T. Chemistry and pharmacology of modulators of oxidative stress. Curr Med Chem 2020;27:2038-9. 
  28. Thoma A, Lightfoot AP. NF-kB and inflammatory cytokine signalling: role in skeletal muscle atrophy. Adv Exp Med Biol 2018;1088:267-79. 
  29. Tian C, Liu X, Chang Y, Wang R, Yang M, Liu M. Rutin prevents inflammation induced by lipopolysaccharide in RAW 264.7 cells via conquering the TLR4-MyD88-TRAF6-NF-κB signalling pathway. J Pharm Pharmacol 2021;73:110-7. 
  30. Li Y, Qin L, Ying L, Dong H, Wang D. Rutin prevents retinal ganglion cell death and exerts protective effects by regulating transforming growth factor-β2/Smad2/3Akt/PTEN signaling in experimental rat glaucoma. Trop J Pharm Res 2019;18:985-93. 
  31. Bai L, Li A, Gong C, Ning X, Wang Z. Protective effect of rutin against bleomycin induced lung fibrosis: Involvement of TGF-β1/α-SMA/Col I and III pathway. Biofactors 2020;46:637-44.