Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

  • Darwish Badran (Department of Anatomy and Histology, Faculty of Medicine, the University of Jordan and Ibn Sina University for Medical Sciences) ;
  • Ayman El-Baz El-Agroudy (Department of Basic Medical Sciences, Faculty of Medicine, Ibn Sina University for Medical Sciences) ;
  • Amira Adly Kassab (Department of Basic Medical Sciences, Faculty of Medicine, Ibn Sina University for Medical Sciences) ;
  • Khaled Saad El-Bayoumi (Department of Basic Medical Sciences, Faculty of Medicine, Ibn Sina University for Medical Sciences) ;
  • Zienab Helmy Eldken (Department of Basic Medical Sciences, Faculty of Medicine, Ibn Sina University for Medical Sciences) ;
  • Noha Ramadan Mohammed Elswaidy (Department of Histology and Cell Biology, Faculty of Medicine, Tanta University)
  • Received : 2023.08.25
  • Accepted : 2023.10.31
  • Published : 2024.03.31
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Abstract

The world has witnessed tremendous advancements in nano-base applications. Zinc oxide nanoparticles (ZON) are widely used in food industry and medicine. Although their application is of important value, they may cause toxicity to body tissues. Peripheral blood mononuclear cells (PBMCs) proved its efficacy in tissue regeneration especially when it is preconditioned by activated platelet supernatant (APS). The aim of this study is to evaluate the effect of ZON on the gastric mucosa and the therapeutic role of the PBMCs preconditioned by APS in rats. Ten rats were donors and fifty rats were recipients. The recipients were divided into; control group, ZON group (10 mg/kg/day orally for five days) and preconditioned PBMCs group (1×107 once intravenously 24 hours after ZON). Gastric specimens were processed for histological, immunohistochemical, biochemical and quantitative real-time polymerase chain reaction studies. ZON group showed marked structural changes in the gastric mucosa. There was desquamation or deep ulceration of the epithelium. Cytoplasmic vacuoles and pyknotic nuclei were in glandular cells. Reduced proliferating cell nuclear antigen and increased tumor necrosis factor-α were in epithelial cells. There were significant elevation in malondialdahyde and reduction in glutathione, superoxide dismutase, and catalase. Enhancement in mRNA expression of nuclear factor kappa-B and cyclooxygenase-2 was detected. The preconditioned PBMCs group showed significant improvement of all parameters. So, ZON had cytotoxic effects on the gastric mucosa and the preconditioned PBMCs had a therapeutic effect on gastric mucosal damage after ZON.

Keywords

References

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