Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Feeding, excretion, survival, and histological alterations in zebrafish Danio rerio from single and combined exposure to microplastics and copper

  • Hyeon Jin Kim (Department of Aqualife Medicine, Chonnam National University) ;
  • So Ryung Shin (Department of Aqualife Medicine, Chonnam National University) ;
  • Jung Jun Park (Aquaculture Industry Research Division, East Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Jung Sick Lee (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2023.11.29
  • Accepted : 2024.02.08
  • Published : 2024.03.31
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Abstract

This study evaluated the risk of single and combined exposure to microplastics in zebrafish (Danio rerio) through biomarkers, such as survival rate, excretion rate, and histological alterations of organ systems. The experimental groups were the control(Cont.), single microplastics exposure group(MPs, 1.83%/fish total weight(g)), the copper group(Cu, 21.6 ㎍ L-1), and a group with combined exposure to MPs and copper (MPs*Cu). The experiment was conducted with individual exposure (7 days) for MP excretion rate analysis and group exposure (14 days) for biomarker analysis. The daily excretion rate of MPs tended to decrease in a time-dependent manner. The copper concentration in the body was not significantly different between single and combined copper exposure. The degeneration of mucous cells in the skin, capillary dilation of the gill lamella, increased intestinal mucous, hepatocyte hypertrophy, and the degeneration of glomeruli and renal tubules were observed in all exposure groups. These histological alterations showed the highest tendency in the MPs*Cu group. In this study, the changes in biomarkers were attributed to the single effect of copper or the combined effect of copper and MPs rather than being solely influenced by MPs.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Fisheries Science (R2024046) of South Korea.

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