Nutrition Research and Practice

  • 제18권4호
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  • Pages.479-497
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  • 2024
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  • 1976-1457(pISSN)
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  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
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Cryptotanshinone promotes brown fat activity by AMPK activation to inhibit obesity

  • Jie Ni (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University) ;
  • Aili Ye (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University) ;
  • Liya Gong (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University) ;
  • Xiafei Zhao (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University) ;
  • Sisi Fu (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University) ;
  • Jieya Guo (Department of Endocrinology, Affiliated Xiaoshan Hospital, Hangzhou Normal University)
  • 투고 : 2024.03.11
  • 심사 : 2024.05.02
  • 발행 : 2024.08.01
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초록

BACKGROUND/OBJECTIVES: Activating brown adipose tissue (BAT) and browning of white adipose tissue (WAT) can protect against obesity and obesity-related metabolic conditions. Cryptotanshinone (CT) regulates lipid metabolism and significantly ameliorates insulin resistance. Adenosine-5'-monophosphate (AMP)-activated protein kinase (AMPK), a receptor for cellular energy metabolism, is believed to regulate brown fat activity in humans. MATERIALS/METHODS: The in vivo study included high-fat-fed obese mice administered orally 200/400 mg/kg/d CT. They were evaluated through weight measurement, the intraperitoneal glucose tolerance test (IPGTT), intraperitoneal insulin tolerance test (IPITT), cold stimulation test, serum lipid (total cholesterol, triglycerides, and low-density lipoprotein) measurement, hematoxylin and eosin staining, and immunohistochemistry. Furthermore, the in vitro study investigated primary adipose mesenchymal stem cells (MSCs) with incubation of CT and AMPK agonists (acadesine)/inhibitor (Compound C). Cells were evaluated using Oil Red O staining, Alizarin red staining, flow cytometry, and immunofluorescence staining to identify and observe the osteogenic versus adipogenic differentiation. Quantitative real-time polymerase chain reaction and the Western blot were used to observe related gene expression. RESULTS: In the diet-induced obesity mouse model mice CT suppressed body weight, food intake, glucose levels in the IPGTT and IPTT, serum lipids, the volume of adipose tissue, and increased thermogenesis, uncoupling protein 1, and the AMPK pathway expression. In the in vitro study, CT prevented the formation of lipid droplets from MSCs while activating brown genes and the AMPK pathway. AMPK activator enhanced CT's effects, while the AMPK inhibitor reversed the effects of CT. CONCLUSION: CT promotes adipose tissue browning to increase body thermogenesis and reduce obesity by activating the AMPK pathway. This study provides an experimental foundation for the use of CT in obesity treatment.

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과제정보

This work was supported by the Scientific Research Project of Agriculture and Social Development in Hangzhou (20211231Y128).

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