The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

  • Khaerani Nurlaelita (Phytopathology, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Arif Wibowo (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Ani Widiastuti (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada)
  • 투고 : 2024.04.09
  • 심사 : 2024.06.10
  • 발행 : 2024.08.01
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초록

Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

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과제정보

This study has been funded by the research grant of the Indonesian Endowment Fund for Education (LPDP)- Ministry of Finance, Indonesia. We thank Dr. Adyatma Wirawan Santosa, S.P., M.Sc. for technical support in phylogenetic tree analysis and Sri Giyanti for technical support in the laboratory. This report is a part of master thesis research conducted by the first author, under supervision corresponding and third author.

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