The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.)

  • Ji-Soo Park (Department of Plant Medicine, Division of Bio-Resource Sciences, College of Agricultural and Life Sciences, Kangwon National University) ;
  • Dong-Joo Min (Department of Plant Medicine, Division of Bio-Resource Sciences, College of Agricultural and Life Sciences, Kangwon National University) ;
  • Tae-Seon Park (Department of Plant Medicine, Division of Bio-Resource Sciences, College of Agricultural and Life Sciences, Kangwon National University) ;
  • You-Seop Shin (Department of Plant Medicine, Division of Bio-Resource Sciences, College of Agricultural and Life Sciences, Kangwon National University) ;
  • Jin-Sung Hong (Department of Plant Medicine, Division of Bio-Resource Sciences, College of Agricultural and Life Sciences, Kangwon National University)
  • 투고 : 2024.06.09
  • 심사 : 2024.07.11
  • 발행 : 2024.08.01
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초록

The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.

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과제정보

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of the technique to predict changes of diverse plant viral disease caused by climate change using the pathogen-crop virome study, Project No. PJ14983)" Rural Development Administration, Republic of Korea.

참고문헌

  1. Baek, H.-S., Na, Y.-S., Kim, D.-H., Lee, C.-H., Ryu, B.-H. and Song, S. K. 2004. Antioxidant activities of Stachys sieboldii MIQ roots. J. Life Sci. 14:1-7 (in Korean). 
  2. Fuji, S., Yamamoto, H., Furuya, H. and Naito, H. 2003. Characterization of a new potyvirus isolated from Chinese artichoke in Japan. Arch. Virol. 148:2249-2255. 
  3. Ha, C., Coombs, S., Revill, P. A., Harding, R. M., Vu, M. and Dale, J. L. 2008. Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Arch. Virol. 153:25-36. 
  4. Ikemoto, M., Yamazaki, A., Ohnishi, T., Ishigami, T., Fukumitsu, J., Oike, K., Inoue, K., Uegaki, K. and Hosokawa, M. 2024. Evaluation of yield and tuber functionality in short-term cultivation of Chinese artichoke using propagated cuttings. J. Hortic. Sci. Biotechnol. 99:480-489. 
  5. Kang, J.-R., Kang, M.-J., Byun, H.-W., Park, J.-H., Kim, D.-I., Chung, S.-Y. and Shin, J.-H. 2018. Effects of sunsik added Stachys sieboldii Miq. on lipid improvement in rats fed a high fat-cholesterol diet. J. Korean Soc. Food Sci. Nutr. 47:657- 663 (in Korean). 
  6. Kil, E.-J., Kim, S., Lee, Y.-J., Byun, H.-S., Park, J., Seo, H., Kim, C.-S., Shim, J.-K., Lee, J.-H., Kim, J.-K., Lee, K.-Y., Choi, H.-S. and Lee, S. 2016. Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes. Sci. Rep. 6:19013. 
  7. Kim, E.-K., You, J.-W., Park, J.-S., Min, D.-J., Park, S.-H. and Hong, J.-S. 2018. Detection and identification of a mixed infection of three viruses in Chinese artichoke in Korea. Res. Plant Dis. 24:81-85 (in Korean). 
  8. Kim, J., Kil, E.-J., Kim, S., Seo, H., Byun, H.-S., Park, J., Chung, M.-N., Kwak, H. R., Kim, M.-K., Kim, C.-S., Yang, J.-W., Lee, K.-Y., Choi, H.-S. and Lee, S. 2015. Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas). Plant Pathol. 64:1284-1291. 
  9. Lee, J. W. and Lim, S. Y. 2018. Comparison of flavonoid content and antioxidant effect of extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. J. Life Sci. 28:841-848 (in Korean). 
  10. Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R. and Li, F. 2018. Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers. J. Virol. Methods 259:122-128. 
  11. Livak, K. J. and Schmittgen, T. D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25:402-408. 
  12. Mascia, T. and Gallitelli, D. 2016. Synergies and antagonisms in virus interactions. Plant Sci. 252:176-192. 
  13. Moradi, Z. and Mehrvar, M. 2021. Whole-genome characterization of alfalfa mosaic virus obtained from metagenomic analysis of Vinca minor and Wisteria sinensis in Iran: with implications for the genetic structure of the virus. Plant Pathol. J. 37:619-631. 
  14. Mueller, E. E. and Grau, C. R. 2007. Seasonal progression, symptom development, and yield effects of Alfalfa mosaic virus epidemics on soybean in Wisconsin. Plant Dis. 91:266-272. 
  15. Murphy, J. F. and Bowen, K. L. 2006. Synergistic disease in pepper caused by the mixed infection of Cucumber mosaic virus and Pepper mottle virus. Phytopathology 96:240-247. 
  16. Ng, J. C. K. and Perry, K. L. 2004. Transmission of plant viruses by aphid vectors. Mol. Plant Pathol. 5:505-511. 
  17. Phan, M. S. V., Seo, J.-K., Choi, H.-S., Lee, S.-H. and Kim, K.-H. 2014. Molecular and biological characterization of an isolate of Cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones. Plant Pathol. J. 30:159-167. 
  18. Revers, F. and Garcia, J. A. 2015. Molecular biology of potyviruses. Adv. Virus Res. 92:101-199. 
  19. Singhal, P., Nabi, S. U., Yadav, M. K. and Dubey, A. 2021. Mixed infection of plant viruses: diagnostics, interactions and impact on host. J. Plant Dis. Prot. 128:353-368.