The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Chamaeneron angustifolia Extract on Lipid Metabolism and Differentiation of 3T3-L1 Preadipocyte

Chamaeneron angustifolia 추출물이 3T3-L1 Preadipocyte의 지방대사 및 분화에 미치는 영향

  • Seul Bi Lee (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Moon-Yeol Choi (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Mi Hyung Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • So-Young Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Mi Ryeo Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University)
  • 이슬비 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최문열 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미형 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김소영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2024.06.10
  • Accepted : 2024.07.25
  • Published : 2024.07.30
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Abstract

Objectives : Obesity is an imbalance between energy intake and consumption due to overeating and lack of exercise, and if it persists, it increases non-infectious diseases such as high blood pressure, diabetes, and hyperlipidemia. In this study, we tried to investigate the possibility of using Chamaeneron angustifolia (CA) as a material for anti-obesity by confirming the effect of inhibiting lipid differentiation. Methods : We measured the effects of CA extract on oil-red-o staining, cell cytotoxicity evaluation activity using 3T3-L1 cells. Additionally, we assessed fat decomposition and metabolism-related protein expression through Western blot analysis. Results : In this study, the anti-obesity effects of CA extract were experimentally assessed. Results showed significant inhibition of adipocyte differentiation and accumulation at concentrations of 0.05, 0.1, and 0.2 mg/ml of oil-red-o staining, with reductions of 80% or more. CA notably increased the phosphorylation of AMPK protein expression compared to the control group across all concentrations. Additionally, phosphorylation of ACC significantly increased at a concentration of 0.2 mg/ml compared to the control. PPAR-γ, which regulates adipogenesis, exhibited a significant decrease compared to the control, while protein expression of CPT-1, involved in fatty acid oxidation, showed a concentration-dependent increase across all groups. Therefore, CA extract demonstrates potential as a functional material for anti-obesity by increasing the expression of proteins related to fat decomposition and synthesis while decreasing others. Conclusions : These results suggest that CA may also be useful as an anti-obesity functional substance.

Keywords

Acknowledgement

이 논문은 농촌진흥청(PJ015272032021)과 한국연구재단(2021R1A2C201471711)의 지원을 받아 수행된 연구입니다.

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