Mass Spectrometry Letters

  • 제15권2호
  • /
  • Pages.69 -78
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  • 2024
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  • 2233-4203(pISSN)
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  • 2093-8950(eISSN)
한국질량분석학회 (Korean Society for Mass Spectrometry)
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Discovery to Human Disease Research: Proteo-Metabolomics Analysis

  • Minjoong Joo (Basilbiotech) ;
  • Jeong-Hun Mok (Department of Medical Device Management and Research, SAIHST, Sungkyunkwan University) ;
  • Van-An Duong (College of Pharmacy, Gachon University) ;
  • Jong-Moon Park (Basilbiotech) ;
  • Hookeun Lee (College of Pharmacy, Gachon University)
  • 투고 : 2024.02.29
  • 심사 : 2024.04.04
  • 발행 : 2024.06.30
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⟨ 이전 논문 다음 논문 ⟩

초록

The advancement of high-throughput omics technologies and systems biology is essential for understanding complex biological mechanisms and diseases. The integration of proteomics and metabolomics provides comprehensive insights into cellular functions and disease pathology, driven by developments in mass spectrometry (MS) technologies, including electrospray ionization (ESI). These advancements are crucial for interpreting biological systems effectively. However, integrating these technologies poses challenges. Compared to genomic, proteomics and metabolomics have limitations in throughput, and data integration. This review examines developments in MS equipped electrospray ionization (ESI), and their importance in the effective interpretation of biological mechanisms. The review also discusses developments in sample preparation, such as Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX), analytical techniques, and data analysis, highlighting the application of these technologies in the study of cancer or Huntington's disease, underscoring the potential for personalized medicine and diagnostic accuracy. Efforts by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) and integrative data analysis methods such as O2PLS and OnPLS extract statistical similarities between metabolomic and proteomic data. System modeling techniques that mathematically explain and predict system responses are also covered. This practical application also shows significant improvements in cancer research, diagnostic accuracy and therapeutic targeting for diseases like pancreatic ductal adenocarcinoma, non-small cell lung cancer, and Huntington's disease. These approaches enable researchers to develop standardized protocols, and interoperable software and databases, expanding multi-omics research application in clinical practice.

키워드

과제정보

This work was a grant from the National Research Foundation (NRF-2022M3H9A2086450) funded by the Korean Ministry of Science, National Research Foundation of Korea (NRF) (No. 2017M3D9A1073784), and ICT (MSIT) and Korea Evaluation Institute Of Industrial Technology (KEIT) (No. 20018578).

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