International Journal of Stem Cells

  • 제17권1호
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  • Pages.38-50
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  • 2024
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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Unleashing the Power of Undifferentiated Induced Pluripotent Stem Cell Bioprinting: Current Progress and Future Prospects

  • Boyoung Kim (Department of Biopharmaceutical Convergence, Sungkyunkwan University) ;
  • Jiyoon Kim (Department of Biopharmaceutical Convergence, Sungkyunkwan University) ;
  • Soah Lee (Department of Biopharmaceutical Convergence, Sungkyunkwan University)
  • 투고 : 2023.09.03
  • 심사 : 2023.11.21
  • 발행 : 2024.02.28
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초록

Induced pluripotent stem cell (iPSC) technology has revolutionized various fields, including stem cell research, disease modeling, and regenerative medicine. The evolution of iPSC-based models has transitioned from conventional two-dimensional systems to more physiologically relevant three-dimensional (3D) models such as spheroids and organoids. Nonetheless, there still remain challenges including limitations in creating complex 3D tissue geometry and structures, the emergence of necrotic core in existing 3D models, and limited scalability and reproducibility. 3D bioprinting has emerged as a revolutionary technology that can facilitate the development of complex 3D tissues and organs with high scalability and reproducibility. This innovative approach has the potential to effectively bridge the gap between conventional iPSC models and complex 3D tissues in vivo. This review focuses on current trends and advancements in the bioprinting of iPSCs. Specifically, it covers the fundamental concepts and techniques of bioprinting and bioink design, reviews recent progress in iPSC bioprinting research with a specific focus on bioprinting undifferentiated iPSCs, and concludes by discussing existing limitations and future prospects.

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과제정보

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE, 2022R1A6A1A03054419), Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT, and Ministry of Health and Welfare (22A0302L1-01, Republic of Korea). The SungKyunKwan University and the BK21 FOUR (Graduate School Innovation) funded by the MOE and NRF.

참고문헌

  1. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-872 https://doi.org/10.1016/j.cell.2007.11.019
  2. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676 https://doi.org/10.1016/j.cell.2006.07.024
  3. Jensen C, Teng Y. Is it time to start transitioning from 2D to 3D cell culture? Front Mol Biosci 2020;7:33
  4. Duval K, Grover H, Han LH, et al. Modeling physiological events in 2D vs. 3D cell culture. Physiology (Bethesda) 2017;32:266-277 https://doi.org/10.1152/physiol.00036.2016
  5. Kim J, Koo BK, Knoblich JA. Human organoids: model systems for human biology and medicine. Nat Rev Mol Cell Biol 2020;21:571-584 https://doi.org/10.1038/s41580-020-0259-3
  6. Hofer M, Lutolf MP. Engineering organoids. Nat Rev Mater 2021;6:402-420 https://doi.org/10.1038/s41578-021-00279-y
  7. Kacarevic ZP, Rider PM, Alkildani S, et al. An introduction to 3D bioprinting: possibilities, challenges and future aspects. Materials (Basel) 2018;11:2199
  8. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147 https://doi.org/10.1126/science.282.5391.1145
  9. Hoffman LM, Carpenter MK. Characterization and culture of human embryonic stem cells. Nat Biotechnol 2005;23:699-708 https://doi.org/10.1038/nbt1102
  10. Yu J, Hu K, Smuga-Otto K, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 2009;324:797-801 https://doi.org/10.1126/science.1172482
  11. Ludwig TE, Levenstein ME, Jones JM, et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 2006;24:185-187 https://doi.org/10.1038/nbt1177
  12. Richards M, Fong CY, Chan WK, Wong PC, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 2002;20:933-936 https://doi.org/10.1038/nbt726
  13. Richards M, Tan S, Fong CY, Biswas A, Chan WK, Bongso A. Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Stem Cells 2003;21:546-556 https://doi.org/10.1634/stemcells.21-5-546
  14. Amit M, Carpenter MK, Inokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 2000;227:271-278 https://doi.org/10.1006/dbio.2000.9912
  15. Xu RH, Peck RM, Li DS, Feng X, Ludwig T, Thomson JA. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2005;2:185-190
  16. Watanabe K, Ueno M, Kamiya D, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 2007;25:681-686 https://doi.org/10.1038/nbt1310
  17. Braam SR, Zeinstra L, Litjens S, et al. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin. Stem Cells 2008;26:2257-2265 https://doi.org/10.1634/stemcells.2008-0291
  18. Miyazaki T, Futaki S, Suemori H, et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat Commun 2012;3:1236
  19. Rodin S, Domogatskaya A, Strom S, et al. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat Biotechnol 2010;28:611-615 https://doi.org/10.1038/nbt.1620
  20. Rodin S, Antonsson L, Hovatta O, Tryggvason K. Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions. Nat Protoc 2014;9:2354-2368 https://doi.org/10.1038/nprot.2014.159
  21. Rodin S, Antonsson L, Niaudet C, et al. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun 2014;5:3195
  22. Olmer R, Haase A, Merkert S, et al. Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium. Stem Cell Res 2010;5:51-64 https://doi.org/10.1016/j.scr.2010.03.005
  23. Steiner D, Khaner H, Cohen M, et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 2010;28:361-364 https://doi.org/10.1038/nbt.1616
  24. Lancaster MA, Renner M, Martin CA, et al. Cerebral organoids model human brain development and microcephaly. Nature 2013;501:373-379 https://doi.org/10.1038/nature12517
  25. Qian X, Nguyen HN, Song MM, et al. Brain-region-specific organoids using mini-bioreactors for modeling ZIKV exposure. Cell 2016;165:1238-1254 https://doi.org/10.1016/j.cell.2016.04.032
  26. Hofbauer P, Jahnel SM, Papai N, et al. Cardioids reveal self-organizing principles of human cardiogenesis. Cell 2021;184:3299-3317.e22 https://doi.org/10.1016/j.cell.2021.04.034
  27. Lewis-Israeli YR, Wasserman AH, Gabalski MA, et al. Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease. Nat Commun 2021;12:5142
  28. Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol 2014;32:773-785 https://doi.org/10.1038/nbt.2958
  29. Arslan-Yildiz A, El Assal R, Chen P, Guven S, Inci F, Demirci U. Towards artificial tissue models: past, present, and future of 3D bioprinting. Biofabrication 2016;8:014103
  30. Holzl K, Lin S, Tytgat L, Van Vlierberghe S, Gu L, Ovsianikov A. Bioink properties before, during and after 3D bioprinting. Biofabrication 2016;8:032002
  31. Khoeini R, Nosrati H, Akbarzadeh A, et al. Natural and synthetic bioinks for 3D bioprinting. Adv NanoBiomed Res 2021;1:2000097
  32. Vijayavenkataraman S, Yan WC, Lu WF, Wang CH, Fuh JYH. 3D bioprinting of tissues and organs for regenerative medicine. Adv Drug Deliv Rev 2018;132:296-332 https://doi.org/10.1016/j.addr.2018.07.004
  33. Guillemot F, Souquet A, Catros S, et al. High-throughput laser printing of cells and biomaterials for tissue engineering. Acta Biomater 2010;6:2494-2500 https://doi.org/10.1016/j.actbio.2009.09.029
  34. Kim JD, Choi JS, Kim BS, Chan Choi Y, Cho YW. Piezoelectric inkjet printing of polymers: stem cell patterning on polymer substrates. Polymer 2010;51:2147-2154 https://doi.org/10.1016/j.polymer.2010.03.038
  35. Chang CC, Boland ED, Williams SK, Hoying JB. Direct-write bioprinting three-dimensional biohybrid systems for future regenerative therapies. J Biomed Mater Res B Appl Biomater 2011;98:160-170 https://doi.org/10.1002/jbm.b.31831
  36. Koch L, Kuhn S, Sorg H, et al. Laser printing of skin cells and human stem cells. Tissue Eng Part C Methods 2010;16:847-854 https://doi.org/10.1089/ten.tec.2009.0397
  37. Michael S, Sorg H, Peck CT, et al. Tissue engineered skin substitutes created by laser-assisted bioprinting form skin-like structures in the dorsal skin fold chamber in mice. PLoS One 2013;8:e57741
  38. Norotte C, Marga FS, Niklason LE, Forgacs G. Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 2009;30:5910-5917 https://doi.org/10.1016/j.biomaterials.2009.06.034
  39. Smith CM, Stone AL, Parkhill RL, et al. Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. Tissue Eng 2004;10:1566-1576 https://doi.org/10.1089/ten.2004.10.1566
  40. Marga F, Jakab K, Khatiwala C, et al. Toward engineering functional organ modules by additive manufacturing. Biofabrication 2012;4:022001
  41. Li X, Liu B, Pei B, et al. Inkjet Bioprinting of Biomaterials. Chem Rev 2020;120:10793-10833 https://doi.org/10.1021/acs.chemrev.0c00008
  42. Xu T, Jin J, Gregory C, Hickman JJ, Boland T. Inkjet printing of viable mammalian cells. Biomaterials 2005;26:93-99 https://doi.org/10.1016/j.biomaterials.2004.04.011
  43. Mirdamadi E, Tashman JW, Shiwarski DJ, Palchesko RN, Feinberg AW. FRESH 3D bioprinting a full-size model of the human heart. ACS Biomater Sci Eng 2020;6:6453-6459 https://doi.org/10.1021/acsbiomaterials.0c01133
  44. Kim E, Choi S, Kang B, et al. Creation of bladder assembloids mimicking tissue regeneration and cancer. Nature 2020;588:664-669 https://doi.org/10.1038/s41586-020-3034-x
  45. Guillotin B, Souquet A, Catros S, et al. Laser assisted bioprinting of engineered tissue with high cell density and microscale organization. Biomaterials 2010;31:7250-7256 https://doi.org/10.1016/j.biomaterials.2010.05.055
  46. Zhu W, Ma X, Gou M, Mei D, Zhang K, Chen S. 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol 2016;40:103-112 https://doi.org/10.1016/j.copbio.2016.03.014
  47. Yu C, Ma X, Zhu W, et al. Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix. Biomaterials 2019;194:1-13 https://doi.org/10.1016/j.biomaterials.2018.12.009
  48. Coffin BD, Hudson AR, Lee A, Feinberg AW. FRESH 3D bioprinting a ventricle-like cardiac construct using human stem cell-derived cardiomyocytes. Methods Mol Biol 2022;2485:71-85 https://doi.org/10.1007/978-1-0716-2261-2_5
  49. Maiullari F, Costantini M, Milan M, et al. A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes. Sci Rep 2018;8:13532
  50. Noor N, Shapira A, Edri R, Gal I, Wertheim L, Dvir T. 3D printing of personalized thick and perfusable cardiac patches and hearts. Adv Sci (Weinh) 2019;6:1900344
  51. Lawlor KT, Vanslambrouck JM, Higgins JW, et al. Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation. Nat Mater 2021;20:260-271 https://doi.org/10.1038/s41563-020-00853-9
  52. Choi K, Park CY, Choi JS, et al. The effect of the mechanical properties of the 3D printed gelatin/hyaluronic acid scaffolds on hMSCs differentiation towards chondrogenesis. Tissue Eng Regen Med 2023;20:593-605 https://doi.org/10.1007/s13770-023-00545-w
  53. Narayanan LK, Huebner P, Fisher MB, Spang JT, Starly B, Shirwaiker RA. 3D-bioprinting of polylactic acid (PLA) nanofiber-alginate hydrogel bioink containing human adipose-derived stem cells. ACS Biomater Sci Eng 2016;2:1732-1742 https://doi.org/10.1021/acsbiomaterials.6b00196
  54. Osidak EO, Karalkin PA, Osidak MS, et al. Viscoll collagen solution as a novel bioink for direct 3D bioprinting. J Mater Sci Mater Med 2019;30:31
  55. Duarte Campos DF, Rohde M, Ross M, et al. Corneal bioprinting utilizing collagen-based bioinks and primary human keratocytes. J Biomed Mater Res A 2019;107:1945-1953 https://doi.org/10.1002/jbm.a.36702
  56. Park JA, Lee HR, Park SY, Jung S. Self-organization of fibroblast-laden 3D collagen microstructures from inkjet-printed cell patterns. Adv Biosyst 2020;4:e1900280
  57. Saljo K, Orrhult LS, Apelgren P, Markstedt K, Kolby L, Gatenholm P. Successful engraftment, vascularization, and In vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue. Bioprinting 2020;17:e00065
  58. Kim MH, Lee YW, Jung WK, Oh J, Nam SY. Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting. J Mech Behav Biomed Mater 2019;98:187-194 https://doi.org/10.1016/j.jmbbm.2019.06.014
  59. Faulkner-Jones A, Fyfe C, Cornelissen DJ, et al. Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. Biofabrication 2015;7:044102
  60. Poldervaart MT, Gremmels H, van Deventer K, et al. Prolonged presence of VEGF promotes vascularization in 3D bioprinted scaffolds with defined architecture. J Control Release 2014;184:58-66 https://doi.org/10.1016/j.jconrel.2014.04.007
  61. Snyder JE, Hamid Q, Wang C, et al. Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip. Biofabrication 2011;3:034112
  62. Berg J, Hiller T, Kissner MS, et al. Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus. Sci Rep 2018;8:13877
  63. Xin S, Chimene D, Garza JE, Gaharwar AK, Alge DL. Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting. Biomater Sci 2019;7:1179-1187 https://doi.org/10.1039/C8BM01286E
  64. Skardal A, Zhang J, Prestwich GD. Bioprinting vessel-like constructs using hyaluronan hydrogels crosslinked with tetrahedral polyethylene glycol tetracrylates. Biomaterials 2010;31:6173-6181 https://doi.org/10.1016/j.biomaterials.2010.04.045
  65. Dubbin K, Tabet A, Heilshorn SC. Quantitative criteria to benchmark new and existing bio-inks for cell compatibility. Biofabrication 2017;9:044102
  66. Borkar T, Goenka V, Jaiswal AK. Application of poly-ε-caprolactone in extrusion-based bioprinting. Bioprinting 2021;21:e00111
  67. Merceron TK, Burt M, Seol YJ, et al. A 3D bioprinted complex structure for engineering the muscle-tendon unit. Biofabrication 2015;7:035003
  68. Duan B, Hockaday LA, Kang KH, Butcher JT. 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res A 2013;101:1255-1264 https://doi.org/10.1002/jbm.a.34420
  69. Pataky K, Braschler T, Negro A, Renaud P, Lutolf MP, Brugger J. Microdrop printing of hydrogel bioinks into 3D tissue-like geometries. Adv Mater 2012;24:391-396 https://doi.org/10.1002/adma.201102800
  70. Huang J, Fu H, Wang Z, et al. BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting. RSC Adv 2016;6:108423-108430 https://doi.org/10.1039/C6RA24231F
  71. Rajabi M, McConnell M, Cabral J, Ali MA. Chitosan hydrogels in 3D printing for biomedical applications. Carbohydr Polym 2021;260:117768
  72. Li Y, Jiang X, Li L, et al. 3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation. Biofabrication 2018;10:044101
  73. Engler AJ, Sen S, Sweeney HL, Discher DE. Matrix elasticity directs stem cell lineage specification. Cell 2006;126:677-689 https://doi.org/10.1016/j.cell.2006.06.044
  74. Engler AJ, Carag-Krieger C, Johnson CP, et al. Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating. J Cell Sci 2008; 121(Pt 22):3794-3802 https://doi.org/10.1242/jcs.029678
  75. Lee S, Stanton AE, Tong X, Yang F. Hydrogels with enhanced protein conjugation efficiency reveal stiffness-induced YAP localization in stem cells depends on biochemical cues. Biomaterials 2019;202:26-34 https://doi.org/10.1016/j.biomaterials.2019.02.021
  76. Chaudhuri O, Cooper-White J, Janmey PA, Mooney DJ, Shenoy VB. Effects of extracellular matrix viscoelasticity on cellular behaviour. Nature 2020;584:535-546 https://doi.org/10.1038/s41586-020-2612-2
  77. Ong CS, Yesantharao P, Huang CY, et al. 3D bioprinting using stem cells. Pediatr Res 2018;83:223-231 https://doi.org/10.1038/pr.2017.252
  78. Zhang YS, Arneri A, Bersini S, et al. Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip. Biomaterials 2016;110:45-59 https://doi.org/10.1016/j.biomaterials.2016.09.003
  79. Billiet T, Gevaert E, De Schryver T, Cornelissen M, Dubruel P. The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 2014;35:49-62 https://doi.org/10.1016/j.biomaterials.2013.09.078
  80. Rutz AL, Hyland KE, Jakus AE, Burghardt WR, Shah RN. A multimaterial bioink method for 3D printing tunable, cell-compatible hydrogels. Adv Mater 2015;27:1607-1614 https://doi.org/10.1002/adma.201405076
  81. Kupfer ME, Lin WH, Ravikumar V, et al. In situ expansion, differentiation, and electromechanical coupling of human cardiac muscle in a 3D bioprinted, chambered organoid. Circ Res 2020;127:207-224 https://doi.org/10.1161/CIRCRESAHA.119.316155
  82. Reid JA, Mollica PA, Johnson GD, Ogle RC, Bruno RD, Sachs PC. Accessible bioprinting: adaptation of a low-cost 3D-printer for precise cell placement and stem cell differentiation. Biofabrication 2016;8:025017
  83. Gu Q, Tomaskovic-Crook E, Wallace GG, Crook JM. 3D bioprinting human induced pluripotent stem cell constructs for in situ cell proliferation and successive multilineage differentiation. Adv Healthc Mater 2017;6:1700175
  84. Nguyen D, Hagg DA, Forsman A, et al. Cartilage tissue engineering by the 3D bioprinting of iPS cells in a nanocellulose/alginate bioink. Sci Rep 2017;7:658
  85. Koch L, Deiwick A, Franke A, et al. Laser bioprinting of human induced pluripotent stem cells-the effect of printing and biomaterials on cell survival, pluripotency, and differentiation. Biofabrication 2018;10:035005
  86. Axpe E, Oyen ML. Applications of alginate-based bioinks in 3D bioprinting. Int J Mol Sci 2016;17:1976
  87. Huang G, Li F, Zhao X, et al. Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment. Chem Rev 2017;117:12764-12850 https://doi.org/10.1021/acs.chemrev.7b00094
  88. Handorf AM, Zhou Y, Halanski MA, Li WJ. Tissue stiffness dictates development, homeostasis, and disease progression. Organogenesis 2015;11:1-15 https://doi.org/10.1080/15476278.2015.1019687
  89. Guimaraes CF, Gasperini L, Marques AP, Reis RL. The stiffness of living tissues and its implications for tissue engineering. Nat Rev Mater 2020;5:351-370 https://doi.org/10.1038/s41578-019-0169-1
  90. Pettikiriarachchi JTS, Parish CL, Shoichet MS, Forsythe JS, Nisbet DR. Biomaterials for brain tissue engineering. Aust J Chem 2010;63:1143-1154 https://doi.org/10.1071/CH10159
  91. Rauti R, Renous N, Maoz BM. Mimicking the brain extracellular matrix in vitro: a review of current methodologies and challenges. Israel J Chem 2020;60:1141-1151 https://doi.org/10.1002/ijch.201900052
  92. Novak U, Kaye AH. Extracellular matrix and the brain: components and function. J Clin Neurosci 2000;7:280-290 https://doi.org/10.1054/jocn.1999.0212
  93. Bedossa P, Paradis V. Liver extracellular matrix in health and disease. J Pathol 2003;200:504-515 https://doi.org/10.1002/path.1397
  94. Jain E, Damania A, Kumar A. Biomaterials for liver tissue engineering. Hepatol Int 2014;8:185-197 https://doi.org/10.1007/s12072-013-9503-7
  95. Balestrini JL, Niklason LE. Extracellular matrix as a driver for lung regeneration. Ann Biomed Eng 2015;43:568-576 https://doi.org/10.1007/s10439-014-1167-5
  96. Tebyanian H, Karami A, Nourani MR, et al. Lung tissue engineering: an update. J Cell Physiol 2019;234:19256-19270 https://doi.org/10.1002/jcp.28558
  97. Lockhart M, Wirrig E, Phelps A, Wessels A. Extracellular matrix and heart development. Birth Defects Res A Clin Mol Teratol 2011;91:535-550 https://doi.org/10.1002/bdra.20810
  98. Chen Q-Z, Harding SE, Ali NN, Lyon AR, Boccaccini AR. Biomaterials in cardiac tissue engineering: ten years of research survey. Mater Sci Eng R Rep 2008;59:1-37 https://doi.org/10.1016/j.mser.2007.08.001
  99. Hussain SH, Limthongkul B, Humphreys TR. The biomechanical properties of the skin. Dermatol Surg 2013;39:193-203 https://doi.org/10.1111/dsu.12095
  100. Norouzi M, Boroujeni SM, Omidvarkordshouli N, Soleimani M. Advances in skin regeneration: application of electrospun scaffolds. Adv Healthc Mater 2015;4:1114-1133 https://doi.org/10.1002/adhm.201500001
  101. Stevens MM. Biomaterials for bone tissue engineering. Mater Today 2008;11:18-25 https://doi.org/10.1016/S1369-7021(08)70086-5
  102. Ho DLL, Lee S, Du J, et al. Large-scale production of wholly cellular bioinks via the optimization of human induced pluripotent stem cell aggregate culture in automated bioreactors. Adv Healthc Mater 2022;11:e2201138
  103. Skylar-Scott MA, Uzel SGM, Nam LL, et al. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels. Sci Adv 2019;5:eaaw2459