International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Unleashing the Power of Undifferentiated Induced Pluripotent Stem Cell Bioprinting: Current Progress and Future Prospects

  • Boyoung Kim (Department of Biopharmaceutical Convergence, Sungkyunkwan University) ;
  • Jiyoon Kim (Department of Biopharmaceutical Convergence, Sungkyunkwan University) ;
  • Soah Lee (Department of Biopharmaceutical Convergence, Sungkyunkwan University)
  • Received : 2023.09.03
  • Accepted : 2023.11.21
  • Published : 2024.02.28
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Abstract

Induced pluripotent stem cell (iPSC) technology has revolutionized various fields, including stem cell research, disease modeling, and regenerative medicine. The evolution of iPSC-based models has transitioned from conventional two-dimensional systems to more physiologically relevant three-dimensional (3D) models such as spheroids and organoids. Nonetheless, there still remain challenges including limitations in creating complex 3D tissue geometry and structures, the emergence of necrotic core in existing 3D models, and limited scalability and reproducibility. 3D bioprinting has emerged as a revolutionary technology that can facilitate the development of complex 3D tissues and organs with high scalability and reproducibility. This innovative approach has the potential to effectively bridge the gap between conventional iPSC models and complex 3D tissues in vivo. This review focuses on current trends and advancements in the bioprinting of iPSCs. Specifically, it covers the fundamental concepts and techniques of bioprinting and bioink design, reviews recent progress in iPSC bioprinting research with a specific focus on bioprinting undifferentiated iPSCs, and concludes by discussing existing limitations and future prospects.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE, 2022R1A6A1A03054419), Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT, and Ministry of Health and Welfare (22A0302L1-01, Republic of Korea). The SungKyunKwan University and the BK21 FOUR (Graduate School Innovation) funded by the MOE and NRF.

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