Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Anti-inflammatory effect of beluga lentil extract in RAW 264.7 macrophages

RAW 264.7 대식세포에서 벨루가 렌틸 추출물의 항염증 효과

  • Hyeon-Ji Song (Department of Food Science and Technology, Keimyung University) ;
  • Syng-Ook Lee (Department of Food Science and Technology, Keimyung University)
  • 송현지 (계명대학교 식품가공학과) ;
  • 이승욱 (계명대학교 식품가공학과)
  • Received : 2024.04.18
  • Accepted : 2024.05.13
  • Published : 2024.06.30
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Abstract

The anti-inflammatory effect of beluga lentil extract (BLE) and its underlying mechanisms were investigated in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Treatment with BLE significantly decreased nitric oxide (NO) production and protein and mRNA expressions of inducible NO synthase (iNOS) in LPS-treated RAW 264.7 cells. Down-regulation of this inflammatory gene expression was not associated with NF-κB/MAPK signaling pathways, and further mechanistic studies demonstrated that BLE decreased LPS-induced iNOS expression through upregulation of the nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) expression. These results suggest that beluga lentil represent a potential source of natural anti-inflammatory agents, and further studies will be necessary to determine its anti-inflammatory effects in vivo.

본 연구에서는 벨루가 렌틸 추출물(BLE)의 항염증 효능을 NO 생성 측면에서 검토하고 이와 관련된 분자 기전을 규명하고자 하였다. LPS, LTA 및 지방세포 공동배양에 의해 활성화된 RAW 264.7 대식세포에서 BLE는 독성이 없는 농도 범위에서 유의적인 NO 생성 저해 효과를 보였다. 이와 함께 BLE 처리에 따라 iNOS mRNA 및 단백질의 발현이 유의적으로 저해됨을 확인하였으며, 이는 BLE에 의한 NO 생성 억제 효과가 전사 수준에서의 iNOS 발현을 억제함으로부터 기인하는 것임을 시사한다. 추가적으로 BLE는 NO 외에도 활성화된 대식세포에서 증가하는 다양한 염증성 사이토카인 유전자(COX-2, IL-1β 및 IL-6)의 mRNA 발현 또한 유의적으로 억제하는 것으로 나타났다. BLE의 항염증 효과와 관련된 분자 기전에 관한 추가적인 연구를 수행한 결과, 항염증 관련 대표 기전인 NF-κB/MAP kinases 신호 경로와는 연관성이 없는 것으로 나타났다. 반면, BLE 처리에 따라 Nrf2 단백질 발현의 증가 및 활성화가 유도되고 HO-1의 발현이 유의적으로 증가하는 것을 확인함으로써 Nrf2가 HO-1의 발현을 증가시키기 위한 전사 인자로서 작용할 가능성이 높음을 확인하였다. 따라서, 본 연구를 통해 식용원료 유래의 잠재적인 항염증 소재로서 BLE의 활용 가능성을 확인할 수 있었으며, 향후 동물모델을 대상으로 항염증 효과를 입증하기 위한 추가적인 연구가 필요할 것으로 생각된다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea [NRF-2023R1A2C1003582].

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