Journal of Microbiology and Biotechnology

  • 제34권4호
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  • Pages.949-957
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Melanin Inhibitory Effect of Tuber himalayense Isolated in Incheon, Korea

  • Byeong Min Choi (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University) ;
  • Minkyeong Kim (Biodiversity Research Department Species Diversity Research Division, National Institute of Biological Resources) ;
  • Hyehyun Hong (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University) ;
  • Tae-Jin Park (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University) ;
  • Changmu Kim (Biodiversity Research Department Species Diversity Research Division, National Institute of Biological Resources) ;
  • Jin-Soo Park (Natural Product Informatics Research Center, Korea Institute of Science and Technology) ;
  • Won-Jae Chi (Biodiversity Research Department Species Diversity Research Division, National Institute of Biological Resources) ;
  • Seung-Young Kim (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University)
  • 투고 : 2023.11.15
  • 심사 : 2024.01.18
  • 발행 : 2024.04.28
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초록

There has been a growing interest in skin beauty and antimelanogenic products. Melanogenesis is the process of melanin synthesis whereby melanocytes are activated by UV light or hormone stimulation to produce melanin. Melanogenesis is mediated by several enzymes, such as tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), and TRP-2. In this study, we investigated the effect of Tuber himalayense extract on melanin synthesis in α-melanocyte-stimulating hormone (α-MSH)-treated B16F10 melanoma cells. We confirmed that T. himalayense extract was not toxic to α-MSH-treated B16F10 melanoma cells and exhibited a significant inhibitory effect on melanin synthesis at concentrations of 25, 50, and 100 ㎍/ml. Additionally, the T. himalayense extract inhibited melanin, TRP-1, TRP-2, tyrosinase, and MITF, which are enzymes involved in melanin synthesis, in a concentration-dependent manner. Furthermore, T. himalayense extract inhibited the mitogen-activated protein kinase (MAPK) pathways, such as extracellular signal-regulated kinase-1/2 (ERK), c-Jun N-terminal kinase (JNK), and p38. Therefore, we hypothesized that various components of T. himalayense extract affect multiple factors involved in melanogenesis in B16F10 cells. Our results indicate that T. himalayense extract could potentially be used as a new material for preparing whitening cosmetics.

키워드

과제정보

This research was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202203112).

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