Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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1-Kestose Blocks UVB-Induced Skin Inflammation and Promotes Type I Procollagen Synthesis via Regulating MAPK/AP-1, NF-κB and TGF-β/Smad Pathway

  • Jihye Baek (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Jong-Hwa Kim (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Jiwon Park (Food R&D Center, Samyang Corp.) ;
  • Do Hyun Kim (Food R&D Center, Samyang Corp.) ;
  • Soonok Sa (Food R&D Center, Samyang Corp.) ;
  • Jung-Sook Han (Food R&D Center, Samyang Corp.) ;
  • Wonyong Kim (Department of Microbiology, Chung-Ang University College of Medicine)
  • Received : 2023.11.15
  • Accepted : 2024.02.17
  • Published : 2024.04.28
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Abstract

Solar UVB irradiation cause skin photoaging by inducing the high expression of matrix metalloproteinase (MMPs) to inhibit the expression of Type1 procollagen synthesis. 1-Kestose, a natural trisaccharide, has been indicated to show a cytoprotective role in UVB radiation-induced-HaCaT cells. However, few studies have confirmed the anti-aging effects. In the present study, we evaluated the anti-photoaging and pathological mechanism of 1-kestose using Human keratinocytes (HaCaT) cells. The results found that 1-kestose pretreatment remarkably reduced UVB-generated reactive oxygen species (ROS) accumulation in HaCaT cells. 1-Kestose suppressed UVB radiation-induced MMPs expressions by blocking MAPK/AP-1 and NF-κB p65 translocation. 1-Kestose pretreatment increased Type 1 procollagen gene expression levels by activating TGF-β/Smad signaling pathway. Taken together, our results demonstrate that 1-kestose may serve as a potent natural trisaccharide for inflammation and photoaging prevention.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), through the High Value-Added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (322025-03).

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