DOI QR코드

DOI QR Code

1-Kestose Blocks UVB-Induced Skin Inflammation and Promotes Type I Procollagen Synthesis via Regulating MAPK/AP-1, NF-κB and TGF-β/Smad Pathway

  • Jihye Baek (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Jong-Hwa Kim (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Jiwon Park (Food R&D Center, Samyang Corp.) ;
  • Do Hyun Kim (Food R&D Center, Samyang Corp.) ;
  • Soonok Sa (Food R&D Center, Samyang Corp.) ;
  • Jung-Sook Han (Food R&D Center, Samyang Corp.) ;
  • Wonyong Kim (Department of Microbiology, Chung-Ang University College of Medicine)
  • 투고 : 2023.11.15
  • 심사 : 2024.02.17
  • 발행 : 2024.04.28

초록

Solar UVB irradiation cause skin photoaging by inducing the high expression of matrix metalloproteinase (MMPs) to inhibit the expression of Type1 procollagen synthesis. 1-Kestose, a natural trisaccharide, has been indicated to show a cytoprotective role in UVB radiation-induced-HaCaT cells. However, few studies have confirmed the anti-aging effects. In the present study, we evaluated the anti-photoaging and pathological mechanism of 1-kestose using Human keratinocytes (HaCaT) cells. The results found that 1-kestose pretreatment remarkably reduced UVB-generated reactive oxygen species (ROS) accumulation in HaCaT cells. 1-Kestose suppressed UVB radiation-induced MMPs expressions by blocking MAPK/AP-1 and NF-κB p65 translocation. 1-Kestose pretreatment increased Type 1 procollagen gene expression levels by activating TGF-β/Smad signaling pathway. Taken together, our results demonstrate that 1-kestose may serve as a potent natural trisaccharide for inflammation and photoaging prevention.

키워드

과제정보

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), through the High Value-Added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (322025-03).

참고문헌

  1. Guo J, Huang X, Dou L, Yan M, Shen T, Tang W, et al. 2022. Aging and aging-related diseases: from molecular mechanisms to interventions and treatments. Signal Transduct. Target Ther. 7: 391.
  2. Jenkins G. 2002. Molecular mechanisms of skin ageing. Mech. Ageing Dev. 123: 801-810. https://doi.org/10.1016/S0047-6374(01)00425-0
  3. Wenk J, Brenneisen P, Meewes C, Wlaschek M, Peters T, Blaudschun R, et al. 2001. UV-induced oxidative stress and photoaging. Curr. Probl. Dermatol. 29: 83-94. https://doi.org/10.1159/000060656
  4. Anggakusuma, Yanti, Hwang JK. 2010. Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells. J. Dermatol. Sci. 57: 114-122. https://doi.org/10.1016/j.jdermsci.2009.10.005
  5. Papa S, Choy PM, Bubici C. 2019. The ERK and JNK pathways in the regulation of metabolic reprogramming. Oncogene 38: 2223-2240. https://doi.org/10.1038/s41388-018-0582-8
  6. Subedi L, Lee TH, Wahedi HM, Baek SH, Kim SY. 2017. Resveratrol-enriched rice attenuates UVB-ROS-induced skin aging via downregulation of inflammatory cascades. Oxid. Med. Cell. Longev. 2017: 8379539.
  7. Lee YR, Noh EM, Jeong EY, Yun SK, Jeong YJ, Kim JH, et al. 2009. Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappaB pathway in human dermal fibroblasts. Exp. Mol. Med. 41: 548-554. https://doi.org/10.3858/emm.2009.41.8.060
  8. Gao W, Wang YS, Hwang E, Lin P, Bae J, Seo SA, et al. 2018. Rubus idaeus L. (red raspberry) blocks UVB-induced MMP production and promotes type I procollagen synthesis via inhibition of MAPK/AP-1, NF-kappabeta and stimulation of TGF-beta/Smad, Nrf2 in normal human dermal fibroblasts. J. Photochem. Photobiol. B. 185: 241-253. https://doi.org/10.1016/j.jphotobiol.2018.06.007
  9. Nisar MF, Liu T, Wang M, Chen S, Chang L, Karisma VW, et al. 2022. Eriodictyol protects skin cells from UVA irradiation-induced photodamage by inhibition of the MAPK signaling pathway. J. Photochem. Photobiol. B. 226: 112350.
  10. Chen B, Li R, Yan N, Chen G, Qian W, Jiang HL, et al. 2015. Astragaloside IV controls collagen reduction in photoaging skin by improving transforming growth factor-beta/Smad signaling suppression and inhibiting matrix metalloproteinase-1. Mol. Med. Rep. 11: 3344-3348. https://doi.org/10.3892/mmr.2015.3212
  11. Lin PS, Chang HH, Yeh CY, Chang MC, Chan CP, Kuo HY, et al. 2017. Transforming growth factor beta 1 increases collagen content, and stimulates procollagen I and tissue inhibitor of metalloproteinase-1 production of dental pulp cells: role of MEK/ERK and activin receptor-like kinase-5/Smad signaling. J. Formos. Med. Assoc. 116: 351-358. https://doi.org/10.1016/j.jfma.2016.07.014
  12. Verrecchia F, Mauviel A. 2002. Transforming growth factor-beta signaling through the Smad pathway: role in extracellular matrix gene expression and regulation. J. Invest. Dermatol. 118: 211-215. https://doi.org/10.1046/j.1523-1747.2002.01641.x
  13. Shinohara M, Kiyosue M, Tochio T, Kimura S, Koga Y. 2020. Activation of butyrate-producing bacteria as well as bifidobacteria in the cat intestinal microbiota by the administration of 1-kestose, the smallest component of fructo-oligosaccharide. J. Vet. Med. Sci. 82: 866-874. https://doi.org/10.1292/jvms.19-0640
  14. Jovanovic-Malinovska R, Kuzmanova S, Winkelhausen E. 2015. Application of ultrasound for enhanced extraction of prebiotic oligosaccharides from selected fruits and vegetables. Ultrason. Sonochem. 22: 446-453. https://doi.org/10.1016/j.ultsonch.2014.07.016
  15. Tochio T, Kadota Y, Tanaka T, Koga Y. 2018. 1-Kestose, the smallest fructooligosaccharide component, which efficiently stimulates Faecalibacterium prausnitzii as well as bifidobacteria in humans. Foods 7: 140.
  16. Kim J-H, Baek J, Sa S, Park J, Kih M, Kim W. 2021. Kestose-enriched fructo-oligosaccharide alleviates atopic dermatitis by modulating the gut microbiome and immune response. J. Funct. Foods 85: 104650.
  17. Watanabe A, Tochio T, Kadota Y, Takahashi M, Kitaura Y, Ishikawa H, et al. 2021. Supplementation of 1-kestose modulates the gut microbiota composition to ameliorate glucose metabolism in obesity-prone hosts. Nutrients 13: 2983.
  18. Choi WJ, Konkit M, Kim Y, Kim MK, Kim W. 2016. Oral administration of Lactococcus chungangensis inhibits 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in NC/Nga mice. J. Dairy Sci. 99: 6889-6901. https://doi.org/10.3168/jds.2016-11301
  19. Yeo H, Lee JY, Kim J, Ahn SS, Jeong JY, Choi JH, et al. 2020. Transcription factor EGR-1 transactivates the MMP1 gene promoter in response to TNFalpha in HaCaT keratinocytes. BMB Rep. 53: 323-328. https://doi.org/10.5483/BMBRep.2020.53.6.290
  20. Kwon KR, Alam MB, Park JH, Kim TH, Lee SH. 2019. Attenuation of UVB-induced photo-aging by polyphenolic-rich spatholobus suberectus stem extract via modulation of MAPK/AP-1/MMPs signaling in human keratinocytes. Nutrients 11: 1341.
  21. Rabe JH, Mamelak AJ, McElgunn PJ, Morison WL, Sauder DN. 2006. Photoaging: mechanisms and repair. J. Am. Acad .Dermatol. 55: 1-19. https://doi.org/10.1016/j.jaad.2005.05.010
  22. Wang L, Oh JY, Lee W, Jeon YJ. 2021. Fucoidan isolated from Hizikia fusiforme suppresses ultraviolet B-induced photodamage by down-regulating the expressions of matrix metalloproteinases and pro-inflammatory cytokines via inhibiting NF-kappaB, AP-1, and MAPK signaling pathways. Int. J. Biol. Macromol. 166: 751-759. https://doi.org/10.1016/j.ijbiomac.2020.10.232
  23. Tochio T, Ueno Y, Kitaura Y, Shinohara M, Kadota Y, Minoda K, et al. 2019. Feeding of 1-kestose induces glutathione-S-transferase expression in mouse liver. Foods 8: 69.
  24. Guio F, Rodriguez MA, Almeciga-Diaz CJ, Sanchez OF. 2009. Recent trends in fructooligosaccharides production. Recent Pat. Food Nutr. Agric. 1: 221-230. https://doi.org/10.2174/2212798410901030221
  25. Liu S, You L, Zhao Y, Chang X. 2018. Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice. J. Agric. Food Chem. 66: 8537-8546. https://doi.org/10.1021/acs.jafc.8b02785
  26. He YL, Xiao Z, Yang S, Zhou C, Sun S, Hong P, et al. 2022. A Phlorotanin, 6,6'-bieckol from Ecklonia cava, against photoaging by inhibiting MMP-1, -3 and -9 expression on UVB-induced HaCaT keratinocytes. Photochem. Photobiol. 98: 1131-1139. https://doi.org/10.1111/php.13575
  27. Xiao Z, Yang S, Chen J, Li C, Zhou C, Hong P, et al. 2020. Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells. J. Funct. Foods 74: 104198.
  28. Kajanne R, Miettinen P, Mehlem A, Leivonen SK, Birrer M, Foschi M, et al. 2007. EGF-R regulates MMP function in fibroblasts through MAPK and AP-1 pathways. J. Cell. Physiol. 212: 489-497. https://doi.org/10.1002/jcp.21041
  29. Xiao Z, Liang P, Chen J, Chen MF, Gong F, Li C, et al. 2019. A Peptide YGDEY from Tilapia gelatin hydrolysates inhibits UVB-mediated skin photoaging by regulating MMP-1 and MMP-9 expression in HaCaT cells. Photochem. Photobiol. 95: 1424-1432. https://doi.org/10.1111/php.13135
  30. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. 1997. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl. J. Med. 337: 1419-1428. https://doi.org/10.1056/NEJM199711133372003
  31. Panichakul T, Ponnikorn S, Tupchiangmai W, Haritakun W, Srisanga K. 2022. Skin anti-aging potential of Ipomoea pes-caprae ethanolic extracts on promoting cell proliferation and collagen production in human fibroblasts (CCD-986sk Cells). Pharmaceuticals (Basel) 15: 969.