DOI QR코드

DOI QR Code

Lactobacillus Persisters Formation and Resuscitation

  • Hyein Kim (Department of Animal Science, Jeonbuk National University) ;
  • Sejong Oh (Division of Animal Science, Chonnam National University) ;
  • Sooyeon Song (Department of Animal Science, Jeonbuk National University)
  • 투고 : 2023.12.27
  • 심사 : 2024.01.16
  • 발행 : 2024.04.28

초록

Lactobacillus is a commonly used probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not Lactobacillus. Thus, understanding Lactobacillus persister cells will provide essential clues for understanding how Lactobacillus survives and maintains its function under various environmental conditions. We treated Lactobacillus strains with various antibiotics to determine the conditions required for persister formation using kill curves and transmission electron microscopy. In addition, we observed the resuscitation patterns of persister cells using single-cell analysis. Our results show that Lactobacillus creates a small population of persister cells (0.0001-1% of the bacterial population) in response to beta-lactam antibiotics such as ampicillin and amoxicillin. Moreover, only around 0.5-1% of persister cells are heterogeneously resuscitated by adding fresh media; the characteristics are typical of persister cells. This study provides a method for forming and verifying the persistence of Lactobacillus and demonstrates that antibiotic-induced Lactobacillus persister cells show characteristics of dormancy, sensitivity of antibiotics, same as exponential cells, multi-drug tolerance, and resuscitation, which are characteristics of general persister cells. This study suggests that the mechanisms of formation and resuscitation may vary depending on the characteristics, such as the membrane structure of the bacterial species.

키워드

과제정보

This work was supported by funds derived from a National Research Foundation of Korea (NRF) grant from the Korean Government (2020R1F1A107239713, RS-2023-00210305) for SYS and (NRF-2021R1A4A1031220) for SO. This paper was supported by research funds for newly appointed professors of Jeonbuk National University in 2020.

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