Journal of Microbiology and Biotechnology

  • 제34권4호
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  • Pages.774-782
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway

  • Ruiqi Liu (Jiangsu Province Academy of Traditional Chinese Medicine) ;
  • Bin Zhang (Nanjing Lishui District Hospital of Traditional Chinese Medicine) ;
  • Shuting Zou (Jiangsu Province Academy of Traditional Chinese Medicine) ;
  • Li Cui (Jiangsu Province Academy of Traditional Chinese Medicine) ;
  • Lin, Lin (Gastroenterology, Shenzhen Hospital of Guangzhou University of Chinese Medicine) ;
  • Lingchang Li (Jiangsu Province Academy of Traditional Chinese Medicine)
  • 투고 : 2023.10.27
  • 심사 : 2023.12.29
  • 발행 : 2024.04.28
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초록

This study aimed to elucidate the anti-colon cancer mechanism of ginsenoside Rg1 in vitro and in vivo. Cell viability rate was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tetrazolium assay. The inhibitory effect of ginsenoside Rg1 against CT26 cell proliferation gradually increased with increasing concentration. The in vivo experiments also demonstrated an antitumor effect. The monodansylcadaverine (MDC), transmission electron microscopy (TEM), and expression of autophagy marker proteins confirmed that ginsenoside Rg1 induced autophagy in vitro. Ginsenoside Rg1 induced autophagy death of CT26 cells, but this effect could be diminished by autophagy inhibitor (3-methyladenine, 3-MA). Additionally, in a xenograft model, immunohistochemical analysis of tumor tissues showed that the LC3 and Beclin-1 proteins were highly expressed in the tumors from the ginsenoside Rg1-treated nude mice, confirming that ginsenoside Rg1 also induced autophagy in vivo. Furthermoer, both in vivo and in vitro, the protein expressions of p-Akt, p-mTOR, and p-p70S6K were inhibited by ginsenoside Rg1, which was verified by Akt inhibitors. These results indicated that the mechanism of ginsenoside Rg1 against colon cancer was associated with autophagy through inhibition of the Akt/mTOR/p70S6K signaling pathway.

키워드

과제정보

This work was supported by the General Project of the National Clinical Research Base of Traditional Chinese Medicine(JD2019SZXYB04), the third batch of famous doctor reserve talents of Jiangsu Hospital of Integrated Traditional Chinese and Western Medicine, Su Traditional Chinese and Western medicine 2020-11(00051082), and the Scientific Research Project of Jiangsu Provincial Health Commission (M2021047).

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