Journal of Microbiology and Biotechnology

  • 제34권3호
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  • Pages.644-653
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Innate Immune-Enhancing Effect of Pinus densiflora Pollen Extract via NF-κB Pathway Activation

  • Sehyeon Jang (School of Food Science and Biotechnology, Kyungpook National University) ;
  • San Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Se Jeong Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Jun Young Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Da Hye Gu (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Bo Ram So (COSMAX NBT, INC.) ;
  • Jung A Ryu (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Jeong Min Park (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Sung Ran Yoon (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Sung Keun Jung (School of Food Science and Biotechnology, Kyungpook National University)
  • 투고 : 2023.09.19
  • 심사 : 2023.11.21
  • 발행 : 2024.03.28
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초록

Considering the emergence of various infectious diseases, including the coronavirus disease 2019 (COVID-19), people's attention has shifted towards immune health. Consequently, immune-enhancing functional foods have been increasingly consumed. Hence, developing new immune-enhancing functional food products is needed. Pinus densiflora pollen can be collected from the male red pine tree, which is commonly found in Korea. P. densiflora pollen extract (PDE), obtained by water extraction, contained polyphenols (216.29 ± 0.22 mg GAE/100 g) and flavonoids (35.14 ± 0.04 mg CE/100 g). PDE significantly increased the production of nitric oxide (NO) and reactive oxygen species (ROS) but, did not exhibit cytotoxicity in RAW 264.7 cells. Western blot results indicated that PDE induced the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. PDE also significantly increased the mRNA and protein levels of cytokines and the phosphorylation of IKKα/β and p65, as well as the activation and degradation of IκBα. Additionally, western blot analysis of cytosolic and nuclear fractions and immunofluorescence assay confirmed that the translocation of p65 to the nucleus after PDE treatment. These results confirmed that PDE increases the production of cytokines, NO, and ROS by activating NF-κB. Therefore, PDE is a promising nutraceutical candidate for immune-enhancing functional foods.

키워드

과제정보

This work was carried out with the support of "Development of K-Immunity Agricultural Food Technology Using Gyeongbuk Ingredients (Project No. LP0048882022)" Gyeongsangbuk-do Agricultural Research & Extension services and National Research Foundation of Korea Grant, funded by the Korean government (MEST) (NRF-2022R1A2C1010923).

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