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Inhibitory Effects of Latilactobacillus curvatus BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic Mice

  • Dingyun Li (Division of Animal Science, Chonnam National University) ;
  • Xing Wang (Division of Animal Science, Chonnam National University) ;
  • Dong-June Park (Korea Food Research Institute) ;
  • Dong Hun Lee (Department of Biological Sciences, Chonnam National University) ;
  • Sejong Oh (Division of Animal Science, Chonnam National University)
  • Received : 2023.09.04
  • Accepted : 2023.10.19
  • Published : 2024.03.28

Abstract

Latilactobacillus curvatus BYB3 (BYB3) is a species of lactic acid bacteria, formerly named Lactobacillus curvatus, which is isolated from kimchi. In this study, the effect of BYB3, Lactobacillus rhamnosus GG, and Lactobacillus acidophilus GP1B strain extracts at various concentrations was examined on B16F10, a mouse melanoma cell line. Cell viability was examined via MTT assay, and the results indicated that compared to the other two probiotics, BYB3 significantly decreased the total percentages of viable cells. The effects of BYB3 on cell migration and proliferation in B16F10 cells were evaluated using wound healing mobility and proliferation assays, respectively; the results indicated that BYB3 inhibits cell migration and proliferation in a concentration-dependent manner. Using human dermal fibroblast cells to investigate BYB3 extract in vivo had no effect on skin-related cells. Nonetheless, the BYB3 extract inhibited tumor growth in a mouse model, as demonstrated by liver slices. Therefore, this suggests that using BYB3 extract to inhibit melanoma may be a novel approach.

Keywords

Acknowledgement

This work was supported by the National Research Foundation grant (NRF) grant funded by the Korea government (2021R1A4A1031220 and RS-2023-00209264).

References

  1. Davis LE, Shalin SC, Tackett AJ. 2019. Current state of melanoma diagnesis and treatment. Cancer Biol. Ther. 20: 1366-1379. https://doi.org/10.1080/15384047.2019.1640032
  2. Liu Q, Das M, Liu Y, Huang L. 2018. Targeted drug delivery to melanoma. Adv. Drug Deliv. Rev. 127: 208-221. https://doi.org/10.1016/j.addr.2017.09.016
  3. Schadendorf D, Fisher DE, Garbe C, Gershenwald JE, Grob JJ, Halpern A, et al. 2015. Melanoma. Nat. Rev. Dis. Primers 1: 15003.
  4. Nieto MA, Huang RYJ, Jackson RA, Thiery JP. 2016. EMT: 2016. Cell 166: 21-45. https://doi.org/10.1016/j.cell.2016.06.028
  5. Ye Q, Wang X, Yuan M, Cui S, Chen Y, Hu Z, et al. 2021. miR-219-5p targets TBXT and inhibits breast cancer cell EMT and cell migration and invasion. Biosci. Rep. 41: BSR20210318.
  6. Zielinska D, Kolozyn-Krajewska D. 2018. Food-origin lactic acid bacteria may exhibit probiotic properties. Biomed Res. Int. 2018: 5063185.
  7. Park YH, Kim JG, Shin YW, Kim SH, Whang KY. 2007. Effect of dietary inclusion of Lactobacillus acidophilus ATCC 43121 on cholesterol metabolism in rats. J. Microbiol. Biotechnol. 17: 655-662.
  8. Lewis-Mikhael A-M, Davoodvandi A, Jafarnejad S. 2020. Effect of Lactobacillus plantarum containing probiotics on blood pressure: a systematic review and meta-analysis. Pharmacol. Res. 153: 104663.
  9. Ding C, Tang W, Fan X, Wu G. 2018. Intestinal microbiota: a novel perspective in colorectal cancer biotherapeutics. OncoTargets Ther. 11: 4797-4810. https://doi.org/10.2147/OTT.S170626
  10. El-Deeb NM, Yassin AM, Al-Madboly LA, El-Hawiet A. 2018. A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer. Microb. Cell Fact. 17: 29.
  11. Wang X, Li D, Meng Z, Kim K, Oh S. 2022. Latilactobacillus curvatus BYB3 isolated from kimchi alleviates dextran sulfate sodium (DSS)-induced Colitis in mice by inhibiting IL-6 and TNF-R1 production. J. Microbiol. Biotechnol. 32: 348-354. https://doi.org/10.4014/jmb.2109.09054
  12. Nowak A, Zaklos-Szyda M, Rosicka-Kaczmarek J, Motyl I. 2022. Anticancer potential of post-fermentation media and cell extracts of probiotic strains: an in vitro study. Cancers 14: 1853.
  13. Chen Y, Huang B, He J, Han L, Zhan Y, Wang Y. 2011. In vitro and in vivo antioxidant effects of the ethanolic extract of Swertia chirayita. J. Ethnopharmacol. 136: 309-315. https://doi.org/10.1016/j.jep.2011.04.058
  14. Rupasinghe H, Parmar I, Neir SV. 2019. Biotransformation of cranberry proanthocyanidins to probiotic metabolites by Lactobacillus rhamnosus enhances their anticancer activity in HepG2 cells in vitro. Oxid. Med. Cell. Longev. 2019: 4750795.
  15. Ma EL, Choi YJ, Choi J, Pothoulakis C, Rhee SH, Im E. 2010. The anticancer effect of probiotic Bacillus polyfermenticus on human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition. Int. J. Cancer 127: 780-790. https://doi.org/10.1002/ijc.25011
  16. Comamala M, Pinard M, Theriault C, Matte I, Albert A, Boivin M, et al. 2011. Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH: OVCAR3 ovarian carcinoma cells. Br. J. Cancer 104: 989-999. https://doi.org/10.1038/bjc.2011.34
  17. Yue Y, Ye K, Lu J, Wang X, Zhang S, Liu L, et al. 2020. Probiotic strain Lactobacillus plantarum YYC-3 prevents colon cancer in mice by regulating the tumour microenvironment. Biomed. Pharmacother. 127: 110159.
  18. Motevaseli E, Dianatpour A, Ghafouri-Fard S. 2017. The role of probiotics in cancer treatment: emphasis on their in vivo and in vitro anti-metastatic effects. Int. J. Mol. Cell. Med. 6: 66-76.