Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Characteristics of Extended-Spectrum Beta-Lactamase-Producing Salmonella Isolated from Retail Meats in South Korea

  • Haiseong Kang (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Hansol Kim (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Hyochin Kim (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Ji Hye Jeon (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Seokhwan Kim (Food Standard Division, Ministry of Food and Drug Safety) ;
  • Yongchjun Park (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Soon Han Kim (Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation)
  • Received : 2023.12.12
  • Accepted : 2024.03.03
  • Published : 2024.05.28
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Abstract

Earlier studies have validated the isolation of extended-spectrum beta-lactamase-producing Salmonella (ESBL-Sal) strains from food. While poultry is recognized as a reservoir for Salmonella contamination, pertinent data regarding ESBL-Sal remains limited. Consequently, the Ministry of Food and Drug Safety has isolated Salmonella spp. from retail meat and evaluated their antibiotic susceptibility and genetic characteristics via whole-genome sequencing. To further elucidate these aspects, this study investigates the prevalence, antibiotic resistance profiles, genomic characteristics, and homology of ESBL-Sal spp. obtained from livestock-derived products in South Korean retail outlets. A total of 653 Salmonella spp. were isolated from 1,876 meat samples, including 509 beef, 503 pork, 555 chicken, and 309 duck samples. The prevalence rates of Salmonella were 0.0%, 1.4%, 17.5%, and 28.2% in the beef, pork, chicken, and duck samples, respectively. ESBL-Sal was exclusively identified in poultry meat, with a prevalence of 1.4% in the chicken samples (8/555) and 0.3% in the duck samples (1/309). All ESBL-Sal strains carried the blaCTX-M-1 gene and exhibited resistance to ampicillin, ceftiofur, ceftazidime, nalidixic acid, and tetracycline. Eight ESBL-Sal isolates were identified as S. Enteritidis with sequence type (ST) 11. The major plasmid replicons of the Enteritidis-ST11 strains were IncFIB(S) and IncFII(S), carrying antimicrobial resistance genes (β-lactam, tetracycline, and aminoglycoside) and 166 virulence factor genes. The results of this study provide valuable insights for the surveillance and monitoring of ESBL-Sal in South Korean food chain.

Keywords

Acknowledgement

This research was supported by grants (Nos. 15161MFDS645 and 20161MFDS009) from the Ministry of Food and Drug Safety of Korea. The results and conclusions of the study are the sole property of the authors and do not necessarily represent the views of the Ministry of Food and Drug Safety. We thank the laboratories and center members for their contribution to the collection of meat samples for the isolation of Salmonella strains used in this study. We would like to thank Editage (www.editage.co.kr) for English language editing.

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