Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibition of Verticillium Wilt in Cotton through the Application of Pseudomonas aeruginosa ZL6 Derived from Fermentation Residue of Kitchen Waste

  • Qiuhong Niu (College of Life Science and Agricultural Engineering, Nanyang Normal University) ;
  • Shengwei Lei (College of Life Science and Agricultural Engineering, Nanyang Normal University) ;
  • Guo Zhang (College of Agriculture and Engineering, Nanyang Vocational College of Agriculture) ;
  • Guohan Wu (College of Life Science and Agricultural Engineering, Nanyang Normal University) ;
  • Zhuo Tian (College of Life Science and Agricultural Engineering, Nanyang Normal University) ;
  • Keyan Chen (College of Life Science and Agricultural Engineering, Nanyang Normal University) ;
  • Lin Zhang (College of Life Science and Agricultural Engineering, Nanyang Normal University)
  • Received : 2024.01.29
  • Accepted : 2024.03.25
  • Published : 2024.05.28
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Abstract

To isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas aeruginosa. Bioassay results demonstrated that this strain possessed robust antagonistic abilities, effectively inhibiting V. dahliae spore germination and mycelial growth. Furthermore, P. aeruginosa ZL6 exhibited high temperature resistance (42℃), nitrogen fixation, and phosphorus removal activities. Pot experiments revealed that P. aeruginosa ZL6 fermentation broth treatment achieved a 47.72% biological control effect compared to the control group. Through activity tracking and protein mass spectrometry identification, a neutral metalloproteinase (Nml) was hypothesized as the main virulence factor. The mutant strain ZL6ߡNml exhibited a significant reduction in its ability to inhibit cotton Verticillium wilt compared to the strain P. aeruginosa ZL6. While the inhibitory activities could be partially restored by a complementation of nml gene in the mutant strain ZL6CMߡNml. This research provides a theoretical foundation for the future development and application of biogas residue as biocontrol agents against Verticillium wilt and as biological preservatives for agricultural products. Additionally, this study presents a novel approach for mitigating the substantial amount of biogas residue generated from kitchen waste fermentation.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation Program of China (3217010010), the Program for Outstanding Youth Science Fund Project of Henan Province (222300420014), Agricultural Biomass Green Conversion Technology University Scientific Innovation Team in Henan Province (24IRTSTHN036), by Special Fund for Doctor in Nanyang Normal University (2022ZX031), and by open subject from National Key Laboratory of cotton biological breeding and comprehensive utilization (CB2023A11).

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