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Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice

  • Khammool Khamsuk (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University) ;
  • Bernard Dell (Centre for Crop and Food Innovation, Murdoch University) ;
  • Wasu Pathom-aree (Department of Biology, Faculty of Science, Chiang Mai University) ;
  • Wanwarang Pathaichindachote (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University) ;
  • Nungruthai Suphrom (Center of Excellence in Biomaterials, Naresuan University) ;
  • Nareeluk Nakaew (Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University) ;
  • Juangjun Jumpathong (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University)
  • Received : 2024.02.05
  • Accepted : 2024.03.25
  • Published : 2024.05.28

Abstract

This study explores beneficial bacteria isolated from the roots and rhizosphere soil of Khao Rai Leum Pua Phetchabun rice plants. A total of 315 bacterial isolates (KK001 to KK315) were obtained. Plant growth-promoting traits (phosphate solubilization and indole-3-acetic acid (IAA) production), and antimicrobial activity against three rice pathogens (Curvularia lunata NUF001, Bipolaris oryzae 2464, and Xanthomonas oryzae pv. oryzae) were assessed. KK074 was the most prolific in IAA production, generating 362.6 ± 28.0 ㎍/ml, and KK007 excelled in tricalcium phosphate solubilization, achieving 714.2 ± 12.1 ㎍/ml. In antimicrobial assays using the dual culture method, KK024 and KK281 exhibited strong inhibitory activity against C. lunata, and KK269 was particularly effective against B. oryzae. In the evaluation of antimicrobial metabolite production, KK281 and KK288 exhibited strong antifungal activities in cell-free supernatants. Given the superior performance of KK281, taxonomically identified as Bacillus sp. KK281, it was investigated further. Lipopeptide extracts from KK281 had significant antimicrobial activity against C. lunata and a minimum inhibitory concentration (MIC) of 3.1 mg/ml against X. oryzae pv. oryzae. LC-ESI-MS/MS analysis revealed the presence of surfactin in the lipopeptide extract. The crude extract was non-cytotoxic to the L-929 cell line at tested concentrations. In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.

Keywords

Acknowledgement

This work was financially supported by Research and Researcher for industry (RRi), Thailand Science Research and Innovation (TSRI) and Banrai-ioon strawberry farm (Grant No. MSD62I0098). Special thanks are extended to Dr. Sakamon Devahastin for his constructive comments and to Dr. Kumrop Ratanasut for providing the phytopathogenic bacterium.

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