Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Fermented Milk Containing Lacticaseibacillus rhamnosus SNU50430 Modulates Immune Responses and Gut Microbiota in Antibiotic-Treated Mice

  • Sunghyun Yoon (Graduate School of Public Health, Seoul National University) ;
  • SungJun Park (N-Bio, Seoul National University) ;
  • Seong Eun Jung (R&BD Center, hy Co., Ltd.) ;
  • Cheonghoon Lee (Graduate School of Public Health, Seoul National University) ;
  • Woon-Ki Kim (Graduate School of Public Health, Seoul National University) ;
  • Il-Dong Choi (R&BD Center, hy Co., Ltd.) ;
  • GwangPyo Ko (Graduate School of Public Health, Seoul National University)
  • Received : 2024.01.15
  • Accepted : 2024.04.15
  • Published : 2024.06.28
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Abstract

Antibiotics are used to control infectious diseases. However, adverse effects of antibiotics, such as devastation of the gut microbiota and enhancement of the inflammatory response, have been reported. Health benefits of fermented milk are established and can be enhanced by the addition of probiotic strains. In this study, we evaluated effects of fermented milk containing Lacticaseibacillus rhamnosus (L. rhamnosus) SNUG50430 in a mouse model with antibiotic treatment. Fermented milk containing 2 × 105 colony-forming units of L. rhamnosus SNUG50430 was administered to six week-old female BALB/c mice for 1 week. Interleukin (IL)-10 levels in colon samples were significantly increased (P < 0.05) compared to water-treated mice, whereas interferon-gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) were decreased, of mice treated with fermented milk containing L. rhamnosus SNUG50430-antibiotics-treated (FM+LR+Abx-treated) mice. Phylum Firmicutes composition in the gut was restored and the relative abundances of several bacteria, including the genera Coprococcus and Lactobacillus, were increased in FM+LR+Abx-treated mice compared to PBS+Abx-treated mice. Interestingly, abundances of genus Coprococcus and Lactobacillus were positively correlated with IL-5 and IL-10 levels (P < 0.05) in colon samples and negative correlated with IFN-γ and TNF-α levels in serum samples (P < 0.001). Acetate and butyrate were increased in mice with fermented milk and fecal microbiota of FM+LR+Abx-treated mice were highly enriched with butyrate metabolism pathway compared to water-treated mice (P < 0.05). Thus, fermented milk containing L. rhamnosus SNUG50430 was shown to ameliorate adverse health effects caused by antibiotics through modulating immune responses and the gut microbiota.

Keywords

Acknowledgement

This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through a High Value-added Food Technology Development Program funded by Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (315067-3), the Bio & Medical Technology Development Program the National Research Foundation of Korea (NRF) funded by the Korean government (MSIT) (NRF-2022M3A9F3017371), and the Basic Science Research Program through the NRF funded by the Ministry of Education (NRF-2021R1I1A1A01048923).

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