Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide

  • Jae Min Hwang (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Mun-Hoe Lee (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Yuri Kwon (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Hee-Chul Chung (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Do-Un Kim (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Jin-Hee Lee (Health Food Research and Development, NEWTREE Co., Ltd.)
  • Received : 2023.07.14
  • Accepted : 2023.11.09
  • Published : 2024.02.28
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Abstract

This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally, mRNA levels of bone metabolism-related factors such as the collagen type I alpha 1 chain, osteocalcin (OCN), osterix, bone sialoprotein, and the Runt family-associated transcription factor 2 were increased in cells treated with 1,000 ㎍/ml of LMWCP. Furthermore, we demonstrated that critical bone morphometric parameters exhibited significant differences between the LMWCP (400 mg/kg)-receiving and vehicle-treated rat groups. Moreover, the expression of type I collagen and the activity of ALP were found to be higher in both the femur and lumbar vertebrae of OVX rats treated with LMWCP. Finally, the administration of LMWCP managed to alleviate osteogenic parameters such as the ALP activity and the levels of the bone alkaline phosphatase, the OCN, and the procollagen type 1 N-terminal propeptide in OVX rats. Thus, our findings suggest that LMWCP is a promising candidate for the development of food-based prevention strategies against osteoporosis.

Keywords

Acknowledgement

This work was supported by NEWTREE Co., Ltd., Korea.

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