Journal of Microbiology and Biotechnology

  • 제34권2호
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  • Pages.367-378
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Fenchone Ameliorates Constipation-Predominant Irritable Bowel Syndrome via Modulation of SCF/c-Kit Pathway and Gut Microbiota

  • Li Cui (Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine) ;
  • Bin Zhang (Digestive Department, Nanjing Lishui District Hospital of Traditional Chinese Medicine) ;
  • Shuting Zou (Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine) ;
  • Jing Liu (Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine) ;
  • Pingrong Wang (Jinling Clinical Medical College, Nanjing University of Chinese Medicine) ;
  • Hui Li (Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine) ;
  • Zhenhai Zhang (Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine)
  • 투고 : 2023.08.08
  • 심사 : 2023.10.16
  • 발행 : 2024.02.28
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초록

In this study we sought to elucidate the therapeutic effects of fenchone on constipation-predominant irritable bowel syndrome (IBS-C) and the underlying mechanisms. An IBS-C model was established in rats by administration of ice water by gavage for 14 days. Fenchone increased the reduced body weight, number of fecal pellets, fecal moisture, and intestinal transit rate, and decreased the enhanced visceral hypersensitivity in the rat model of IBS-C. In addition, fenchone increased the serum content of excitatory neurotransmitters and decreased the serum content of inhibitory neurotransmitters in the IBS-C rat model. Meanwhile, western blot and immunofluorescence experiments indicated that fenchone increased the expressions of SCF and c-Kit. Furthermore, compared with the IBS-C model group, fenchone increased the relative abundance of Lactobacillus, Blautia, Allobaculum, Subdoligranulum, and Ruminococcaceae_UCG-008, and reduced the relative abundance of Bacteroides, Enterococcus, Alistipes, and Escherichia-Shigella on the genus level. Overall, fenchone ameliorates IBS-C via modulation of the SCF/c-Kit pathway and gut microbiota, and could therefore serve as a novel drug candidate against IBS-C.

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과제정보

This work was financially supported by the National Natural Science Foundation of China (81403121), Jiangsu Provincial TCM Science and Technology Development Plan Project (MS2021025), Social Development Project of Jiangsu Province (BE2022817), and the central government financial transfer payment for local projects from the National Administration of Traditional Chinese Medicine (2022GJJZDYJS-01).

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