Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Listeria Species by Conventional Culture-Dependent and Alternative Rapid Detection Methods in Retail Ready-to-Eat Foods in Turkey

  • Emine Dincer (Faculty of Health Sciences, Department of Nutrition and Dietetics, Sivas Cumhuriyet University)
  • Received : 2023.08.24
  • Accepted : 2023.10.30
  • Published : 2024.02.28
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Abstract

Foodborne pathogens, like Listeria monocytogenes, continue to inflict substantial financial losses on the food industry. Various methods for detecting Listeria in food have been developed and numerous studies have been conducted to compare the different methods. But, in recent years, new Listeria species have been identified, and currently the genus comprises 26 species. Therefore, it would be a more accurate approach to re-evaluate existing detection methods by considering new species. The present investigation involved the analysis of 42 ready-to-eat (RTE) foods, encompassing a variety of food categories, such as mezes, salads, dairy products, and meat products, with the aim of ascertaining the presence of Listeria. Among the traditional culture-dependent reference methods, the ISO 11290 method was preferred. The process of strain identification was conducted with the API Identification System. Furthermore, to ascertain the existence of L. monocytogenes and Listeria spp., the samples underwent additional analysis employing the VIDAS Immunoassay System, ELISA, and RT-PCR methodologies. Thus, four alternative approaches were employed in this study to compare not only the different methods used to determine Listeria while taking into account the newly identified Listeria species, but also to assess the compliance of retail RTE food items with microbiological criteria pertaining to the genus Listeria. Based on the conducted analyses, L. monocytogenes was conclusively determined to be present in one sample. The presence of Listeria spp. was detected in 30.9% of the samples, specifically in Turkish cig kofte, sliced salami, and salads.

Keywords

Acknowledgement

E. Dincer especially thanks Prof. Dr. Merih Kivanc for making her laboratory available.

References

  1. Nwaiwu O. 2020 What are the recognized species of the genus Listeria? Access Microbiol. 2: acmi000153. 
  2. Carlin CR, Liao J, Hudson LK, Peters TL, Denes TG, Orsi RH, et al. 2022. Soil collected in the Great Smoky Mountains National Park yielded a novel Listeria sensu stricto species, L. swaminathanii. Microbiol. Spect. 10: e00442-22. 
  3. Carlin CR, Roof S, Wiedmann M. 2022. Assessment of reference method selective broth and plating media with 19 Listeria species highlights the importance of including diverse species in Listeria method evaluations. J. Food Prot. 8: 494-510. 
  4. Sanlibaba P, Tezel BU, Cakmak GA. 2018. Prevalence and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat foods in Turkey. J. Food Qual. 2018: 7693782. 
  5. Duze ST, Marimani M, Patel M. 2021. Tolerance of Listeria monocytogenes to biocides used in food processing environments. Food Microbiol. 97: 103758. 
  6. Zhang X, Wang S, Chen X, Qu C. 2021. Review controlling Listeria monocytogenes in ready-to-eat meat and poultry products: an overview of outbreaks, current legislations, challenges, and future prospects. Trends Food Sci. Technol. 116: 24-35. 
  7. EFSA and ECDC. 2021. The European Union one health 2019 zoonoses report. EFSA J. 19: 6406. 
  8. Iannetti L, Schirone M, Neri D, Visciano P, Acciari VA, Centorotola G, et al. 2020. Listeria monocytogenes in poultry: detection and strain characterization along an integrated production chain in Italy. Food Microbiol. 91: 103533. 
  9. Becker N, Schewe T, Setzer FK, Schroder M, Reckzeh C, Vossenkuhl B, et al. 2021. Identification of the source of a Listeria monocytogenes outbreak by investigational tracing. J. Verbrauch Lebensm. 16: 205-212. 
  10. Neri D, Antoci S, Iannetti L, Ciorba AB, D'Aurelio R, Del Matto I, et al. 2019. EU and US control measures on Listeria monocytogenes and Salmonella spp. in certain ready-to-eat meat products: an equivalence study. Food Control 96: 98-103. 
  11. Townsend A, Strawn LK, Chapman BJ, Dunn LL. 2021. A systematic review of Listeria species and Listeria monocytogenes prevalence, persistence, and diversity throughout the fresh produce supply chain. Foods 10: 1427. 
  12. Lepecka A, Zielinska D, Szymanski P, Buras I, Kolozyn-Krajewska D. 2022. Assessment of the microbiological quality of ready-to-eat salads-are there any reasons for concern about public health?. Int. J. Environ. Res. Public Health 19: 1582. 
  13. Valimaa AL, Tilsala-Timisjarvi A, Virtanen E. 2015. Rapid detection and identification methods for Listeria monocytogenes in the food chain-a review. Food Control 55: 103-114. 
  14. Liu A, Shen L, Zen, Z, Sun M, Liu Y, Liu S, et al. 2018. A mini review of the methods for Listeria monocytogenes detection. Food Anal. Methods 11: 215-223. 
  15. Gupta P, Adhikari A. 2022. Novel approaches to environmental monitoring and control of Listeria monocytogenes in food production facilities. Foods 11: 1760. 
  16. Osek J, Lachtara B, Wieczorek K. 2022. Listeria monocytogenes in foods-From culture identification to whole-genome characteristics. Food Sci. Nutr. 10: 2825-2854. 
  17. EN ISO 11290-1:2017. 2017. Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method. Geneva, Switzerland: International Organization for Standardization (ISO). 
  18. Aznar R, Solis I. 2006. PCR detection of Listeria monocytogenes in different food products compared with the mini-VIDAS LMO system and the standard procedure ISO 11290-1. J. Verbrauch Lebensm. 1: 115-120. 
  19. Barre L, Angelidis AS, Boussaid D, Brasseur ED, Manso E, Besse NG. 2016. Applicability of the EN ISO 11290-1 standard method for Listeria monocytogenes detection in presence of new Listeria species. Int. J. Food Microbiol. 238: 281-287. 
  20. Gasanov U, Hughes D, Hansbro PM. 2005. Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiol Rev. 29: 851-875. 
  21. Noah CW, Ramos NC, Gipson VM. 1991. Efficiency of two commercial ELISA kits compared with the BAM culture method for detecting Listeria in naturally contaminated foods. J. Assoc. Off. Anal. Chem. 74: 819-821. 
  22. Vanderlinde PB, Grau FH. 1991. Detection of Listeria spp. in meat and environmental samples by an Enzyme-Linked Immunosorbent Assay (ELISA). J. Food Prot. 54: 230-231. 
  23. Netschajew A, Fredriksson-Ahomaa M, Sperner B, Stolle A. 2009. Detection of Listeria monocytogenes in vacuum-packed meat products using real-time PCR, immunoassay and culturing. Arch. Lebensmittelhyg. 60: 12-17. 
  24. Barbau-Piednoir E, Botteldoorn N, Yde M, Mahillon J, Roosens, NH. 2013. Development and validation of qualitative SYBR Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes. Appl. Microbiol. Biotechnol. 97: 4021-4037. 
  25. McLauchlin J. 1997. The identification of Listeria species. Int. J. Food Microbiol. 38: 77-81. 
  26. Bernardi G, Abrahao WM, Benetti TM, de Souza VR, de Francisco TG. 2015. Evaluation of the detection methods used for investigation of Listeria and Listeria monocytogenes. J. Food Nutr. Disor. 4: 6. 
  27. Micropest. Summary report according to the standard EN ISO 16140-2:2016. VIDAS Listeria monocytogenes II (VIDAS LMO2 - Ref. 30704) (certificate # BIO 12/11-03/04) for the detection of Listeria monocytogenes in human food products and in environmental samples. 2020. Available from: https://nf-validation.afnor.org/wp-content/uploads/2014/03/Synt-BIO-12-11-03-04_en.pdf. Accessed Nov. 5, 2022. 
  28. Reiter MG, Lopez C, Jordano R, Medina LM. 2010. Comparative study of alternative methods for food safety control in poultry slaughterhouses. Food Anal. Methods. 3: 253-260. 
  29. Estevez M, Garcia-Viejo F, Lopez-Mendoza MC, Jordano R, Medina LM. 2021. Influence of food matrices and the population of interfering microorganisms on the determination of Listeria monocytogenes by conventional methods and VIDAS. Foods 10: 3021. 
  30. Vaz-Velho M, Duarte G, Gibbs P. 2000. Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish. J. Microbiol. Methods 40: 147-151. 
  31. Bilgin Z, Bayrakal GM, Dumen E, Ekici G. 2019. Prevalence and PCR Sensitivity comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in salads and appetizers consumed in Istanbul. Turk J. Agric. Food Sci. Technol. 7: 737-742. 
  32. Bustamante F, Maury-Sintjago E, Leal FC, Acuna S, Aguirre J, Troncoso M, et al. 2020. Presence of Listeria monocytogenes in ready-to-eat artisanal Chilean foods. Microorganisms 8: 1669.