Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Aptamer Based SPREETA Sensor for the Detection of Porphyromonas gingivalis G-Protein

  • Suk-Gyun Park (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Hyun Ju Lee (Department of Cosmetic Science, Kwangju Women's University) ;
  • Taeksoo Ji (School of Electronics and Computer Engineering, Chonnam National University) ;
  • Kyungbaek Kim (School of Electronics and Computer Engineering, Chonnam National University) ;
  • Seung-Ho Ohk (Department of Oral Microbiology, School of Dentistry, Chonnam National University)
  • Received : 2023.10.26
  • Accepted : 2023.12.11
  • Published : 2024.02.28
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Abstract

We have developed an aptamer that specifically binds to Porphyromonas gingivalis to reduce the cellular damage caused by P. gingivalis infection and applied it as a biosensor. P. gingivalis is one of the major pathogens causing destructive periodontal disease among the periodontal microorganisms constituting complex biofilms. Porphyromonas gingivalis G-protein (PGP) known to play an important role in the transmission of germs was used as a target protein for the screening of aptamer. The aptamer that has binds to the G-protein of P. gingivalis, was screened and developed through the Systemic Evolution of Ligands by Exponential Energy (SELEX) method. Modified-Western blot analysis was performed with the aptamer which consisted of 38 single-stranded DNA to confirm the selectivity. ELONA (enzyme linked oligonucleotide assay) used to confirm that the aptamer was sensitive to PGP even at low concentration of 1 ㎍/ml. For the rapid detection of P. gingivalis, we constructed a surface plasmon resonance biosensor with SPREETA using the PGP aptamer. It was confirmed that PGP could be detected as low concentration as at 0.1 pM, which is the minimum concentration of aptamer sensor within 5 min. Based on these results, we have constructed a SPREETA biosensor based on aptamer that can bind to P. gingivalis G-protein. It can be used as an infection diagnosis system to rapidly diagnose and analyze oral diseases caused by P. gingivalis.

Keywords

Acknowledgement

This study was supported by a grant (CRI 15006-1) Chonnam National University Hospital Biomedical Research Institute.

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