Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-Inflammatory Activity of Vacuum Distillate from Panax ginseng Root on LPS-Induced RAW264.7 Cells

  • Chanwoo Lee (College of Pharmacy, Kyung Hee University) ;
  • Seul Lee (Department of Engineering Chemistry, Chungbuk National University) ;
  • Young Pyo Jang (College of Pharmacy, Kyung Hee University) ;
  • Junseong Park (Department of Engineering Chemistry, Chungbuk National University)
  • Received : 2023.12.04
  • Accepted : 2024.01.05
  • Published : 2024.02.28
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Abstract

Panax ginseng has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of P. ginseng distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on lipopolysaccharide-induced macrophages was assessed. The results showed that GSD effectively inhibited nitric oxide (NO) formation and activation of inducible nitric oxide synthase (iNOS) mRNA in murine macrophage cell, but not cyclooxygenase-2 production. The mRNA expression pattern of tumor necrosis factor alpha and IL-6 were also reduced by GSD. Furthermore, we confirmed that GSD exerted its anti-inflammatory effects by downregulating c-Jun NH2-terminal kinase (JNK) phosphorylation, the extracellular signal-regulated kinase phosphorylation, and signaling pathway of nuclear factor kappa B (NF-κB). Our findings revealed that the inflammatory regulation activity of GSD could be induced by iNOS and NO formation inhibition mediated by regulation of nuclear factor kappa B and p38/JNK MAPK pathways.

Keywords

Acknowledgement

This research was supported by the Leaders in Industry-University Cooperation 3.0 (LINC 3.0) Project (NIBR), funded by the Ministry of Education and National Research Foundation of the Republic of Korea (Grant No. 1345370624, and Regional Innovation Strategy (RIS) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (2021RIS-001).

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