Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effects of the Processing Methods of Forage Rye (Secale cereale) on Rumen Fermentation Characteristics and Greenhouse Gas Emissions In Vitro of Hanwoo

호밀 조사료 가공방법이 한우의 반추위 내 발효특성과 온실가스 발생량에 미치는 영향

  • Ji Yoon Kim (Division of Applied Life Science (BK21Four), Gyeongsang National University) ;
  • Seung Min Jeong (Grassland and Forages Division, National Institute of Animal Science, RDA) ;
  • Young Ho Joo (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Chang Hyun Baeg (Division of Applied Life Science (BK21Four), Gyeongsang National University) ;
  • Bu Gil Choi (Division of Applied Life Science (BK21Four), Gyeongsang National University) ;
  • Arrynda Rachma Dyasti Wardani (Division of Applied Life Science (BK21Four), Gyeongsang National University) ;
  • Sam Churl Kim (Division of Applied Life Science (BK21Four), Gyeongsang National University)
  • 김지윤 (경상국립대학교 응용생명과학부(BK21Four)) ;
  • 정승민 (국립축산과학원 초지사료과) ;
  • 주영호 (경상국립대학교 농업생명과학연구원) ;
  • 백창현 (경상국립대학교 응용생명과학부(BK21Four)) ;
  • 최부길 (경상국립대학교 응용생명과학부(BK21Four)) ;
  • 애린다 (경상국립대학교 응용생명과학부(BK21Four)) ;
  • 김삼철 (경상국립대학교 응용생명과학부(BK21Four))
  • Received : 2024.06.12
  • Accepted : 2024.06.24
  • Published : 2024.06.30
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Abstract

This study was conducted to estimate the effects of the forage process on rumen fermentation characteristics and greenhouse gas emissions of rye. Rye was grown at the Taeyoung Livestock farm and harvested at the heading stage. The harvested rye (5 kg) was sub-sampled for fresh forage, hay, and silage in triplicates. The sub-sampled rye was freeze-dried or air-dried for fresh forage or rye hay, respectively. For rye silage, the sub-sampled rye forage was ensiled into a 10 L mini bucket silo and stored for 90 days. For 72 h rumen incubation, each forage (0.3 g) was placed into the incubation bottle with the rumen mixture (30 mL) in quadruplicates. After the incubation, total gas was measured and sub-sampled for CO2 and CH4 analyses, and the bottle content was centrifuged for in vitro digestibilities of dry matter (IVDMD) and neutral detergent fiber (IVNDFD), and rumen fermentation characteristics. Silage had higher crude protein, crude ash, and acid detergent fiber concentrations than fresh forage and hay but lower non-fiber carbohydrates and relative feed value (p<0.05). And, silage had higher lactic acid bacteria than the other forages but lower pH (p<0.05). After 72 h incubation in the rumen, fresh forage had higher IVDMD and butyrate content than the other forages (p<0.05). However, silage had higher rumen pH and propionate content than the other forages but lower A:P ratio (p<0.05). Regarding greenhouse gases, silage had lowest total gas (mL/g DMD and NDFD) and CH4 (mL/g DMD and NDFD) emissions, while fresh forage had lowest CO2 (mL/g DMD) emission (p<0.05). Therefore, this study concluded that the ensiling process of rye can effectively mitigate greenhouse gas emissions of Hanwoo.

본 연구에서는 국내에서 이용되는 호밀의 조사료 가공방법에 따른 영양소 함량, 반추위 내 소화율, 발효특성 및 온실가스 발생량을 평가하기 위해 수행되었다. 출수기에 수확한 호밀은 청초, 건초 및 사일리지로 제조하여 시험에 이용하였다. 조단백질, 조회분 및 ADF 함량은 사일리지에서 가장 높았고, 청초에서 가장 낮았다. NFC 함량과 RFV는 청초에서 가장 높았고, 사일리지에서는 가장 낮았다. 한편 호밀 조사료를 72시간 동안 한우의 반추위 내에서 in vitro 발효하였을 때, IVDMD는 청초가 건초와 사일리지에 비해 높았으나, IVNDFD 소화율은 시험구간 차이가 없었다. 반추위 내 propionate 함량이 사일리지에서 가장 높았으며, 이로 인해 A:P ratio는 가장 낮았다. IVDMD 기준 total gas와 CH4 발생량은 사일리지가 가장 낮았으며, CO2 발생량은 생초가 낮았다. 한편 IVNDFD 기준 total gas, CO2 및 CH4 발생량은 사일리지가 가장 낮았다. 이상의 결과에서, 호밀은 사일리지로 제조하는 것이 온실가스 저감에서 유리할 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 농림식품기술기획평가원의 연구사업(과제번호: RS-2021-IP321083)의 지원에 의해 이루어졌습니다.

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