Journal of Ecology and Environment

한국생태학회 (The Ecological Society of Korea)
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Molecular identification of fruit bats, natural host of Nipah virus in Bangladesh, based on DNA barcode

  • Md. Maharub Hossain Fahim (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University) ;
  • Walid Hassan (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University) ;
  • Afia Afsin (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University) ;
  • Md. Mahfuzur Rahman (International Centre for Diarrhoeal Disease Research) ;
  • Md. Tanvir Rahman (Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Sang Jin Lim (College of Forest & Environmental Sciences, Kangwon National University) ;
  • Yeonsu Oh (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Yung Chul Park (College of Forest & Environmental Sciences, Kangwon National University) ;
  • Hossain Md. Faruquee (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University) ;
  • Md. Mafizur Rahman (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University)
  • 투고 : 2024.01.03
  • 심사 : 2024.03.04
  • 발행 : 2024.06.30
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초록

Background: Fruit bats are natural carriers of Nipah virus (NiV). The primary objective of this study is to identify potential reservoir species in a selected geographic regions. It is necessary to determine an accurate species identification of the associated reservoir bat species distributed in a specific region. Results: In this study, we collected 20 different bat specimens from the NiV-prone area of the Kushtia district. Among these, 14 were tissue samples (BT-1-14) and six were fecal samples (BF-1-6). We used the mitochondrial gene cytochrome b, one of the most abundant and frequently used genetic markers, for polymerase chain reaction amplification and sequencing. Out of the 20 samples, 12 tissue samples and 2 fecal samples were successfully amplified and sequenced. However, two tissue samples and four fecal samples yielded chimeric sequences, rendering them unsuitable for annotation. The sequences of the successfully amplified samples were compared to those deposited in the National Center for Biotechnology Information database using basic local alignment search tool to identify the bat specimen collected. The study identified six different bat species using both morphological and genetic data, which may carriers of the NiV. Conclusions: Our results suggest that additional research should be conducted to gather more information on fruit bats from different localities across the country. The study contributes to the establishment of appropriate measures for NiV carrying disease control and management.

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과제정보

We acknowledge the permission authority. We thank all of the studneds at Islamic University, Kushtia and Bangladesh Agricultural University, Mymensingh, for sample collection.

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