Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
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Ecological analysis and culture methods for freshwater green algae Prasiola japonica

  • Dong Sam Kim (Samcheok Prasiola Japonica Center) ;
  • Dong Seon Kim (Department of Integrative Biotechnology, Sungkyunkwan University) ;
  • Jae Youl Cho (Department of Integrative Biotechnology, Sungkyunkwan University)
  • Received : 2023.12.04
  • Accepted : 2024.02.19
  • Published : 2024.06.30
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Abstract

Background: Prasiola japonica is a freshwater green algae species that can only be seen in Korea and Japan. The various conditions necessary for its growth and reproduction have not been fully elucidated. Therefore, in this study, we aimed to investigate conditions related to the growth and reproduction of P. japonica for the purpose of conserving and producing this species. We first examined differences in growth according to various conditions in different habitats to understand the growth environment of P. japonica. Results: The experimental results revealed that the optimal temperature for growth and reproduction of P. japonica was between 10℃-15℃, and the optimal light intensity was 1,000-1,500 lux. Furthermore, when Provasoli enriched seawater with iodine (PESI) agar was used, the growth of P. japonica was found to be at least 1.5-8 times greater than that of the general Sohan Valley water sample, and it also showed 2-4.5 faster growth rate to reach 30 ㎛. These results emphasize the importance of PESI agar in the culture of P. japonica, and are expected to be helpful in suggesting ways to utilize and conserve P. japonica resources. Conclusions: Through these research findings, we suggest new methods for conserving and producing P. japonica, highlight the importance of preserving the P. japonica ecosystem, and explore ways to utilize P. japonica resources. This research promotes the understanding and protection of P. japonica resources in Korea and beyond, and underscores the need for further research and conservation efforts.

Keywords

Acknowledgement

This research was supported by Samcheok Prasiola Japonica Research Center, Samcheok city, Korea.

References

  1. Adenan NS, Yusoff FM, Shariff M. Effect of salinity and temperature on the growth of diatoms and green algae. J Fish Aquat Sci. 2013;8(2):397-404. https://doi.org/10.3923/jfas.2013.397.404. 
  2. Andersen RA. Algal culturing techniques. Burlington: Academic Press;2005. 
  3. Borowitzka MA. Algal physiology and large-scale outdoor cultures of microalgae. In: Borowitzka MA, Beardall J, Raven JA, editors. The physiology of microalgae. Cham: Springer; 2016. p. 601-52. 
  4. Choi SK, Kang YH, Park SR. Growth responses of kelp species Ecklonia cava to different temperatures and nitrogen sources. Korean J Environ Biol. 2020;38(3):404-15. https://doi.org/10.11626/KJEB.2020.38.3.404. 
  5. De Silva MWRN, Burrows EM. An experimental assessment of the status of the species Enteromorpha intestinalis (L.) Link and Enteromorpha compressa (L.) Grev. J Mar Biol Assoc U K. 1973;53(4):895-904. https://doi.org/10.1017/S0025315400022554. 
  6. Gao X, Endo H, Nagaki M, Agatsuma Y. Growth and survival of juvenile sporophytes of the kelp Ecklonia cava in response to different nitrogen and temperature regimes. Fish Sci. 2016;82(4):623-9. https://doi. org/10.1007/s12562-016-0998-4. 
  7. Goldman JC, Ryther JH, Williams LD. Mass production of marine algae in outdoor cultures. Nature. 1975;254(5501):594-5. https://doi.org/10.1038/254594a0. 
  8. Guiry MD, Guiry GM. AlgaeBase. 2008. https://www.algaebase.org. Accessed 1 Oct 2023. 
  9. Kim KY, Lee IK. The germling growth of Enteromorpha intestinalis (Chlorophyta) in laboratory culture under different combinations of irradiance and salinity and temperature and salinity. Phycologia. 1996;35(4):327-31. https://doi.org/10.2216/i0031-8884-35-4-327.1. 
  10. Kim MS, Jun MS, Kim CA, Yoon J, Kim JH, Cho GY. Morphology and phylogenetic position of a freshwater Prasiola species (Prasiolales, Chlorophyta) in Korea. Algae. 2015;30(3):197-205. https://doi.org/10.4490/algae.2015.30.3.197. 
  11. Kim S, Kang YH, Kim TH, Lee HJ, Park SR. Use of morphological characteristics for calculating individual biomass in the kelp Ecklonia cava. J Appl Phycol. 2017;29(5):2587-93. https://doi.org/10.1007/s10811-017-1140-4. 
  12. Kim SH, Kim YD, Hwang MS, Hwang EK, Yoo HI. Temperature ranges for survival and growth of juvenile Saccharina sculpera (Laminariales, Phaeophyta) and applications for field cultivation. Algae. 2021;36(4):231-40. https://doi.org/10.4490/algae.2021.36.11.21. 
  13. Ko SJ, Kim YK, Hong SW, Kang MS, Park CS, Hwang EK, et al. Artificial seed production and cultivation of Sargassum macrocarpum (Fucales, Phaeophyta). Algae. 2020;35(2):123-31. https://doi.org/10.4490/algae.2020.35.5.27. 
  14. Lee CY, Park SH, Lim HY, Jang SG, Park KJ, Kim DS, et al. In vivo anti-inflammatory effects of Prasiola japonica ethanol extract. J Funct Foods. 2021;80:104440. https://doi.org/10.1016/j.jff.2021.104440. 
  15. Lee KH, Jeong HJ, Kim HJ, Lim AS. Nitrate uptake of the red tide dinoflagellate Prorocentrum micans measured using a nutrient repletion method: effect of light intensity. Algae. 2017;32(2):139-53. https://doi.org/10.4490/algae.2017.32.5.20. 
  16. Naw MWD, Hara Y. Morphology and molecular phylogeny of Prasiola sp. (Prasiolales, Chlorophyta) from Myanmar. Phycol Res. 2002; 50(3):175-82. https://doi.org/10.1046/j.1440-1835.2002.00271.x. 
  17. Okada Y. On a new Prasiola from Corea. J Jpn Bot. 1938;15:449-52. 
  18. Park MK, Kim WK, Chung IS, Lee EB. Study on the Prasiola sp. in Korea (1) - ecological and morphological studies on the Prasiola sp. in the Samchuck - Chodang. J Plant Biol. 1970;13(2):1-10. 
  19. Park SH, Choi E, Kim S, Kim DS, Kim JH, Chang S, et al. Oxidative stress-protective and anti-melanogenic effects of loliolide and ethanol extract from fresh water green algae, Prasiola japonica. Int J Mol Sci. 2018;19(9):2825. https://doi.org/10.3390/ijms19092825. 
  20. Park SH, Kim JH, Song M, Lee HP, Yoon JH, Kim DS, et al. Chloroform fraction of Prasiola japonica ethanolic extract alleviates UPM 1648a-induced lung injury by suppressing NF-κB signaling. Foods. 2022;12(1):88. https://doi.org/10.3390/foods12010088. 
  21. Poppe F, Schmidt RAM, Hanelt D, Wiencke C. pEffects of UV radiation on the ultrastructure of several red algae. Phycol Res. 2003;51(1):11-9. https://doi.org/10.1046/j.1440-1835.2003.00288.x. 
  22. Pringle JD. Swarmer release and distribution of life-cycle phases of Enteromorpha intestinalis (L.) (Chlorophyta) in relation to environmental factors. J Exp Mar Biol Ecol. 1986;100(1-3):97-111. https://doi.org/10.1016/0022-0981(86)90157-7. 
  23. Rahmawati L, Park SH, Kim DS, Lee HP, Aziz N, Lee CY, et al. Anti-inflammatory activities of the ethanol extract of Prasiola japonica, an Edible freshwater green algae, and its various solvent fractions in LPS-induced macrophages and carrageenan-induced paw edema via the AP-1 pathway. Molecules. 2021;27(1):194. https://doi.org/10.3390/molecules27010194. 
  24. Sato Y, Kozono J, Nishihara GN, Terada R. Effect of light and temperature on photosynthesis of a cultivated brown alga, Saccharina sculpera (Laminariales), from Japan. Phycologia. 2020;59(4):375-84. https://doi.org/10.1080/00318884.2020.1777384. 
  25. Seo DW, Kim HJ, Jang SK, Jun M, Joo SS. Screening of functional components derived from fresh water laver, Prasiola japonica, and its pharmacological properties. J Biomed Res. 2013;14(2):83-90. 
  26. Serisawa Y, Yokohama Y, Aruga Y, Tanaka J. Growth of Ecklonia cava (Laminariales, Phaeophyta) sporophytes transplanted to a locality with different temperature conditions. Phycol Res. 2002;50(3):201-7. https://doi.org/10.1046/j.1440-1835.2002.00274.x. 
  27. Shen Y, Yuan W, Pei ZJ, Wu Q, Mao E. Microalgae mass production methods. Trans ASABE. 2009;52(4):1275-87. https://doi.org/10.13031/2013.27771. 
  28. Singh SP, Singh P. Effect of temperature and light on the growth of algae species: a review. Renew Sustain Energy Rev. 2015;50:431-44. https://doi.org/10.1016/j.rser.2015.05.024. 
  29. Sorokin C, Krauss RW. The effects of light intensity on the growth rates of green algae. Plant Physiol. 1958;33(2):109-13. https://doi.org/10.1104/pp.33.2.109. 
  30. Takeda H, Nisizawa K, Miwa T. Histochemical and chemical studies on the cell wall of Prasiola japonica. Bot Mag Tokyo. 1967;80:109-17. 
  31. Yoo HI, Lee KH, Kim SH, Ha DS, Hwang EK. Regeneration and the maturation induction of free-living gametophytes of a kelp Saccharina sculpera (Phaeophyceae). Korean J Environ Biol. 2018;36(4):576-83. https://doi.org/10.11626/KJEB.2018.36.4.576.