치위생과학회지 (Journal of dental hygiene science)

  • 제24권2호
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  • Pages.97-106
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  • 2024
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  • 1598-4478(pISSN)
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  • 2233-7679(eISSN)
한국치위생과학회 (The Korean Society of Dental Hygiene Science)
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Multiplex Real-Time Polymerase Chain Reaction Analysis of Pathogens in Peri-Implantitis and Periodontitis: A Randomized Trial

  • Eun-Deok Jo (Department of Dental Hygiene, College of Health Science, Gachon University)
  • 투고 : 2024.06.04
  • 심사 : 2024.06.17
  • 발행 : 2024.06.30
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초록

Background: Periodontitis and peri-implantitis are diseases caused by pathogenic microorganisms that cause tissue damage and alveolar bone destruction resulting in the loss of teeth and implants. Due to the biological differences in the tissues surrounding the implants, peri-implantitis progresses more rapidly and intensely than periodontitis, underscoring the importance of understanding the characteristics and interactions of pathogenic bacteria. This study aimed to quantitatively analyze the pathogenic microorganisms associated with periodontitis and peri-implantitis in Korean patients and evaluate the correlation between these bacteria. Methods: A total of 98 (52 males and 46 females) were randomly selected and classified into three groups (healthy group [HG]=25; periodontitis group [PG]=31; and peri-implantitis group [PIG]=42). The relative expression levels of 11 pathogenic microorganisms collected from the gingival sulcus fluid were determined using multiplex real-time polymerase chain reaction. Results: Eikenella corrodens, Fusobacterium nucleatum, and Prevotella nigrescens were highly prevalent in the HG, PG, and PIG patients. The results of the relative quantitative analysis of microorganisms showed that all bacteria belonging to the green, orange, and red complexes were significantly more abundant in the PG and PIG than in the HG (p<0.05). Porphyromonas gingivalis in the red complex showed a positive correlation with all microorganisms in the orange complex (p<0.05). Campylobacter rectus in the orange complex showed a significant positive correlation with all microorganisms in the red complex, and with F. nucleatum, P. nigrescens, Prevotella intermedia, and Eubacterium nodatum (p<0.05). Conclusion: P. gingivalis, C. rectus, and F. nucleatum exhibit strong interactions. Removing these bacteria can block complex formation and enhance the prevention and treatment of periodontitis and peri-implantitis.

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참고문헌

  1. Salvi GE, Aglietta M, Eick S, Sculean A, Lang NP, Ramseier CA: Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans. Clin Oral Implants Res 23: 182-190, 2012. https://doi.org/10.1111/j.1600-0501.2011.02220.x
  2. Hajishengallis G, Darveau RP, Curtis MA: The keystone-pathogen hypothesis. Nat Rev Microbiol 10: 717-725, 2012. https://doi.org/10.1038/nrmicro2873
  3. Colombo AP, Bennet S, Cotton SL, et al.: Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray. J Periodontol 83: 1279-1287, 2012. https://doi.org/10.1902/jop.2012.110566
  4. Al-Ahmad A, Wiedmann-Al-Ahmad M, Faust J, et al.: Biofilm formation and composition on different implant materials in vivo. J Biomed Mater Res B Appl Biomater 95: 101-109, 2010. https://doi.org/10.1002/jbm.b.31688
  5. Kumar PS, Mason MR, Brooker MR, O'Brien K: Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants. J Clin Periodontol 39: 425-433, 2012. https://doi.org/10.1111/j.1600-051x.2012.01856.x
  6. Heitz-Mayfield LJA, Lang NP: Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis. Periodontol 2000 53: 167-181, 2010. https://doi.org/10.1111/j.1600-0757.2010.00348.x
  7. Koyanagi T, Sakamoto M, Takeuchi Y, Maruyama N, Ohkuma M, Izumi Y: Comprehensive microbiological findings in peri-implantitis and periodontitis. J Clin Periodontol 40: 218-226, 2013. https://doi.org/10.1111/jcpe.12047
  8. Torrungruang K, Jitpakdeebordin S, Charatkulangkun O, Gleebbua Y: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola / Prevotella intermedia co-infection are associated with severe periodontitis in a Thai population. PLoS One 10: e0136646, 2015. https://doi.org/10.1371/journal.pone.0136646
  9. Iusan SAL, Lucaciu OP, Petrescu NB, et al.: The main bacterial communities identified in the sites affected by periimplantitis: a systematic review. Microorganisms 10: 1232, 2022. https://doi.org/10.3390/microorganisms10061232
  10. Hultin M, Gustafsson A, Hallstrom H, Johansson LA, Ekfeldt A, Klinge B: Microbiological findings and host response in patients with peri-implantitis. Clin Oral Implants Res 13: 349-358, 2002. https://doi.org/10.1034/j.1600-0501.2002.130402.x
  11. Persson GR, Renvert S: Cluster of bacteria associated with peri-implantitis. Clin Implant Dent Relat Res 16: 783-793, 2014. https://doi.org/10.1111/cid.12052
  12. Teles R, Teles F, Frias-Lopez J, Paster B, Haffajee A: Lessons learned and unlearned in periodontal microbiology. Periodontol 2000 62: 95-162, 2013. https://doi.org/10.1111/prd.12010
  13. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr: Microbial complexes in subgingival plaque. J Clin Periodontol 25: 134-144, 1998. https://doi.org/10.1111/j.1600-051x.1998.tb02419.x
  14. Gambin DJ, Vitali FC, De Carli JP, et al.: Prevalence of red and orange microbial complexes in endodontic-periodontal lesions: a systematic review and meta-analysis. Clin Oral Investig 25: 6533-6546, 2021. https://doi.org/10.1007/s00784-021-04164-4
  15. Socransky SS, Haffajee AD: The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63: 322-331, 1992. https://doi.org/10.1902/jop.1992.63.4s.322
  16. Sculean A, Deppe H, Miron R, Schwarz F, Romanos G, Cosgarea R: Effectiveness of photodynamic therapy in the treatment of periodontal and peri-implant diseases. Oral Biofilms 29: 133-143, 2021. https://doi.org/10.1159/000510189
  17. Gersdorf H, Meissner A, Pelz K, Krekeler G, Gobel UB: Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J Clin Microbiol 31: 941-946, 1993. https://doi.org/10.1128/jcm.31.4.941-946.1993
  18. Luo L, Xie P, Gong P, Tang XH, Ding Y, Deng LX: Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis patients and peri-implantitis. Arch Oral Biol 56: 1106-1111, 2011. https://doi.org/10.1016/j.archoralbio.2011.03.020
  19. Kim HS, Lim SA: Comparison between bacterial culture method and multiplex PCR for identification of Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans from the dental plaques. J Dent Hyg Sci 9: 249-255, 2009.
  20. World Medical Association: World Medical Association declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 310: 2191-2194, 2013. https://doi.org/10.1001/jama.2013.281053
  21. Lang NP, Berglundh T; Working Group 4 of Seventh European Workshop on Periodontology: Periimplant diseases: where are we now?--Consensus of the seventh European workshop on periodontology. J Clin Periodontol 38: 178-181, 2011. https://doi.org/10.1111/j.1600-051x.2010.01674.x
  22. Rajasekar A, Varghese SS: Microbiological profile in periodontitis and peri-implantitis: a systematic review. J Long Term Eff Med Implants 32: 83-94, 2022. https://doi.org/10.1615/jlongtermeffmedimplants.2022043121
  23. Field CA, Gidley MD, Preshaw PM, Jakubovics N: Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes. J Periodontal Res 47: 470-478, 2012. https://doi.org/10.1111/j.1600-0765.2011.01455.x
  24. Griffen AL, Beall CJ, Campbell JH, et al.: Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing. ISME J 6: 1176-1185, 2012. https://doi.org/10.1038/ismej.2011.191
  25. Maeda N, Okamoto M, Kondo K, et al.: Incidence of Prevotella intermedia and Prevotella nigrescens in periodontal health and disease. Microbiol Immunol 42: 583-589, 1998. https://doi.org/10.1111/j.1348-0421.1998.tb02328.x
  26. Teanpaisan R, Douglas CW, Walsh TF: Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites. J Periodontal Res 30: 245-251, 1995. https://doi.org/10.1111/j.1600-0765.1995.tb02129.x
  27. Kim MJ, Han GS: Quantitative detection of peri-implantitis bacteria using real-time PCR. J Korean Soc Dent Hyg 21: 555-565, 2021. https://doi.org/10.13065/jksdh.20210053
  28. Pinto G, Silva MD, Peddey M, Sillankorva S, Azeredo J: The role of bacteriophages in periodontal health and disease. Future Microbiol 11: 1359-1369, 2016. https://doi.org/10.2217/fmb-2016-0081
  29. Harvey JD: Periodontal microbiology. Dent Clin North Am 61: 253-269, 2017. https://doi.org/10.1016/j.cden.2016.11.005
  30. Canullo L, Penarrocha-Oltra D, Covani U, Botticelli D, Serino G, Penarrocha M: Clinical and microbiological findings in patients with peri-implantitis: a cross-sectional study. Clin Oral Implants Res 27: 376-382, 2016. https://doi.org/10.1111/clr.12557
  31. Wang HL, Garaicoa-Pazmino C, Collins A, Ong HS, Chudri R, Giannobile WV: Protein biomarkers and microbial profiles in peri-implantitis. Clin Oral Implants Res 27: 1129-1136, 2016. https://doi.org/10.1111/clr.12708
  32. Tamura N, Ochi M, Miyakawa H, Nakazawa F: Analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16S rDNA gene sequence. Int J Oral Maxillofac Implants 28: 1521-1529, 2013. https://doi.org/10.11607/jomi.2570
  33. Ebadian AR, Kadkhodazadeh M, Zarnegarnia P, Dahlen G: Bacterial analysis of peri-implantitis and chronic periodontitis in Iranian subjects. Acta Med Iran 50: 486-492, 2012.
  34. Dabdoub SM, Tsigarida AA, Kumar PS: Patient-specific analysis of periodontal and peri-implant microbiomes. J Dent Res 92(12 Suppl): 168S-175S, 2013. https://doi.org/10.1177/0022034513504950
  35. Paul Rosen DC, David Cochran, Stuart Froum, Bradley McAllister, Stefan Renvert, Hom Lay Wang: Peri-implant mucositis and peri-implantitis: a current understanding of their diagnoses and clinical implications. J Periodontol 84: 436-443, 2013. https://doi.org/10.1902/jop.2013.134001
  36. Park KH, Han GS: The effects of professional tooth cleaning and plaque control instruction on reduction of peri-implantitis. J Dent Hyg Sci 12: 163-170, 2012.
  37. Kim YS, Oh MJ: The effect of following oral health care on implant patients. J Dent Hyg Sci 9: 491-496, 2009.