Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Changes of Sensitivity to Streptomycin in Erwinia amylovora Isolated from 2019 to 2023 in Korea

2019-2023년 국내에서 분리한 Erwinia amylovora의 스트렙토마이신에 대한 감수성 변화

  • Hyeonheui Ham (Department of Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Ga-Ram Oh (Department of Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Bang Wool Lee (Department of Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Yong Hwan Lee (Department of Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Yong Hoon Lee (Division of Biotechnology, Jeonbuk National University)
  • 함현희 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 오가람 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 이방울 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 이용환 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 이용훈 (전북대학교 생명공학부)
  • Received : 2024.04.26
  • Accepted : 2024.06.13
  • Published : 2024.06.30
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Abstract

Erwinia amylovora, a causal pathogen of fire blight, has been continuously inducing damage to the apple and pear trees in South Korea since 2015. Farmers apply antibiotics during blooming season to prevent the fire blight. However, continuous use of antibiotics can induce the emergence of resistant bacteria, which consequently reduces control efficacy. In this study, we assessed the minimal inhibitory concentration (MIC) of streptomycin, using a total of 361 E. amylovora isolates that were collected from the six provinces of South Korea from 2019 to 2023. As a result, the MIC of streptomycin ranged from 0.5 to 4 ㎍/ml and the strA-strB genes were not identified from the isolates. The MIC was higher in the isolates from Gyeonggi-do, Gangwondo, and Chungcheongbuk-do compared to those from other three provinces. These results may bring broad attention to the use of streptomycin and aid in developing a management protocol for the occurrence of fire blight in South Korea.

Erwinia amylovora는화상병의원인균으로국내에서는 2015년 이후 사과와 배 나무에 큰 피해를 일으키고 있다. 농가에서는 화상병 예방을 위해 개화기에 항생제를 살포하고 있다. 그러나 항생제의 지속적인 사용은 저항성균의 출현을 유발하고, 이로 인해 방제 효율의 저하를 초래할 수 있다. 따라서, 본 연구에서는 2019–2023년 동안 국내에서 분리한 E. amylovora 361균주를 대상으로 스트렙토마이신에 대한 최소억제농도의 변화 양상을 조사하였다. 국내에서 분리한 연구 대상 균주들에 대한 스트렙토마이신의 최소억제농도는 0.5–4 µg/ml였고 균주에서 스트렙토마이신에 약한 저항성을 유도하는 strA-strB 유전자는 확인되지 않았다. 또한 경기도, 강원도 및 충청북도에서 다른 지역에 비해 스트렙토마이신의 최소억제농도가 높았다. 이러한 연구 결과는 스트렙토마이신의 사용에 대한 주의를 유도하고 방제 대책을 수립하기 위한 기초자료로 활용될 수 있을 것이다.

Keywords

Acknowledgement

This research was supported by a Cooperative Research Program (Project no. RS-2020-RD009337) from Rural Development Administration, Republic of Korea.

References

  1. Acimovic, S. G. and Meredith, C. L. 2017. Evaluation of dormant copper sprays with bark penetrating surfactants in reduction of Erwinia amylovora in cankers and of low-rate copper sprays in blossom blight control. Fruit Q. 25: 15-20.
  2. Brauner, A., Fridman, O., Gefen, O. and Balaban, N. Q. 2016. Distinguishing between resistance, tolerance and persistence to antibiotic treatment. Nat. Rev. Microbiol. 14: 320-330.
  3. Chang, Q., Wang, W., Regev-Yochay, G., Lipsitch, M. and Hanage, W. P. 2015. Antibiotics in agriculture and the risk to human health: how worried should we be? Evol. Appl. 8: 240-247.
  4. Chiou, C.-S. and Jones, A. L. 1995. Molecular analysis of high-level streptomycin resistance in Erwinia amylovora. Phytopathology 85: 324-328.
  5. Cui, Z., Huntley, R. B., Zeng, Q. and Steven, B. 2021. Temporal and spatial dynamics in the apple flower microbiome in the presence of the phytopathogen Erwinia amylovora. ISME J. 15: 318-329.
  6. DuPont, S. T., Cox, K., Johnson, K., Peter, K., Smith, T., Munir, M. et al. 2023. Evaluation of biopesticides for the control of Erwinia amylovora in apple and pear. J. Plant Pathol. Online publication. https://doi.org/10.1007/s42161-023-01372-7.
  7. Forster, H., McGhee, G. C., Sundin, G. W. and Adaskaveg, J. E. 2015. Characterization of streptomycin resistance in isolates of Erwinia amylovora in California. Phytopathology 105: 1302-1310.
  8. Ham, H., Lee, K. J., Hong, S. J., Kong, H. G., Lee, M.-H., Kim, H.-R. et al. 2020. Outbreak of fire blight of apple and pear and its characteristics in Korea in 2019. Res. Plant Dis. 26: 239-249. (In Korean)
  9. Ham, H., Oh, G.-R., Lee, B. W., Lee, Y. H. and Lee, Y. H. 2023. Assessment of oxytetracycline and oxolinic acid resistance of Erwinia amylovora isolated from 2019 to 2022 in Korea. Korean J. Pestic. Sci. 27: 283-292. (In Korean)
  10. Ham, H., Oh, G.-R., Park, D. S. and Lee, Y. H. 2022. Survey of oxolinic acid-resistant Erwinia amylovora in Korean apple and pear orchards, and the fitness impact of constructed mutants. Plant Pathol J. 38: 482-489.
  11. Johnson, K. B., Smith, T. J., Temple, T. N., Gutierrez, E., Elkins, R. B. and Castagnoli, S. P. 2016. Integration of acibenzolar-S-methyl with antibiotics for protection of pear and apple from fire blight caused by Erwinia amylovora. Crop Prot. 88: 149-154.
  12. Jones, A. L. and Schnabel, E. L. 2000. The development of streptomycin-resistant strains of Erwinia amylovora. CABI Publishing, Wallingford, UK. 235-251 pp.
  13. Lee, M. S., Lee, I., Kim, S. K., Oh, C.-S. and Park, D. H. 2018. In vitro screening of antibacterial agents for suppression of fire blight disease in Korea. Res. Plant Dis. 24: 41-51. (In Korean)
  14. McGhee, G. C., Guasco, J., Bellomo, L. M., Blumer-Schuette, S. E., Shane, W. W., Irish-Brown, A. et al. 2011. Genetic analysis of streptomycin-resistant (SmR) strains of Erwinia amylovora suggests that dissemination of two genotypes is responsible for the current distribution of SmR E. amylovora in Michigan. Phytopathology 101: 182-191.
  15. McGhee, G. C. and Sundin, G. W. 2011. Evaluation of kasugamycin for fire blight management, effect on nontarget bacteria, and assessment of kasugamycin resistance potential in Erwinia amylovora. Phytopathology 101: 192-204.
  16. McManus, P. S. and Jones, A. L. 1994. Epidemiology and genetic analysis of streptomycin-resistant Erwinia amylovora from Michigan and evaluation of oxytetracycline for control. Phytopathology 84: 627-633.
  17. McManus, P. S., Stockwell, V. O., Sundin, G. W. and Jones, A. L. 2002. Antibiotic use in plant agriculture. Annu. Rev. Phytopathol. 40: 443-465.
  18. Miller, T. D. and Schroth, M. N. 1972. Monitoring the epiphytic population of Erwinia amylovora. Phytopathology 62: 1175-1182.
  19. Palmer, E. L., Teviotdale, B. L. and Jones, A. L. 1997. A relative of the broad-host-range plasmid RSF1010 detected in Erwinia amylovora. Appl. Environ. Microbiol. 63: 4604-4607.
  20. Pedroncelli, A. and Puopolo, G. 2023. This tree is on fire: a review on the ecology of Erwinia amylovora, the causal agent of fire blight disease. J. Plant Pathol. Online publication. https://doi.org/10.1007/s42161-023-01397-y.
  21. Shrestha, R., Lee, S. H., Hur, J. H. and Lim, C. K. 2005. The effects of temperature, pH, and bactericides on the growth of Erwinia pyrifoliae and Erwinia amylovora. Plant Pathol J. 21: 127-131.
  22. Spickler, C., Brunelle, M.-N. and Brakier-Gingras, L. 1997. Streptomycin binds to the decoding center of 16 S ribosomal RNA. J. Mol. Biol. 273: 586-599.
  23. Stockwell, V. O. and Duffy, B. 2012. Use of antibiotics in plant agriculture. Rev. Sci. Tech. 31: 199-210.
  24. Sundin, G. W. and Wang, N. 2018. Antibiotic resistance in plantpathogenic bacteria. Annu. Rev. Phytopathol. 56: 161-180.
  25. Thomson, S. V. 1986. The role of the stigma in fire blight infections. Phytopathology 76: 476-482.
  26. Van der Zwet, T., Orolaza-Halbrendt, N. and Zeller, W. 2012. Fire blight : history, biology, and management. APS Press, St. Paul, MN, USA. 421 pp.
  27. Vanneste, J. L. 2000. Fire blight: the disease and its causative agent, Erwinia amylovora. CABI Publishing, New York, NY, USA.
  28. Wiegand, I., Hilpert, K. and Hancock, R. E. 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3: 163-175.
  29. Yuan, X., Gdanetz, K., Outwater, C. A., Slack, S. M. and Sundin, G. W. 2023. Evaluation of plant defense inducers and plant growth regulators for fire blight management using transcriptome studies and field assessments. Phytopathology 113: 2152-2164.