식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

멸종위기종 구상나무 서식지별 세균 군집 구조 변이 조사

Investigation of Variation in Bacterial Community Structure in Endangered Korean Fir Tree by Habitats

  • 고영민 (경상대학교 식물의학과) ;
  • 강근혜 (국립공원야생생물보전원) ;
  • 정대호 (국립공원야생생물보전원) ;
  • 곽연식 (경상대학교 식물의학과)
  • Young Min Ko (Department of Plant Medicine, Gyeongsang National University) ;
  • Geun-Hye Gang (National Park Institute for Wildlife Conservation) ;
  • Dae Ho Jung (National Park Institute for Wildlife Conservation) ;
  • Youn-Sig Kwak (Department of Plant Medicine, Gyeongsang National University)
  • 투고 : 2024.03.09
  • 심사 : 2024.04.03
  • 발행 : 2024.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

구상나무(Abies koreana)는 한국의 고유종이지만 기후 변화로 인한 심각한 개체수 감소 현상이 일어나고 있다. 구상나무의 보전을 위한 다양한 연구가 기존에 진행되었지만 구상나무와 근권 세균 군집의 상관관계에 대한 연구는 비교적 미진한 편이기 때문에 추가적인 연구 진행이 필요하다. 식물의 근권 미생물 군집을 구성하는 일부 세균은 식물이 수분, 양분 등이 부족한 환경에서 살아남을 수 있게 돕는다. 또한 계절 변화에 의한 급격한 환경 변화에 대한 식물의 적응력을 향상시켜 줄 가능성이 있다. 본 연구에서는 이러한 세균 군집이 구상나무의 근권에 존재하거나 구상나무의 서식지 유형(자생지, 대체서식지, 고사지, 비재배토양)과 계절 변화(4월, 6월, 9월, 11월)에 따른 군집 구조의 차이가 발생하는지 파악하기 위해 세균의 16S rRNA V4 영역을 대상으로 한 metagenome amplicon sequencing을 진행하였다. 그 결과 우리는 구상나무와 매우 특이적으로 상호작용하는 근권 세균 군집이나 특정 분류군은 찾을 수 없었지만 구상나무 비재배토양에 비해서 구상나무 자생지의 근권 세균 군집이 계절 변화에 의한 변동성이 적게 나타난다는 사실을 알아냈다. 이러한 결과는 구상나무의 근권의 환경이 비재배토양에 비해 상대적으로 안정하다는 것을 보여준다. 그러나 구상나무와 명확하게 상호작용하는 세균 군집이나 분류군을 찾을 수 없었기 때문에 세균 군집 이외의 진균과 같은 기타 근권 미생물 군집들에 대한 추가 연구의 필요성이 제시된다.

The Korean fir tree (Abies koreana), an endemic species of South Korea, is experiencing a severe decline in population due to climate change. Studies on the conservation of Korean fir have been extensive, yet research regarding its correlation with rhizosphere bacterial communities remains scarce, warranting further investigation. In this study, metagenome amplicon sequencing targeting the 16S rRNA V4 region was conducted to examine the presence of specific bacterial communities in Korean fir and to investigate potential differences based on habitat types (rhizosphere of native or cultivated trees, soil of dead trees, and bulk soil) and seasonal variations (April, June, September, November). Here we show that although we could not identify specific taxa highly specifically with Korean fir, the rhizosphere bacterial community in native trees exhibited less variability in response to seasonal changes compared to that in bulk soils. Suggesting the establishment of relatively stable bacterial populations around the Korean fir natural habitat. Further research on other types of rhizosphere and/or microbes is necessary to investigate the distinct relationship of Korean fir with microbial communities.

키워드

과제정보

This research was supported by the National Park Institute for Wildlife Conservation.

참고문헌

  1. Ahn, U. S. and Yun, Y. S. 2020. Causes of decline in the Korean fir based on spatial distribution in the Mt. Halla region in Korea: a meta-analysis. Forests 11: 391.
  2. Alawiye, T. T. and Babalola, O. O. 2019. Bacterial diversity and community structure in typical plant rhizosphere. Diversity 11: 179.
  3. Anderson, M. J. 2017. Permutational multivariate analysis of variance (PERMANOVA). In: Wiley StatsRef: Statistics Reference Online, eds. by N. Balakrishnan, T. Colton, B. Everitt, W. Piegorsch, F. Ruggeri, J. L. Teugels, pp. 1-15. John Wiley and Sons Ltd., Chichester, UK.
  4. Bei, Q., Moser, G., Muller, C. and Liesack, W. 2021. Seasonality affects function and complexity but not diversity of the rhizosphere microbiome in European temperate grassland. Sci. Total Environ. 784: 147036.
  5. Burke, D. J., Kretzer, A. M., Rygiewicz, P. T. and Topa, M. A. 2006. Soil bacterial diversity in a loblolly pine plantation: influence of ectomycorrhizas and fertilization. FEMS Microbiol. Ecol. 57: 409-419.
  6. Chaparro, J. M., Badri, D. V. and Vivanco, J. M. 2014. Rhizosphere microbiome assemblage is affected by plant development. ISME J. 8: 790-803.
  7. Cho, G., Gang, G. H., Jung, H. Y. and Kwak, Y. S. 2022. Exploration of mycobiota in Cypripedium japonicum, an endangered species. Mycobiology 50: 142-149.
  8. Dawson, W., Hor, J., Egert, M., van Kleunen, M. and Pester, M. 2017. A small number of low-abundance bacteria dominate plant species-specific responses during rhizosphere colonization. Front. Microbiol. 8: 975.
  9. Dohrmann, A. B., Kuting, M., Junemann, S., Jaenicke, S., Schluter, A. and Tebbe, C. C. 2013. Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties. ISME J. 7: 37-49.
  10. Gwon, J. H., Sin, M. K., Kwon, H. J. and Song, H. K. 2013. A study on the forest vegetation of Jirisan National Park. J. Korean Soc. Environ. Rest. Technol. 16: 93-118.
  11. Han, G., Mannaa, M., Jeon, H., Jung, H., Kim, J. C., Park, A. R. et al. 2022. Dysbiosis in the rhizosphere microbiome of standing dead Korean fir (Abies koreana). Plants (Basel) 11: 990.
  12. Hawes, M. C., Bengough, G., Cassab, G. and Ponce, G. 2002. Root caps and rhizosphere. J. Plant Growth Regul. 21: 352-367.
  13. Hunter, P. R. and Gaston, M. A. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26: 2465-2466.
  14. Jeong, M., Tagele, S. B., Kim, M. J., Ko, S. H., Kim, K. S., Koh, J. G. et al. 2023. The death of Korean fir (Abies koreana) affects soil symbiotic fungal microbiome: preliminary findings. Front. For. Glob. Change 5: 1114390.
  15. Ji, R. Q., Xie, M. L., Li, G. L., Xu, Y., Gao, T. T., Xing, P. J. et al. 2022. Response of bacterial community structure to different ecological niches and their functions in Korean pine forests. PeerJ 10: e12978.
  16. Kim, H. S., Lee, S. M. and Song, H. K. 2011. Actual vegetation distribution status and ecological succession in the Deogyusan National Park. Korean J. Environ. Ecol. 25: 37-46.
  17. Latif, S., Bibi, S., Kouser, R., Fatimah, H., Farooq, S., Naseer, S. et al. 2020. Characterization of bacterial community structure in the rhizosphere of Triticum aestivum L. Genomics 112: 4760-4768.
  18. Lee, J. H. and Hong, S. K. 2009. Comparative analysis of chemical compositions and antimicrobial activities of essential oils from Abies holophylla and Abies koreana. J. Microbiol. Biotechnol. 19: 372-377.
  19. Liu, G. Y., Chen, L. L., Shi, X. R., Yuan, Z. Y., Yuan, L. Y., Lock, T. R. et al. 2019. Changes in rhizosphere bacterial and fungal community composition with vegetation restoration in planted forests. Land Degrad. Dev. 30: 1147-1157.
  20. Llado, S., Lopez-Mondejar, R. and Baldrian, P. 2017. Forest soil bacteria: diversity, involvement in ecosystem processes, and response to global change. Microbiol. Mol. Biol. Rev. 81: e00063-16.
  21. Mannaa, M., Han, G., Jung, H., Park, J., Kim, J. C., Park, A. R. et al. 2023. Aureobasidium pullulans treatment mitigates drought stress in Abies koreana via rhizosphere microbiome modulation. Plants (Basel) 12: 3653.
  22. Oppenheimer-Shaanan, Y., Jakoby, G., Starr, M. L., Karliner, R., Eilon, G., Itkin, M. et al. 2022. A dynamic rhizosphere interplay between tree roots and soil bacteria under drought stress. eLife 11: e79679.
  23. Phillips, R. P. and Fahey, T. J. 2006. Tree species and mycorrhizal associations influence the magnitude of rhizosphere effects. Ecology 87: 1302-1313.
  24. Prashar, P., Kapoor, N. and Sachdeva, S. 2014. Rhizosphere: its structure, bacterial diversity and significance. Rev. Environ. Sci. and Bio/Technol. 13: 63-77.
  25. Shannon, C. E. 1948. A mathematical theory of communication. Bell Syst. Tech. J. 27: 379-423.
  26. Smalla, K., Sessitsch, A. and Hartmann, A. 2006. The rhizosphere: 'soil compartment influenced by the root'. FEMS Microbiol. Ecol. 56: 165.
  27. Song, J. H., Han, S. H., Lee, S. H. and Yun, C. W. 2021. Ecological characteristic of Abies koreana stand structure of Mt. Jirisan and Mt. Hallasan. J. Korean Soc. Forest Sci. 110: 590-600.
  28. Wilson, E. H. 1920. Four new conifers from Korea. J. Arnold Arbor. 1: 186-190.
  29. Xu, J., Zhang, Y., Zhang, P., Trivedi, P., Riera, N., Wang, Y. et al. 2018. The structure and function of the global citrus rhizosphere microbiome. Nature Commun. 9: 4894.
  30. Xu, T., Shen, Y., Ding, Z. and Zhu, B. 2023. Seasonal dynamics of microbial communities in rhizosphere and bulk soils of two temperate forests. Rhizosphere 25: 100673.
  31. Yang, J., Kloepper, J. W. and Ryu, C. M. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci. 14: 1-4.