Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Biological and Molecular Characterization of a Korean Isolate of Clover Yellow Vein Virus Infecting Canavalia ensiformis

  • Bong-Geun Oh (Department of Agricultural Biology, Jeonbuk National University) ;
  • Ho-Jong Ju (Department of Agricultural Biology, Jeonbuk National University) ;
  • Jong-Sang Chung (B.I.G. Co., Ltd) ;
  • Ju-Yeon Yoon (Department of Plant Protection and Quarantine, Jeonbuk National University)
  • Received : 2024.05.14
  • Accepted : 2024.06.10
  • Published : 2024.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Jack bean (Canavalia ensiformis) is one of healthy products for fermented or functional food in Korea and is widely distributed and cultivated worldwide. During August 2022, Jack bean plants showing symptoms of yellow flecks, chlorosis, necrotic spots and mosaic were observed in Jangheung-gun, South Korea. By transmission electron microscopy, flexuous filamentous virus particles of approximately 750×13 nm in size were observed in the symptomatic leaf samples. The infection of a Korean isolate of clover yellow vein virus (ClYVV-Ce-JH) was confirmed using double antibody sandwich enzyme-linked sorbent assay, reverse transcription polymerase chain reaction and high-throughput sequencing. The complete genome sequence of ClYVV-Ce-JH consists of 9,549 nucleotides (nt) excluding the poly (A) tail and encodes 3,072 amino acids (aa), with an AUG start and UAG stop codon, containing one open reading frame that is typical of a potyvirus polyprotein. The polyprotein of ClYVV-Ce-JH was divided into ten proteins and each protein's cleavage sites were determined. The coat protein (CP) and polyprotein of ClYVV-Ce-JH were compared at the nt and aa levels with those of the previously reported 14 ClYVV isolates. ClYVV-Ce-JH shared 92.62% to 99.63% and 93.39% to 98.05% at the CP and polyprotein homology. To our knowledge, this is the first report of a Korean isolate of ClYVV from Jack bean plants and the complete genome sequence of a ClYVV Jack bean isolate in the world.

Keywords

Acknowledgement

This study was supported by "Research Base Construction Fund Support Program (no. 20230001470)" funded by Jeonbuk National University in 2023.

References

  1. Barnett, O. W., Randles, J. W. and Burrows, P. M. 1987. Relationships among Australian and North American isolates of the bean yellow mosaic potyvirus subgroup. Phytopathology 77: 791-799.  https://doi.org/10.1094/Phyto-77-791
  2. Bos, L., Lindsten, K. and Maat, D. Z. 1977. Similarity of clover yellow vein virus and Pea necrosis virus. Neth. J. Plant Pathol. 83: 97-108.  https://doi.org/10.1007/BF01981555
  3. CABI. 2000. Clover yellow vein potyvirus. Distribution maps of plant diseases. CAB International, Wallingford, UK, No. 811. 
  4. Chang, C.-A., Hiebert, E. and Purcifull, D. E. 1988a. Analysis of in vitro translation of bean yellow mosaic virus RNA: inhibition of proteolytic processing by antiserum to the 49K nuclear inclusion protein. J. Gen. Virol. 69: 1117-1122.  https://doi.org/10.1099/0022-1317-69-5-1117
  5. Chang, C.-A., Hiebert, E. and Purcifull, D. E. 1988b. Purification, characterization, and immunological analysis of nuclear inclusions induced by bean yellow mosaic and clover yellow vein potyviruses. Phytopathology 78: 1266-1275.  https://doi.org/10.1094/Phyto-78-1266
  6. Chung, B. Y.-W., Miller, W. A., Atkins, J. F. and Firth, A. E. 2008. An overlapping essential gene in the Potyviridae. Proc. Natl. Acad. Sci. U S A 105: 5897-5902.  https://doi.org/10.1073/pnas.0800468105
  7. Crnov, R. and Gilbertson, R. L. 2001. Outbreak of clover yellow vein virus in a bean field in Colusa County, California. Plant Dis. 85:444. 
  8. Dizadji, A. and Shahraeen, N. 2011. Occurrence, distribution and seasonal changes of viruses infecting common bean in northwestern Iran. Arch. Phytopathol. Plant Prot. 44: 1647-1654.  https://doi.org/10.1080/03235400903460627
  9. Hammond, J. and Hammond, R. W. 2003. The complete nucleotide sequence of isolate BYMV-GDD of bean yellow mosaic virus, and comparison to other potyviruses. Arch. Virol. 148: 2461-2470.  https://doi.org/10.1007/s00705-003-0185-7
  10. Hisa, Y., Suzuki, H., Atsumi, G., Choi, S. H., Nakahara, K. S. and Uyeda, I. 2014. P3N-PIPO of clover yellow vein virus exacerbates symptoms in pea infected with white clover mosaic virus and is implicated in viral synergism. Virology 449: 200-206.  https://doi.org/10.1016/j.virol.2013.11.016
  11. Hollings, M. and Nariani, T. K. 1965. Some properties of clover yellow vein, a virus from Trifolium repens L. Ann. Appl. Biol. 56: 99-109.  https://doi.org/10.1111/j.1744-7348.1965.tb01219.x
  12. Jo, Y., Choi, H., Lee, J. H., Moh, S. H. and Cho, W. K. 2022. Viromes of 15 pepper (Capsicum annuum L.) cultivars. Int. J. Mol. Sci. 23:10507. 
  13. Jones, R. T. and Diachun, S. 1977. Serologically and biologically distinct bean yellow mosaic virus strains. Phytopathology 67: 831-838.  https://doi.org/10.1094/Phyto-67-831
  14. Larsen, R. C., Miklas, P. N., Eastwell, K. C. and Grau, C. R. 2008. A strain of Clover yellow vein virus that causes severe pod necrosis disease in snap bean. Plant Dis. 92: 1026-1032.  https://doi.org/10.1094/PDIS-92-7-1026
  15. Li, Z., Xu, L., Sun, P., Zhu, M., Zhang, L., Zhang, B. et al. 2023. Biological and molecular characterization of clover yellow vein virus infecting Trifolium repens in China. Agronomy 13: 1193. 
  16. Ortiz, V., Castro, S. and Romero, J. 2009. First report of clover yellow vein virus in grain legumes in Spain. Plant Dis. 93: 106. 
  17. Park, C.-Y., Lee, M.-A., Nam, M., Park, E.-H., Bae, Y.-S., Lee, S.-H. et al. 2014. First report of clover yellow vein virus on white clover (Trifolium repens) in South Korea. Plant Dis. 98: 1450. 
  18. Park, I. S., Kim, K. W., Kyun, H. J. and Chang, M. U. 1998. The viruses in Gladiolus hybridus cultivated in Korea 1. Bean yellow mosaic virus and clover yellow vein virus. Korean J. Plant Pathol. 14: 74-82. (In Korean) 
  19. Parrella, G. and Lanave, C. 2009. Identification of a new pathotype of Bean yellow mosaic virus (BYMV) infecting blue passion flower and some evolutionary characteristics of BYMV. Arch. Virol. 154: 1689-1694.  https://doi.org/10.1007/s00705-009-0485-7
  20. Provvidenti, R. and Schroeder, W. T. 1973. Resistance in Phaseolus vulgaris to the severe strain of bean yellow mosaic virus. Phytopathology 63: 196-197.  https://doi.org/10.1094/Phyto-63-196
  21. Sasaya, T., Shimizu, T., Nozu, Y., Nishiguchi, M., Inouye, N. and Koganezawa, H. 1997. Biological, serological, and molecular variabilities of clover yellow vein virus. Phytopathology 87: 1014-1019.  https://doi.org/10.1094/PHYTO.1997.87.10.1014
  22. Shin, J.-C., Kim, M.-K., Kwak, H.-R., Choi, H.-S., Kim, J.-S., Park, C.-Y. et al. 2014. First report of clover yellow vein virus on Glycine max in Korea. Plant Dis. 98: 1283. 
  23. Takahashi, T., Uyeda, I., Ohshima, K. and Shikata, E. 1990. Nucleotide sequence of the capsid protein gene of bean yellow mosaic virus chlorotic spot strain. J. Fac. Agr. Hokkaido Univ. 64: 152-163. 
  24. Takahashi, Y., Takahashi, T. and Uyeda, I. 1997. A cDNA clone to clover yellow vein potyvirus genome is highly infectious. Virus Genes 14: 235-243.  https://doi.org/10.1023/A:1007940028058
  25. Tamura, K., Stecher, G. and Kumar, S. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Mol. Biol. Evol. 38: 3022-3027.  https://doi.org/10.1093/molbev/msab120
  26. Tracy, S. L., Frenkel, M. J., Gough, K. H., Hanna, P. J. and Shukla, D. D. 1992. Bean yellow mosaic, clover yellow vein, and pea mosaic are distinct potyviruses: evidence from coat protein gene sequences and molecular hybridization involving the 3' noncoding regions. Arch. Virol. 122: 249-261.  https://doi.org/10.1007/BF01317187
  27. Tu, J. C. 1988. Bean yellow mosaic: now the most severe virus disease of white beans in southwestern Ontario. Annu. Rep. Bean Improv. Coop. 31: 143. 
  28. Uyeda, I., Takahashi, T. and Shikata, E. 1991. Relatedness of the nucleotide sequence of the 3'-terminal region of clover yellow vein potyvirus RNA to bean yellow mosaic potyvirus RNA. Intervirology 32: 234-245.  https://doi.org/10.1159/000150205
  29. Wylie, S. J., Coutts, B. A., Jones, M. G. K. and Jones, R. A. C. 2008. Phylogenetic analysis of bean yellow mosaic virus isolates from four continents: relationship between the seven groups found and their hosts and origins. Plant Dis. 92: 1596-1603.  https://doi.org/10.1094/PDIS-92-12-1596
  30. Wylie, S. J., Kueh, J., Welsh, B., Smith, L. J., Jones, M. G. K. and Jones, R. A. C. 2002. A non-aphid-transmissible isolate of bean yellow mosaic potyvirus has an altered NAG motif in its coat protein. Arch. Virol. 147: 1813-1820.  https://doi.org/10.1007/s00705-002-0846-y
  31. Yoon, J. Y., Cho, I. S., Chung, B. N. and Choi, S. K. 2022. First report of clover yellow vein virus on orchid (Dendrobium sp.) in South Korea. Plant Dis. 106: 1076. 
  32. Yoon, J.-Y., Choi, S.-K., Palukaitis, P. and Gray, S. M. 2011. Agrobacterium-mediated infection of whole plants by yellow dwarf viruses. Virus Res. 160: 428-434.  https://doi.org/10.1016/j.virusres.2011.06.026