대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제50권2호
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  • Pages.179-192
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  • 2024
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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앵두 추출물의 세포 수준에서의 스트레스 호르몬 생성 억제를 통한 GABA 조절 및 Filaggrin 과 Claudin-1 의 활성 효과

Effect of GABA Regulation and Activities of Filaggrin and Claudin-1 through Inhibiting Stress Hormone Production by Prunus tomentosa Extract In Vitro

  • 최원영 ((주)코씨드바이오팜 피부사랑임상연구센터) ;
  • 박성민 ((주)코씨드바이오팜 피부사랑임상연구센터) ;
  • 김라혜 ((주)코씨드바이오팜 피부사랑임상연구센터) ;
  • 이형진 ((주)코씨드바이오팜 피부사랑임상연구센터) ;
  • 이정노 ((주)코씨드바이오팜 피부사랑임상연구센터) ;
  • 유화선 ((주)코씨드바이오팜 피부사랑임상연구센터)
  • Won Yeoung Choi (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation) ;
  • Sung Min Park (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation) ;
  • Ra Hye Kim (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation) ;
  • Hyoung Jin Lee (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation) ;
  • Jung No Lee (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation) ;
  • Hwa Sun Ryu (SkinSaRang Clinical R&D Center, CoSeedBioPharm Corporation)
  • 투고 : 2024.05.24
  • 심사 : 2024.06.27
  • 발행 : 2024.06.30
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초록

본 연구에서는 천연물 6 종 앵두, 으름, 살구, 야콘, 금귤 및 핑거라임을 활용하여 스트레스 완화에 의한 수면 개선 및 피부장벽 개선 효과를 검증하였다. 실험 결과, 6 종의 천연물 중 앵두, 핑거라임, 으름 및 금귤이 유의적으로 스트레스 호르몬인 cortisol의 생성을 억제하였다. 또한, 수면 조절에 관여하는 GABA 생성 효소인 GAD67의 발현은 앵두 정제수 추출물 및 핑거라임 50% 에탄올 추출물에서 유의한 증가 효과를 보였으며 앵두 용매별 추출물은 총 폴리페놀의 함량이 가장 높게 확인되었다. 스크리닝 결과 활성이 가장 높은 앵두 추출물을 활용하여 후속 실험을 진행하였다. 앵두 용매별 추출물 중, 30% 에탄올 추출물이 가장 높은 DPPH radical 소거 활성을 보였으며, 정제수 추출물은 GABA 생성 및 피부 장벽 인자인 filaggrin과 claudin-1의 발현을 가장 높게 증가시켰다. HPLC 분석을 통해 앵두 추출물의 주성분으로 quercitrin이 확인되었고 추출 용매별 quercitrin 함량은 30% 에탄올 > 정제수 > 70% 에탄올 > 50% 에탄올의 추출 용매 순으로 높은 함량을 보였다. Quercitrin은 cortisol의 생성을 농도의존적으로 억제하였으며, cortisol에 의해 감소되었던 GAD67 발현 및 GABA 생성을 유의적으로 증가시켰다. 연구를 통해 앵두 추출물이 스트레스를 완화시켜 수면 개선 및 피부 장벽 강화에 도움을 주는 화장품 소재로서 활용이 가능함을 입증하였다.

In this study, six types of natural products, Prunus tomentosa (P. tomentosa), Akebia quinata (A. quinata), Prunus armeniaca (P. armeniaca), Smallanthus sonchifolius (S. sonchifolius), Citrus japonica (C. japonica), and Citrus australasica (C. australasica), were used to verify the effect of improving sleep and skin barriers by stress relief. As a result of the experiment, the production of cortisol, a stress hormone, was significantly inhibited by the P. tomentosa, C. australasica, A. quinata, and C. japonica among the six natural products. In addition, the expression of GAD67, a GABA-producing enzyme involved in sleep regulation, showed a significant increase in P. tomentosa purified water extract and C. australasica 50% ethanol extract, and the extract by each P. tomentosa solvent was found to have the highest total polyphenol content. Based on the results, the P. tomentosa extract with the highest activity was finally selected, and subsequent experiments were conducted. Among each P. tomentosa solvent extract, the DPPH radical scavenging activity was the highest in the 30% ethanol extract, and purified water extract increased GABA production and skin barrier factors filaggrin and claudin-1 expression the highest. HPLC analysis confirmed quercitrin as the main component of P. tomentosa extract, and quercitrin content by extraction solvent was high in the order of 30% ethanol > purified water > 70% ethanol > 50% ethanol. Quercitrin inhibited the production of cortisol in a concentration-dependent manner, significantly increasing GAD67 expression and GABA production, which had been reduced by cortisol. From the results of this study, it has been demonstrated that P. tomentosa can be used as a cosmetic material to help improve sleep and strengthen skin barriers by relieving stress.

키워드

과제정보

본 연구는 충북경제자유구역청 및 충북테크노파크의 충북형 산업혁신 뉴딜사업(충북혁신뉴딜을 위한 향기원료 개발 및 제품 사업화)의 연구비 지원을 받아 수행됨

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