Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Gut microbiota derived from fecal microbiota transplantation enhances body weight of Mimas squabs

  • Jing Ren (College of Animal Science, Jilin University) ;
  • Yumei Li (College of Animal Science, Jilin University) ;
  • Hongyu Ni (College of Animal Science, Jilin University) ;
  • Yan Zhang (College of Animal Science and Technology, Jilin Agricultural Science and Technology University) ;
  • Puze Zhao (College of Animal Science, Jilin University) ;
  • Qingxing Xiao (College of Animal Science, Jilin University) ;
  • Xiaoqing Hong (College of Animal Science, Jilin University) ;
  • Ziyi Zhang (College of Animal Science, Jilin University) ;
  • Yijing Yin (College of Animal Science, Jilin University) ;
  • Xiaohui Li (Center of Animal Experiment, College of Basic Medical Sciences, Jilin University) ;
  • Yonghong Zhang (College of Animal Science, Jilin University) ;
  • Yuwei Yang (College of Animal Science, Jilin University)
  • Received : 2023.11.09
  • Accepted : 2024.02.08
  • Published : 2024.08.01
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Abstract

Objective: Compared to Mimas pigeons, Shiqi pigeons exhibit greater tolerance to coarse feeding because of their abundant gut microbiota. Here, to investigate the potential of utilizing intestinal flora derived from Shiqi pigeons, the intestinal flora and body indices of Mimas squabs were evaluated after fecal microbiota transplantation (FMT) from donors. Methods: A total of 90 one-day-old squabs were randomly divided into the control group (CON), the low-concentration group (LC) and the high-concentration group (HC): gavaged with 200 μL of bacterial solution at concentrations of 0, 0.1, and 0.2 g/15 mL, respectively. Results: The results suggested that FMT improved the body weight of Mimas squabs in the HC and LC groups (p<0.01), and 0.1 g/15 mL was the optimal dose during FMT. After 16S rRNA sequencing was performed, compared to those in the CON group, the abundance levels of microflora, especially Lactobacillus, Muribaculaceae, and Megasphaera (p<0.05), in the FMT-treated groups were markedly greater. Random forest analysis indicated that the main functions of key microbes involve pathways associated with metabolism, further illustrating their important role in the host body. Conclusion: FMT has been determined to be a viable method for augmenting the weight and intestinal microbiota of squabs, representing a unique avenue for enhancing the economic feasibility of squab breeding.

Keywords

Acknowledgement

The authors thank Yumei Li, Yonghong Zhang, and Yuwei Yang for critical reading of the manuscript.

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