Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Pregnancy influences expression of interferon-stimulated genes, progesterone receptor and progesterone-induced blocking factor in ovine thyroid

  • Jianhua Cao (School of Life Sciences and Food Engineering, Hebei University of Engineering) ;
  • Shuxin Zhao (School of Life Sciences and Food Engineering, Hebei University of Engineering) ;
  • Yaqi Zhang (School of Life Sciences and Food Engineering, Hebei University of Engineering) ;
  • Jiabao Cai (School of Life Sciences and Food Engineering, Hebei University of Engineering) ;
  • Leying Zhang (School of Life Sciences and Food Engineering, Hebei University of Engineering) ;
  • Ling Yang (School of Life Sciences and Food Engineering, Hebei University of Engineering)
  • Received : 2023.12.01
  • Accepted : 2024.02.18
  • Published : 2024.08.01
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Abstract

Objective: Embryonic interferon-tau (IFNT) and progesterone affect expression of interferon-stimulated genes (ISGs), progesterone receptor (PGR) and progesterone-induced blocking factor (PIBF) in the ovine thyroid. Methods: Thyroids of ewes were sampled at day 16 of nonpregnancy, days 13, 16, and 25 of pregnancy, and real-time quantitative polymerase chain reaction assay, western blot and immunohistochemistry were used to detect expression of ISGs, PGR, and PIBF. Results: Free ISG15 protein was undetected, but ISG15 conjugated proteins upregulated at day 16 of pregnancy, and expression levels of ISG15 conjugated proteins, PGR isoform (70 kDa), PIBF, interferon-gamma-inducible protein 10 and myxovirusresistance protein 1 peaked, but expression level of signal transducer and activator of transcription 1 was the lowest at day 16 of pregnancy. In addition, the expression levels of PGR isoform (70 kDa) and signal transducer and activator of transcription 1 (STAT1) decreased, but levels of PGR isoform (43 kDa), 2',5'-oligoadenylate synthetase, IP-10 and MX1 increased at day 25 of pregnancy comparing with day 16 of the estrous cycle. Conclusion: Early pregnancy affects expression of ISGs, PGR, and PIBF in maternal thyroid through IFNT and progesterone, which may regulate thyroid autoimmunity and thyroid hormone secretion in ewes.

Keywords

Acknowledgement

This work was supported by the grants from Natural Science Foundation of Hebei Province, China (C2021402019), and Hebei Science and Technology Agency, China (22326602D).

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